We narrowed to 51,167 results for: ANG

1. PTPN13_PDZ_4

   Plasmid

   #103922

   Purpose

   Protein expression and purification of PTN13 PDZ4 domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   PTPN13 (PTPN13 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGC(2.1)-U6.3

   Plasmid

   #170516

   Purpose

   PCR template vector for amplifying gRNAcore(2.1)-pU6.3 promoter fragment; used together with pAC-CR7T-gRNA2.1-nlsBFP

   Depositor

   Chun Han

   Article

   Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

   Insert

   gRNAcore(2.1)-pU6.3
   

   Use

   Pcr template vector for amplifying grnacore(2.1)-…

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CaMKII-ASAP2s

   Plasmid

   #101275

   Purpose

   Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

   Depositor

   Francois St-Pierre

   Article

   Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

   Insert

   ASAP2s
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAMKIIa

   Available Since

   Nov. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+) M-Ras(WT)

   Plasmid

   #224287

   Purpose

   Cell transfection and expression of M-Ras

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   M-Ras (MRAS Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR-D-TOPO-kcnn3

   Plasmid

   #164959

   Purpose

   Zebrafish kcnn3 gene CDS

   Depositor

   GuangJun Zhang

   Article

   Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

   Insert

   kcnn3 (kcnn3 Zebrafish)
   

   Use

   Pcr cloning vector

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3 Flag FKHR H215R AAA mutant

   Plasmid

   #13510

   Depositor

   Kunliang Guan

   Article

   Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

   Insert

   FKHR H215R AAA (FOXO1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   H215R T24A S256A S319A

   Available Since

   Dec. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

7. OA-1067A1

   Plasmid

   #164847

   Purpose

   Expresses four gRNA targeting BTub gene with 3xp3-eGFP marker

   Depositor

   Omar Akbari

   Article

   Li et al Nat Commun. 2021 Sep 10;12(1):5374. doi:

   Insert

   4 gRNAs and 3xP3-eGFP
   

   Expression

   Insect

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET15b-eGO-Matryoshka-LE-LP

   Plasmid

   #100017

   Purpose

   Bacterial expression of fluorescent protein casette - cpEGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   cpEGFP-LSSmOrange
   

   Tags

   6x HIS tag

   Expression

   Bacterial

   Mutation

   Leu and Glu N-term linker and Leu and Pro C-term …

   Promoter

   T7

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Venus-7aa-ActA-pEGFP-C1

   Plasmid

   #177405

   Purpose

   To express Venus fused to mitochondria outer membrane targeting sequence "ActA". Venus faces the cytosol.

   Depositor

   David Andrews

   Article

   Osterlund et al Protocol Exchange (2022)

   Insert

   ActA tail anchor (actA Listeria monocytogenes)
   

   Tags

   Venus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-P1-LoxP-Neo-N-miRFP670-C

    Plasmid

    #159434

    Purpose

    CRISPR knock-in donor construct with fluorescent reporter and removable selectable marker

    Depositor

    Jianhua Xing

    Article

    Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

    Inserts

    Neomycin
    miRFP670
    

    Use

    CRISPR

    Tags

    3' linker pair 1, 5' linker pair 1, C t…

    Promoter

    SV40

    Available Since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Direct-seq_lentiGuide-Puro-Loop2-8A8G

    Plasmid

    #157983

    Purpose

    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

    Depositor

    Lijia Ma

    Article

    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

    Insert

    S. pyogenes sgRNA cassette (Loop2-8A8G)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. p15a_Kan_MLS_Rosa26_Short_CRISPRDel_FNeoStop_PStop_WPRE_bgh_pA

    Plasmid

    #97012

    Purpose

    Rosa26 targeting vector for intersectional Cre and FLP responsive cassette

    Depositor

    Russell Ray

    Article

    Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Cre/Lox

    Expression

    Mammalian

    Available Since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-dLight1.3a

    Plasmid

    #111055

    Purpose

    Expresses dLight1.3a in mammalian cells

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Insert

    dLight1.3a
    

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pR008_APAD

    Plasmid

    #204818

    Purpose

    Expression Apobec1-ADAR2dd fusion protein as PIE-RBP control

    Depositor

    Xiaochang Zhang

    Article

    Ruan et al Nat Commun. 2023 Jun 6;14(1):3275. doi:

    Insert

    rat Apobec1 and human ADAR2 deaminase domain with engineered sites (Apobec1 Rat)
    

    Tags

    HA and Flag and huADAR2dd

    Expression

    Mammalian

    Promoter

    chicken β-actin promoter

    Available Since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV CB6 FFLuc-miR122

    Plasmid

    #35656

    Depositor

    Phillip Zamore

    Article

    Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

    Insert

    pri-miR-122 (Mir122 )
    

