173,383 results
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Plasmid#213296PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only
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pJO629
Plasmid#104342PurposeExpress yeast Yap5-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.DepositorInsertpTDH3tc3-Yap5-3xFLAG-tCyc1-pTDHtc3-flagDHFRha-tCyc1 (YAP5 Budding Yeast)
Tags3xFLAGExpressionYeastAvailable SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EFS-BirAopt-GSQ-RNF4ca-P2A-BLAST
Plasmid#208047PurposeEnables constitutive expression of N-terminal BirAopt-fused RNF4ca (catalytically inactive RING mutant); to perform bioE3; selection with blasticidinDepositorInsertRNF4 (RNF4 Human)
UseLentiviralTagsBirAoptMutationCys>Ala mutations in conserved RING domainPromoterEFSAvailable SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
JDW 742 (pDONR221-EGFP-KRAS4B-G12V)
Plasmid#156403PurposeGateway middle entry clone encoding EGFP-fused to human KRAS4B with G12V mutation, includes a stop codonDepositorInserthuman mutant KRAS4B-G12V (KRAS Human)
UseGateway CloningTagsEGFPMutationmutant KRAS4B-G12VAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
Anti-GABA(A)R, Alpha2 [N399/19R]
Plasmid#177544PurposeMammalian Expression Plasmid of anti-GABA(A)R, Alpha2 (Rat). Derived from hybridoma N399/19.DepositorInsertanti-GABA(A)R, Alpha2 (Rattus norvegicus) recombinant mouse monoclonal antibody (Gabra2 Mouse)
ExpressionMammalianPromoterDual CMVAvailable SinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
AEB-No-aptamer-controlmRFP1
Plasmid#63848PurposeThis pFTV1 vector contains a no-aptamer control mRNA encoding mRFP1 gene. It should be used when testing AEB-F12 and AEB-Theo45 riboswitches to measure their non-specific ligand effect on mRFP1 expression.DepositorInsertNo-aptamer control mRNA
UseSynthetic BiologyPromoterJ23100Available SinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAP27
Plasmid#194706PurposeLinker::3xFlag::mNeonGreen::HAtag::LinkerDepositorInsertLinker::3xFlag::mNeonGreen::HAtag::Linker
UseSynthetic BiologyAvailable SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2778
Plasmid#144157PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Geph CR shRNA
Plasmid#121068PurposeshRNA targeting the coding region of the gephyrin mRNADepositorInsertGPHN shRNA (Gphn Rat)
UseRNAiAvailable SinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
Mitofusin-1 scFv [N111/24]
Plasmid#211603PurposeMammalian Expression Plasmid of Mitofusin-1 scFv. Derived from hybridoma N111/24.DepositorInsertMitofusin-1 (Rattus norvegicus) recombinant mouse scFv. (Mfn1 Mouse)
ExpressionMammalianPromoterCMVAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only