59,428 results

1. Emerin pEGFP-C1 (637)

   Plasmid

   #61993

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   Emerin (EMD Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

   Plasmid

   #182653

   Purpose

   Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Inserts

   codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
   PylT
   mutant eukaryotic release factor 1
   

   Tags

   HA tag and nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   E55D and Y306A/Y384F (AF) double mutant of Methan…

   Promoter

   CMV and U6

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL4 [luc2P/GAS-RE/Hygro] Vector

   Plasmid

   #236813

   Purpose

   Express GAS response element in luciferase reporter assay

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   GAS (GAST Human)
   

   Use

   Luciferase

   Mutation

   None

   Promoter

   minP

   Available Since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. Flag-HRD1

   Plasmid

   #231688

   Purpose

   Expresses human HRD1 protein

   Depositor

   Chunhong Ma

   Article

   Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

   Insert

   HRD1 (SYVN1 Human)
   

   Tags

   3×Flag Tag

   Expression

   Mammalian

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3-Sox2

   Plasmid

   #101761

   Purpose

   Sox2 promoter reporter

   Depositor

   Yuh-Shan Jou

   Article

   Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

   Insert

   SRY-box 2 promoter (SOX2 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1_puro_shNRF2-1

   Plasmid

   #230086

   Purpose

   shRNA silencing human NRF2 gene

   Depositor

   Xingguo Zhu

   Article

   Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

   Insert

   Nrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Jan. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. p16sheLL

   Plasmid

   #37320

   Depositor

   John Schiller

   Article

   Buck et al PLoS Pathog. 2006 Jul;2(7):e69.

   Insert

   HPV16L1+L2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV_3xFLAG-ATP6V0A3-mScarlet

   Plasmid

   #228867

   Purpose

   Stable expression of V-ATPase subunit ATP6V0A3 with N-terminal 3xFLAG and C-terminal mScarlet

   Depositor

   Wilhelm Palm

   Article

   Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

   Insert

   ATP6V0A3 (TCIRG1 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG and mScarlet

   Available Since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3- SREBP-1

   Plasmid

   #204192

   Purpose

   Mammalian expression of SREBP-1 (isoform 1)

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SREBP-1 (SREBF1 Human)
   

   Tags

   E-tag and T7x2

   Expression

   Mammalian

   Mutation

   multiple silent mutations

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-DEST47 PINK1 C-GFP

    Plasmid

    #13316

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 (PINK1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT7-7 asyn A53T

    Plasmid

    #105727

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Article

    Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

    Insert

    alpha-synuclein A53T (SNCA Human)
    

    Expression

    Bacterial

    Mutation

    A53T

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMVTNT-T7-KPNA7

    Plasmid

    #26683

    Depositor

    Bryce Paschal

    Article

    Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

    Insert

    Karyopherin Alpha 7 (KPNA7 Human)
    

    Tags

    T7

    Expression

    Mammalian

    Available Since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pINDUCER dCas9-TET1CD

    Plasmid

    #101921

    Purpose

    The dCas9-TET1CD fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948).

    Depositor

    Danwei Huangfu

    Article

    Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

    Insert

    dCas9-TET1CD
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-C1-AR

    Plasmid

    #28235

    Purpose

    Mammalian expression of Androgen receptor fused to EGFP

    Depositor

    Michael Mancini

    Article

    Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

    Insert

    Androgen receptor (AR Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. EF1a_FOXA2_P2A_Hygro_Barcode

    Plasmid

    #120439

    Purpose

    Barcoded lentiviral vector to express FOXA2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    FOXA2 (FOXA2 Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV_3XHA-ATP6V1B2

    Plasmid

    #228864

    Purpose

    Stable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA

    Depositor

    Wilhelm Palm

    Article

    Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

    Insert

    ATP6V1B2 (ATP6V1B2 Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pINDUCER dCas9-TET1CDMut

    Plasmid

    #101920

    Purpose

    The dCas9-TET1CDMut fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948). The TET1 catalytic domain is mutated to abolish catalytic function.

    Depositor

    Danwei Huangfu

    Article

    Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

    Insert

    dCas9-TET1CD Mut
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Mutated TET1 catalytic domain

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. dTAG-IRF8

    Plasmid

    #190620

    Purpose

    Lentiviral expression plasmid of human IRF8 fused with FKBP12G36V for dTAG-mediated degradation

    Depositor

    Junwei Shi

    Article

    Cao et al Mol Cell. 2021 Sep 2;81(17):3604-3622.e1

    Insert

    IRF8 (IRF8 Human)
    

    Use

    Lentiviral

    Tags

    FKBP12_G36V

    Expression

    Mammalian

    Mutation

    synonymous mutation for sgRNA resistance for sgIR…

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDisplay-GRAB_ATP1.0-IRES-mCherry-CAAX

    Plasmid

    #167582

    Purpose

    Express the ATP sensor GRAB_ATP1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

    Insert

    ATP sensor GRAB_ATP1.0 (P2RY1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV_BE4max

    Plasmid

    #112093

    Purpose

    C:G-to-T:A base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    BE4max
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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