We narrowed to 12,086 results for: HAL;

1. CoV2-N-R203K

   Plasmid

   #177951

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203K). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (R203K) (N )
   

   Expression

   Mammalian

   Mutation

   R203K

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCCM

   Plasmid

   #162708

   Purpose

   Second CCM operon cloned from H. neapolitanus; pFA expressing the remaining 11 genes in the H. neapolitanus CCM cluster

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   Second CCM operon cloned from H. neapolitanus
   

   Expression

   Bacterial

   Promoter

   PLtet0-1 promoter

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-flag Mst2 (KR)

   Plasmid

   #27372

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

   Insert

   Mst2 (STK3 Human)
   

   Tags

   flag

   Expression

   Mammalian

   Mutation

   K56R (catalytically inactive)

   Available Since

   Feb. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

4. SynNotch TMD/Gal4-VP64 ICD (MTK 3b) (pAN1031)

   Plasmid

   #194222

   Purpose

   Mammalian Toolkit part 3b encoding SynNotch and GAL4-VP64 to be used in designing modular receptors

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   SynNotch-GAL4 DBD-VP64 AD
   

   Use

   Synthetic Biology

   Promoter

   None

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSL0812 (pCRISPR(BbsI)_Vch)

   Plasmid

   #200898

   Purpose

   Expresses V. cholerae CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   VchCRISPR (entry)
   

   Expression

   Mammalian

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEY26

   Plasmid

   #191031

   Purpose

   cho-1(3.3-2.6k) fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   cho-1(3.3-2.6k) (cho-1 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEY39

   Plasmid

   #191043

   Purpose

   acr-2(2.1k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   acr-2(2.1k) (acr-2 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCV-FKBP-RFP(pLxIS-PLPLR)

   Plasmid

   #221284

   Purpose

   Expression of RFP (pLxIS-PLPLR) in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   RFP (pLxIS-PLPLR) (Mkrn1 Synthetic)
   

   Use

   Retroviral

   Tags

   3xFLAG-4xFKBP

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCV-FKBP-TRIF(Nterm)

   Plasmid

   #221244

   Purpose

   Expression of TRIF N-terminus (1-222) in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   TRIF N-terminus (1-222) (Ticam1 Mouse)
   

   Use

   Retroviral

   Tags

   3xFLAG-4xFKBP

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJY-RpABE-PDS3_IMS

    Plasmid

    #112875

    Purpose

    binary vector for IMS (Induced Mis-Splicing) of PDS3 in Arabidopsis

    Depositor

    Jin-Soo Kim, Jae-Young Yun

    Article

    Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

    Inserts

    RPS5A promoter
    ABE7.10
    U6-sgRNA targeting PDS3
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRW004-SM-destination-proUBQ10-Cas9

    Plasmid

    #104438

    Purpose

    Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;

    Depositor

    Detlef Weigel

    Article

    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial and Plant

    Promoter

    UBQ10

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBR322-MCK1.35lux

    Plasmid

    #53368

    Purpose

    expresses luciferase under 1.35 kb muscle creatine kinase promoter/enhancer

    Depositor

    Josephine Nalbantoglu

    Article

    Larochelle et al Gene Ther. 1997 May;4(5):465-72.

    Insert

    muscle creatine kinase promoter/enhancer 1.35kb
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pAAV-flex-GFP-shRNA-scramble

    Plasmid

    #182503

    Purpose

    Expresses scramble shRNA for control expts. Flexed cassette driven by pan neuronal gene promoter

    Depositor

    William Wisden

    Article

    Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

    Insert

    pan neuronal promoter driving flexed GFP-shRNA scramble from mir 30 cassette
    

    Use

    AAV, Cre/Lox, and RNAi

    Expression

    Mammalian

    Promoter

    hSyn promoter

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Bact2-APEX2-GBP in pDEST-Tol2-pA2-acrys-cherry

    Plasmid

    #67668

    Purpose

    Zebrafish expression vector for ubiquitous expression of APEX2-GBP

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    APEX2-GBP
    

    Use

    Zebrafish expression with tol2

    Available Since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2-mRuby-Rab8a

    Plasmid

    #194337

    Purpose

    Expresses mRuby Rab8a in mammalian cells and zebrafish embryos

    Depositor

    Heidi Hehnly

    Article

    Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

    Insert

    Rab8 (RAB8A Canis lupus)
    

    Tags

    mRuby

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOPINK-OTUD3 (OTU+UBA, aa 52-275)

    Plasmid

    #61411

    Purpose

    Expresses human OTUD3 (OTU+UBA domains) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD3 (OTUD3 Human)
    

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-51 and aa 276-398.

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. CoV2-N-G204R

    Plasmid

    #177953

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (G204R). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (G204R) (N )
    

    Expression

    Mammalian

    Mutation

    G204R

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOPINK-OTUD5 (OTU, aa 171-358)

    Plasmid

    #61415

    Purpose

    Expresses human OTUD5 (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD5 (OTUD5 Human)
    

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-170 and aa 359-571.

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-Flag-YAP-S109A

    Plasmid

    #33088

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    YAP (YAP1 )
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S109A

    Promoter

    CMV

    Available Since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pEBG-PELP1

    Plasmid

    #65209

    Purpose

    Full length mammalian expression vector for hPELP1

    Depositor

    Ratna Vadlamudi

    Article

    Nair et al Cancer Res. 2010 Sep 15;70(18):7166-75.

    Insert

    PELP1 (PELP1 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only