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pLVX-M-Flag-BAP1

Plasmid

#125840

Purpose

express wild type BAP1

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

BAP1 (BRCA1 associated protein 1) (BAP1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mutation

Promoter

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-AlkB

Plasmid

#228218

Purpose

Express wild type AlkB protein in E. coli BL21(DE3)

Depositor

Junchao Shi

Article

Shi et al Nat Cell Biol. 2021 Apr;23(4):424-436. d

Insert

Alpha-ketoglutarate-dependent Dioxygenase (alkB Synthetic)

Use

Tags

His tag

Expression

Bacterial

Mutation

codon optimized

Promoter

T7

Available since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pF1-Hs Cdk1/cyclin B1

Plasmid

#177330

Purpose

Co-expression of the human active Cdk1/cyclin B1 kinase complex

Depositor

Andreas Boland

Article

Yu et al Nature. 2021 Aug;596(7870):138-142. doi:

Inserts

Cyclin-dependent kinase 1 (CDK1 Human)
G2/mitotic-specific cyclin-B1 (CCNB1 Human)

Use

Tags

2 x Strep-tag and 8 x His

Expression

Insect

Mutation

Promoter

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pSH624-ssr

Plasmid

#199241

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

single-stranded DNA recombineering

Use

Tags

Expression

Bacterial

Mutation

Promoter

lacIq-Ptrc

Available since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pC13N-iCAG.copGFP

Plasmid

#66578

Purpose

CLYBL safe harbor gene targeting donor expressing CAG-driven copGFP, compatible with pZT-C13 TALENs

Depositor

Jizhong Zou

Article

Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

Insert

copGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET21d-myc-BirA

Plasmid

#109424

Purpose

Bacterial expression of biotin ligase (BirA) with an N-terminal myc epitope

Depositor

Martin Spiess

Article

Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

BirA (birA )

Use

Tags

myc

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-SV40NLSf-mScarletP2A-hLuc

Plasmid

#218793

Purpose

AAV reporter genome plasmid

Depositor

Ben Deverman

Article

Huang et al Science. 2024 Jun 14;384(6701):1220-1227

Insert

NLSf-mScarletP2A-hLuc

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF2316

Plasmid

#142031

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ETV4 (ETV4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDisplay-NE2h-IRES-mCherry-CAAX

Plasmid

#208691

Purpose

Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h and a membrane-localized mcherry in mammalian cells

Depositor

Yulong Li

Insert

GPCR activation based norepinephrine (NE) sensor GRAB_NE2h

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Tet2

Plasmid

#60939

Purpose

Expresses Tet2 in mammalian cells

Depositor

Yi Zhang

Article

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Ten-Eleven Translocation 2 (Tet2 Mouse)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-ABE7.10-P2A-GFP

Plasmid

#162509

Purpose

Express ABE7.10-P2A-GFP by CMV promoter in mammalian cells

Depositor

Yongsub Kim

Article

Shin et al Nucleic Acids Res. 2021 Feb 26;49(4):239

Insert

ABE7.10-P2A-GFP

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pTUB1:OsTIR1-3FLAG, SAG1:CAT

Plasmid

#87258

Purpose

TIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.

Depositor

David Sibley

Article

Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d

Inserts

TIR1
CAT

Use

Toxoplasma expression

Tags

3FLAG

Expression

Mutation

Codon optimized for Toxoplasma gondii expression

Promoter

TgSAG1 361 bp and TgTUB1 2717 bp

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_Pembrolizumab_heavychain_T2A_ Pembrolizumab_lightchain_PGK_mCherry

Plasmid

#85434

Purpose

Lentiviral vector for Gal4 inducible leucinezipper Pembrolizumab and constitutive mCherry expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

Pembrolizumab

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pKM3

Plasmid

#154168

Purpose

Empty vector with FLAG tag expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

Depositor

Kevin Maringer

Article

Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Kir2.1WT-2A-mScarlet-KASH

Plasmid

#203840

Purpose

Kir2.1 expression for silencing neurons and mScarlet-KASH expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

Kir2.1 (Kcnj2 Mouse)

Use

AAV

Tags

2A-mScarlet-KASH

Expression

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pT3TS-spRYCas9-ABE-UMax

Plasmid

#222139

Purpose

Near PAMless Adenine base editor with modified TadA variant for A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

TadA-nSpRYCas9

Use

CRISPR; Zebrafish expression

Tags

bipartite NLS

Expression

Mutation

Promoter

T3

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pCI-neo:Beta2meGFP

Plasmid

#45096

Depositor

Henry Lester

Article

Srinivasan et al J Gen Physiol. 2011 Jan;137(1):59-79.

