We narrowed to 7,953 results for: mme
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pcDNA3.1 HisC EXO1b
Plasmid#68268PurposeMammalian expression of human EXO1DepositorInsertEXO1 (EXO1 Human)
TagsHisExpressionMammalianMutationb form, codons 1-846. Please see depositor commen…PromoterCMVAvailable SinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCI H2B-GFP
Plasmid#92399PurposeUbiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-EGFP reporter.DepositorInsertIRES H2B EGFP
ExpressionMammalianPromoterCAGAvailable SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSR348
Plasmid#124713PurposeExpression of narX under PtacDepositorUseSynthetic BiologyExpressionBacterialMutationS193* - LacI is truncated and likely deactivated.…Available SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-dDIO-vCre
Plasmid#118029PurposeAAV vector for sparse and bright labeling in a cell-type specific mannerDepositorInsertTRE-dDIO-vCre
UseAAVMutationSee depositor comments belowPromoterTREAvailable SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-Kdm6b(1025-End)_H1390A-pA
Plasmid#100279PurposeIn vitro transcription template (T7 promoter) for Kdm6b catalytic domain mutantDepositorInsertKdm6b (Kdm6b Mouse)
TagsFlag epitope tagExpressionMammalianMutationMutated catalytic domain (1025aa-End) (please see…Available SinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EGFP-P2A-FusionRed
Plasmid#191108PurposeMammalian cell line expression plasmids for production of EGFP and FusionRed proteinsDepositorInsertpAAV-CAG-EGFP-P2A-FusionRed
UseAAVTagsFusionRedExpressionMammalianMutationSee depositor comments belowPromoterchicken β-actin promoterAvailable SinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET29a-hNABP2
Plasmid#128307PurposeExpress hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tagDepositorInsertHuman Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)
TagsHisExpressionBacterialMutationY85C- please see depositor comments belowPromoterT7Available SinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFUW-tetO-NR4A3
Plasmid#139818Purposedoxycycline-inducible expression of Human NR4A3 in mammalian cellsDepositorInsertNR4A3 (NR4A3 Human)
UseLentiviralExpressionMammalianMutationS240G- please see depositor comment belowPromoterTRE-mCMVAvailable SinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEMI91
Plasmid#74888PurposeShuffled 9I27 polyprotein with shuffled codons to allow facile sequencing and unique RE for the 9 cassettes for versatile cloning. See Depositor Comments for more informationDepositorInsertTitin I27 x 9 (TTN Human)
TagsHis-tag and Strep-tag IIExpressionBacterialPromoterT7 promoterAvailable SinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBTK569
Plasmid#110614PurposeBTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by CP25 promoterDepositorInsertE2-Crimson
ExpressionBacterialMutationV32M (see comments below)Available SinceOct. 2, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAG-memRFP-3xControlgRNA
Plasmid#224567PurposeUbiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.DepositorInsertmRFP1
UseCRISPRTagsMembrane localization signalMutationCodon optimizedAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrc99S-ssDsbA-MBP-4xDQNAT
Plasmid#128398PurposePeriplasmic expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites in E. coliDepositorInsertMaltose binding protein
Tags4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…ExpressionBacterialMutationY342H- please see depositor commentsPromotertrcAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVenus-N-SLC35B2
Plasmid#155038PurposeTransient expression of SLC35B2-VenusDepositorInsertSLC35B2 (SLC35B2 Human)
TagsVenusExpressionMammalianMutationSee depositor comments belowPromoterCMVAvailable SinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
VVT1
Plasmid#65779PurposeThe Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNADepositorInsertSaCas9 gRNA backbone, without spacer sequence
UseCRISPRExpressionMammalianPromoterU6Available SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRB117.2
Plasmid#181949PurposeaTc-inducible expression of NarL with N-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuSDepositorInsertTagsmNeonGreenExpressionBacterialMutationSee Depositor Comments BelowPromotermNG-NarL-PLtetO-1; mCherry-PdcuS; tetR-J23106Available SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBMN mCherry-TAX1BP1(V142S/ΔUBD)
Plasmid#119690PurposeFor mammalian expression of LIR mutant TAX1BP1 without its UBDDepositorInsertTAX1BP1 (TAX1BP1 Human)
UseRetroviralTagsmCherryExpressionMammalianMutationV142S & UBD deletion (1-755), See depositor c…Available SinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF6 VPS51-V5-6His
Plasmid#175502PurposeV5 tagged VPS51 is expressed in mammalian cellsDepositorInsertVPS51 (VPS51 Human)
TagsV5ExpressionMammalianMutationA661V- please see depositor commentsAvailable SinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNXF-1-5xmut_O
Plasmid#147116PurposeMammalian Expression of HsNxf-1-5xmutDepositorAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only
