We narrowed to 22,696 results for: Sis;

1. pCFJ150-Cas9(dpiRNA)

   Plasmid

   #107940

   Purpose

   eft-3p::Cas9(dpiRNA)::tbb-2 3'UTR construct used for MosSCI in nematode. Cas9 is optimized by removing all piRNA targeting sites to allow germline expression.

   Depositor

   Heng-Chi Lee

   Article

   Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

   Insert

   Cas9(dpiRNA)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   eft-3

   Available Since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMJA089

   Plasmid

   #128518

   Purpose

   Expresses human Lysozyme in Pichia pastoris

   Depositor

   Marcos Alcocer

   Article

   Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

   Insert

   Artificial gene for human lysozyme (LYZ Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGWB440

   Plasmid

   #74826

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with eYFP (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSCV eIF4E W73A IRES GFP

   Plasmid

   #18762

   Depositor

   Scott Lowe

   Article

   Wendel et al Genes Dev. 2007 Dec 15. 21(24):3232-7.

   Insert

   eIF4E W73A (Eif4e Mouse)
   

   Use

   Retroviral

   Tags

   IRES GFP

   Expression

   Mammalian

   Mutation

   W73A

   Promoter

   Available Since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pGEX-4T1-Cdc42 G15A

   Plasmid

   #69356

   Purpose

   expression of nucleotide free Cdc42 in bacterial cells

   Depositor

   Rafael Garcia-Mata

   Article

   Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

   Insert

   Cdc42 (CDC42 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   G15A

   Promoter

   tac

   Available Since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pWL89A mmPing20

   Plasmid

   #145788

   Purpose

   mPing Yeast Transposition Assay

   Depositor

   Charles Nathan Hancock

   Article

   Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

   Insert

   mmPing20
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   T162C, T258A, T287C, T300A, T304A, T310A, G372A

   Promoter

   Available Since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLIK 3xFLAG-TWIST2 neo

   Plasmid

   #197936

   Purpose

   Lentiviral expression vector for an inducible 3xFLAG-tagged TWIST2

   Depositor

   Kevin Janes

   Article

   Dutta et al Genome Res. 2023 Feb 21;33(3):314-31. do

   Insert

   TWIST2 (TWIST2 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDGE667

   Plasmid

   #153232

   Purpose

   Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

   Depositor

   Sylvestre Marillonnet

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   GFP

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGWB523

   Plasmid

   #74865

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with GST (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. FRB_nSbMVp

    Plasmid

    #118969

    Purpose

    Expresses N-terminus of soybean mosaic virus protease (SbMVp) fused to FKBP-rapamycin binding (FRB) domain

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split nSbMVp in fusion with FRB
    

    Use

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGWB507

    Plasmid

    #74849

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEPQD2KN0908

    Plasmid

    #177088

    Purpose

    MoClo Level 2 plasmid containing multiple transcriptional units for transient expression of P19 (cytosol, driven by nosP), along with CrDXS2, PaGPPS1, and CrGES (plastid, driven by 4x opaatB)

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    P19, CrDXS2, PaGPPS1, and CrGES
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. YFP-Rac1(L61)

    Plasmid

    #11401

    Depositor

    Joel Swanson

    Article

    Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

    Insert

    ras-related C3 botulinum toxin substrate 1 (RAC1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Obtained with Glutamine 61 mutated to Leucine.

    Promoter

    Available Since

    Feb. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

14. FUW-M2-PAK2 wt

    Plasmid

    #31680

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    p21-activated protein kinase 2 (PAK2 Human)
    

    Use

    Lentiviral

    Tags

    M2

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. optoPKAW196R/Y204A

    Plasmid

    #104546

    Purpose

    Expresses Cry2-mCherry-PKA W196R/Y204A in mammalian cells.

    Depositor

    David Lawrence

    Article

    O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

    Insert

    Cry2-mCherry-PKA W196R/Y204A
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    PKA W196R and Y204A

    Promoter

    CMV

    Available Since

    March 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGWB423

    Plasmid

    #74817

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with GST (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available Since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-CibN-dCas9

    Plasmid

    #60551

    Purpose

    Expresses CibN-dCas9 in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    CibN-dCas9
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    inactivating Cas9 mutations D10A and H840A

    Promoter

    CMV

    Available Since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHH0103_SNX18-1/1

    Plasmid

    #91369

    Purpose

    Protein expression and purification of human SH3 domain construct SNX18-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SNX18-1/1 (SNX18 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-4T1-Rac1 G15A

    Plasmid

    #69355

    Purpose

    expression of nucleotide free Rac1 in bacterial cells

    Depositor

    Rafael Garcia-Mata

    Article

    Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    G15A

    Promoter

    tac

    Available Since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pCS2+ macroH2A1.2-GFP-HA

    Plasmid

    #30515

    Depositor

    John Gurdon

    Article

    Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

    Insert

    macroH2A1.2-C (Macroh2a1 Mouse)
    

    Use

    Xenopus/zebrafish expression.

    Tags

    eGFP and triple HA

    Expression

    Mammalian

    Mutation

    NO STOP

    Promoter

    Available Since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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21. pJMP1223

    Plasmid

    #119261

    Purpose

    rfp "test" strain

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available Since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. pJMP1221

    Plasmid

    #119260

    Purpose

    rfp "test" strain

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available Since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLVX puro TRIM21 C54Y - GFP

    Plasmid

    #116942

    Purpose

    Expression of TRIM21 C54Y -GFP fusion

    Depositor

    Gaudenz Danuser

    Article

    Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

    Insert

    TRIM21 C54Y-GFP (TRIM21 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C54Y

    Promoter

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA5/FRT/TO DNAJB4

    Plasmid

    #19472

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB4 (DNAJB4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLVXPuro-epsilon-COP-GFP

    Plasmid

    #66604

    Purpose

    Expresses epsilon COP as a GFP fusion

    Depositor

    David Stephens

    Article

    Morohashi et al Biochem J. 2010 Apr 14;427(3):401-12. do

    Insert

    epsilon COP (COPE Bovine)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

26. YFP-PCAF-C2

    Plasmid

    #134572

    Purpose

    Expresses YFP-PCAF fusion protein

    Depositor

    Susan Janicki

    Article

    Rafalska-Metcalf et al PLoS One. 2010 Apr 21;5(4):e10272. doi:

    Insert

    PCAF (Kat2b Mouse)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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27. pET23a-HsUBB

    Plasmid

    #69562

    Purpose

    Bacterial expression; in vitro transcription/translation (T7)

    Depositor

    Jorge Eduardo Azevedo

    Article

    Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

    Insert

    UBB (UBB Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pCK004 ubi:mCerulean_SV40 (#302)

    Plasmid

    #195978

    Purpose

    Expression construct

    Depositor

    Christian Mosimann, Alexa Burger

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    ubi:mCerulean
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Lenti_CRISPRi_sgNeg1

    Plasmid

    #166998

    Purpose

    Lentiviral expression of negative control sgRNA for CRISPRi knockdown

    Depositor

    Gian-Paolo Dotto

    Article

    Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

    Insert

    Negative control sgRNA 1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDest Lenti CA-GFP

    Plasmid

    #197459

    Purpose

    Bicistronic expression vector expressing CA-GFP under the CMV promotor separated by an internal ribosome entry site (IRES) mediated expression of dTomato reporter protein.

    Depositor

    Omar Khan

    Article

    Keyan et al Cancers (Basel). 2023 Jan 13;15(2):505.

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    S65T

    Promoter

    CMV, IRES

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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