Insert

nAChR beta2 (Chrnb2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

There are strain differences between C57BJ/6 and …

Promoter

CMV

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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TetOn-REEP5-mCherry-eGFP

Plasmid

#139870

Purpose

Dox-inducible lentiviral expression of human REEP5

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

REEP5 (REEP5 Human)

Use

Lentiviral

Tags

mCherry-eGFP

Expression

Mutation

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCMU-PERr

Plasmid

#61202

Purpose

Fluorescent reporter for peroxisomes mCherry-SKL expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

mCherry-SKL

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

AtUBQ10

Available since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti6.2-3xFLAG-V5-ccdB

Plasmid

#87072

Purpose

Lentiviral Gateway-compatible expression vector backbone with a N-terminal 3xFLAG-V5 tag

Depositor

Mikko Taipale, Susan Lindquist

Article

Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

3xFLAG-V5

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-Tubulin-C-18

Plasmid

#55148

Purpose

Localization: Microtubules, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Fiolka et al Proc Natl Acad Sci U S A. 2012 Apr 3;109

Insert

Tubulin (TUBA1B Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
pCK015

Plasmid

#105347

Purpose

Bacterial translocation fo proteins into plant cells

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

secretion signal, ccdB cassette

Use

Bacterial secretion

Tags

Expression

Mutation

BsaI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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p350_LTJ_180bpCD90.1Template

Plasmid

#104988

Purpose

180bp HDR template for murine CD90.1

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

180bp genomic DNA sequence from murine CD90.1

Use

Hdr template for gene editing

Tags

Expression

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-PEmax-P2A-Puro

Plasmid

#228890

Purpose

Lentiviral delivery and expression of PEmax protein with puromycin selection cassette.

Depositor

Francisco J Sánchez-Rivera

Article

Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

Inserts

PEmax
Puromycin resistance

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAL750

Plasmid

#200988

Purpose

Inducible promoter Pxyl for Haloferax volcanii

Depositor

Alexandre Bisson

Article

Rados et al Front Microbiol. 2023 Aug 10;14:1204876.

Type

Empty backbone

Use

Expression in haloarchaea

Tags

Expression

Mutation

Promoter

Pfdx, Pxyl

Available since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-EZH2

Plasmid

#28060

Depositor

Mien-Chie Hung

Article

Wei et al Nat Cell Biol. 2011 Jan . 13(1):87-94.

Insert

EZH2 (EZH2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti-DHB-mVenus-p2a-mCherry-CDK4KTR

Plasmid

#126679

Purpose

Fluorescent reporter for cyclin E/A-CDK and CDK4/6 activities

Depositor

Hee Won Yang

Article

Yang et al Elife. 2020 Apr 7;9. pii: 44571. doi: 10

Insert

Rb (a.a 886-928)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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UbcH5B

Plasmid

#60443

Purpose

Express E2 UbcH5B in E. Coli

Depositor

Cynthia Wolberger

Article

Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

Insert

UbcH5B (UBE2D2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pCAIP-hXRCC1wt-eGFP

Plasmid

#206032

Purpose

Mammalian expression of hXRCC1wt coupled to eGFP under the control of a CAG promoter

Depositor

Primo Schaer

Article

Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

Insert

hXRCC1

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-myrAkt-P2A-BCL2

Plasmid

#233182

Purpose

Retroviral expression of myristoylated Akt1 (myr-Akt) and Bcl2

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

myr-Akt (Akt1, BCL2 Mouse, Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

myr

Promoter

Available since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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