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We narrowed to 5,994 results for: crispr cas9 expression plasmids

Showing: 1401 - 1440 of 5994 results

  1. BPK764

    Plasmid
    #65767
    Purpose
    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65767-plasmid-map-sequence-id-471631

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  3. BPK2101

    Plasmid
    #65770
    Purpose
    Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized SaCas9, and SaCas9 gRNA
    Use
    CRISPR
    Tags
    NLS-3xFlag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65770-plasmid-map-sequence-id-112650

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  4. pKLV2-U6(gDmap1 v1)-PGK-Puro-BFP

    Plasmid
    #117140
    Purpose
    Express gRNA vs Dmap1
    Depositor
    Kosuke Yusa
    Insert
    gDmap1 v1 (Dmap1 Synthetic)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Deleted BbsI site within WPRE, modified BFP
    Promoter
    human U6 promoter, mouse PGK promoter
    Available since
    April 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    117140-plasmid-map-sequence-id-233768

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  7. VP12

    Plasmid
    #72247
    Purpose
    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A and Q926A
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72247-plasmid-map-sequence-id-131866

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  8. BPK2797

    Plasmid
    #72251
    Purpose
    Human expression plasmid for SpCas9-VRQR variant: CMV-T7-humanSpCas9-VRQR(D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR(D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335Q, and T1337R
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72251-plasmid-map-sequence-id-131872

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  9. MSP2372

    Plasmid
    #73196
    Purpose
    Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A, Q695A, and Q926A in SpCas9
    Promoter
    CMV
    Available since
    Feb. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73196-plasmid-map-sequence-id-134659

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  10. pCB-CBEv2

    Plasmid
    #221139
    Purpose
    Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 2 without sgRNA construct. Expresses 3xF-BE4-PpAPOBEC1(H122A).
    Depositor
    Craig Roy
    Article
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    3xF-PpAPOBEC1(H122A)-Cas9n-UGI-UGI (BE4-PpAPOBEC1(H122A))
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Bacterial
    Mutation
    Promoter
    lacIq-Ptac
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221139-plasmid-map-sequence-id-437031

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  11. pCB-CBEv1

    Plasmid
    #221138
    Purpose
    Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 1 without sgRNA construct. Expresses 3xF-HF-BE3.
    Depositor
    Craig Roy
    Article
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    3xF-rAPOBEC1-Cas9n(HF)-UGI (HF-BE3)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Bacterial
    Mutation
    Promoter
    lacIq-Ptac
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221138-plasmid-map-sequence-id-445118

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  14. hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid

    Plasmid
    #154429
    Purpose
    hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid
    Depositor
    David Liu
    Article
    Koblan et al Nature. 2021 Jan;589(7843):608-614. doi:
    Insert
    hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    VRQR point mutations in SpCas9
    Promoter
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    154429-plasmid-map-sequence-id-304585

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  15. pJAT11

    Plasmid
    #204290
    Purpose
    For inserting a phiC31 attP site. HDR integrant is marked with removable ie1-DsRed cassette, which is expressed in abdomen and mouthparts.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-DsRed
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ie1
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204290-plasmid-map-sequence-id-405507

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  16. pJAT75

    Plasmid
    #204306
    Purpose
    Flippase under heat shock control. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-EGFP
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ie1
    Available since
    Aug. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    204306-plasmid-map-sequence-id-406175

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  17. pJAT62

    Plasmid
    #204301
    Purpose
    Express phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-EGFP
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ie1
    Available since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204301-plasmid-map-sequence-id-405845

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  18. pJAT43

    Plasmid
    #204299
    Purpose
    For introducing heat shock inducble B3 recombinase. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-EGFP
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ie1
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204299-plasmid-map-sequence-id-406188

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  19. pJAT56

    Plasmid
    #204300
    Purpose
    Express phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    3XP3-EGFP
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    3XP3
    Available since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204300-plasmid-map-sequence-id-405836

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  21. pML104-KanMx4

    Plasmid
    #83476
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.
    Depositor
    Patricia Wittkopp
    Insert
    KanMX4
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    83476-plasmid-map-sequence-id-189195

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  22. pML104-HygMx4

    Plasmid
    #83475
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.
    Depositor
    Patricia Wittkopp
    Insert
    HphMX4
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    83475-plasmid-map-sequence-id-358352

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  23. pML104-NatMx3

    Plasmid
    #83477
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains NatMX3 marker for yeast transformation.
    Depositor
    Patricia Wittkopp
    Insert
    NatMx3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only

  24. pAAV-SCP1-dSa-VPR mini.