    Use

    AAV

    Promoter

    CB

    Available Since

    April 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS18

    Plasmid

    #158787

    Purpose

    Ribosome-tagged GCaMP (soma localized) expression pan-neuronally C. elegans

    Depositor

    Jennifer Garrison, Zachary Knight

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    Ptag-168::GCaMP6m::rpl-1a
    

    Expression

    Worm

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-TDAG51

    Plasmid

    #32699

    Depositor

    Richard C. Austin

    Article

    Hossain et al J Biol Chem. 2003 Aug 8;278(32):30317-27

    Insert

    human tdag51 (PHLDA1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

18. OA-1067K

    Plasmid

    #164848

    Purpose

    Expresses four gRNA targeting Mhc gene with 3xp3-tdTomato marker

    Depositor

    Omar Akbari

    Article

    Li et al Nat Commun. 2021 Sep 10;12(1):5374. doi:

    Insert

    4 gRNAs and 3xP3-tdTomato
    

    Expression

    Insect

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. OA-874PA

    Plasmid

    #164846

    Purpose

    Expresses AAEL005635-Cas9 with OpIE2-CFP marker

    Depositor

    Omar Akbari

    Article

    Li et al Nat Commun. 2021 Sep 10;12(1):5374. doi:

    Insert

    P10-3'UTR OpIE2-CFP
    

    Expression

    Insect

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. LITE1.0_pAAV_hSyn_CRY2PHR-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

    Plasmid

    #47454

    Purpose

    epiLITE / LITE1.0 CRY2PHR fused to SID4X transcriptional repressor effector domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

    Insert

    CRY2PHR-NLS-SID4X
    

    Use

    AAV

    Tags

    2A_phiLOV2.1

    Expression

    Mammalian

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available Since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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21. pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40

    Plasmid

    #133560

    Purpose

    Backbone vector for CRISPR-HDR insertion of SPARC-Variant constructs (or any module) near the attP40 locus

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. (282) pcDNA3.1-HA-nGFP-(EAAAK)4-OGT(4,K852A)

    Plasmid

    #160741

    Purpose

    Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to a GFP nanobody

    Depositor

    Christina Woo

    Article

    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

    Insert

    HA-nGFP-(EAAAK)4-OGT(4,K852A)
    

    Tags

    HA-Tag

    Expression

    Mammalian

    Promoter

    T7/CMV

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. tdp43-EGFP construct11

    Plasmid

    #28204

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa193-274 only

    Available Since

    Sept. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

24. pGIPz-PD-L1 3SA

    Plasmid

    #121490

    Purpose

    Expression of human PD-L1 3SA with PD-L1 shRNA

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Inserts

    PD-L1 3SA
    humun PD-L1 shRNA
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S176A, T180A, S184A

    Promoter

    CMV

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pFastBacDual with LFn + FlaAAA

    Plasmid

    #84867

    Purpose

    Baculovirus Expression of LFn-FlaAAA fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.

    Depositor

    Russell Vance

    Article

    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

    Inserts

    LFn
    FlaAAA
    

    Use

    Baculovirus

    Tags

    6xHis

    Expression

    Insect

    Mutation

    3 C-terminal leucines have been replaced by alani…

    Promoter

    Polyhedrin

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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26. p164-pAAV-mDlx-FLEX-mScarlet

    Plasmid

    #204689

    Purpose

    Fluorescent reporter under the control of the Dlx promotor

    Depositor

    Lynette Lim

    Article

    Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

    Insert

    mScarlet
    

    Use

    AAV and Cre/Lox

    Tags

    mScarlet palmitoylation

    Expression

    Mammalian

    Promoter

    mDlx

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. PL752-CLSTN2-LA-p2a-tdTomato-hGHpA-PGK-puroR-CLSTN2-RA

    Plasmid

    #191685

    Purpose

    For knocking p2a-tdTomato into CLSTN2 locus

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    CLSTN2 homology arms and p2a-tdTomato
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. pBAD-R-iLACCO1.1

    Plasmid

    #208020

    Purpose

    Biosensor for intracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    R-iLACCO1.1
    

    Expression

    Bacterial

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. pSLQ10714

    Plasmid

    #183959

    Purpose

    U6-crKlf4-CAG-hyperdCas12a-miniVPR

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Inserts

    HyperdCas12a
    crRNA targeting Klf4
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D832A, D156R, E292R, D235R, D350R

    Promoter

    CAG and U6

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. pUAST-SIK3 T196E

    Plasmid

    #52975

    Purpose

    insect expression of SIK3 T196E constitutively active mutant

    Depositor

    Marc Montminy, Biao Wang

    Article

    Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

    Insert

    SIK3 (Sik3 Fly)
    

    Tags

    HA

    Expression

    Insect

    Mutation

    T196E constitutively active mutant

    Promoter

    Hsp70

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only