    Plasmid
    #99685
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Use
    AAV
    Tags
    VPR mini
    Expression
    Mutation
    dead Cas9
    Promoter
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    99685-plasmid-map-sequence-id-194598

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  25. pAAV-EFS-dSa-VPR mini.

    Plasmid
    #99686
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from EFS promoter
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Use
    AAV
    Tags
    VPR mini
    Expression
    Mutation
    dead Cas9
    Promoter
    Available since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    99686-plasmid-map-sequence-id-194608

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  26. pUDP082

    Plasmid
    #103875
    Purpose
    Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces marxianus
    Depositor
    Jean-Marc Daran
    Article
    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4
    Insert
    HH-gRNA-HDV targetting ADE2 in Kluyveromyces marxianus
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    103875-plasmid-map-sequence-id-199262

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  27. pUDP025

    Plasmid
    #103874
    Purpose
    Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces lactis
    Depositor
    Jean-Marc Daran
    Article
    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4
    Insert
    HH-gRNA-HDV targetting ADE2 in Kluyveromyces lactis
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    103874-plasmid-map-sequence-id-199263

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  28. pUDP013

    Plasmid
    #103873
    Purpose
    Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Ogataea (para)polymorpha
    Depositor
    Jean-Marc Daran
    Article
    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4
    Insert
    HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    103873-plasmid-map-sequence-id-199239

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  29. pSB1C3-FeGFP

    Plasmid
    #138261
    Purpose
    Donor-eGFP vector to be used in plasmid to plasmid integration reporter assay. eGFP is conditionally expressed when integrated at iCas9-sites on acceptor plasmid
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10
    Insert
    eGFP
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    None
    Available since
    March 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    138261-plasmid-map-sequence-id-273630

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  30. pFC334

    Plasmid
    #87846
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymes
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    argB
    yA specific sgRNA
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    A. nidulans gpdA promoter with Hammerhead ribozym…
    Available since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    87846-plasmid-map-sequence-id-174613

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  31. pDIV489

    Plasmid
    #177697
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.
    Depositor
    Uffe Mortensen
    Article
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    RNR2p (Debaryomyces hansenii ) and TDH3p (Candida…
    Available since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    177697-plasmid-map-sequence-id-352587

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  32. pDIV490

    Plasmid
    #177698
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.
    Depositor
    Uffe Mortensen
    Article
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    RHR2p (Debaryomyces hansenii ) and TDH3p (Candida…
    Available since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    177698-plasmid-map-sequence-id-352588

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  33. pDIV492

    Plasmid
    #177700
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.
    Depositor
    Uffe Mortensen
    Article
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    RHR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    177700-plasmid-map-sequence-id-366535

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  34. pDIV491

    Plasmid
    #177699
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.
    Depositor
    Uffe Mortensen
    Article
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    RNR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …
    Available since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    177699-plasmid-map-sequence-id-353309

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  35. pDIV488

    Plasmid
    #177696
    Purpose
    Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species
    Depositor
    Uffe Mortensen
    Article
    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0
    Inserts
    Cas9
    Nat
    Use
    CRISPR
    Tags
    SV40
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    TDH3p (Candida lusitaniae) and TEF1p (Ashbya goss…
    Available since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    177696-plasmid-map-sequence-id-352586

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  37. pORFE0001

    Plasmid
    #112070
    Purpose
    AtCas9 module CDS1
    Depositor
    Christian Chevalier
    Insert
    AtCAS9
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized Cas9 S. pyogenes
    Promoter
    Available since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112070-plasmid-map-sequence-id-217624

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  38. pML104

    Plasmid
    #67638
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation
    Depositor
    John Wyrick
    Article
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pSNR52 and pTDH3
    Available since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67638-plasmid-map-sequence-id-322882

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  39. pML107

    Plasmid
    #67639
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation
    Depositor
    John Wyrick
    Article
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pSNR52 and pTDH3 (aka GAP promoter)
    Available since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67639-plasmid-map-sequence-id-220324

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  40. 3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2 probasin_mTQ2_FlpO

    Plasmid
    #68405
    Purpose
    The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex8 and SMAD4 ex2. are expressed by the U6 promoter
    Depositor
    Jannik Elverløv-Jakobsen
    Inserts
    3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2
    FlpO
    Use
    CRISPR
    Tags
    mTurquoise2 (BFP)
    Expression
    Mammalian
    Mutation
    Promoter
    Synthetic Probasin ARRx2 and U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68405-plasmid-map-sequence-id-119955

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Showing: 1401 - 1440 of 5994 results