We narrowed to 11,713 results for: NSI

1. pPBpuro-miRFP703-eDHFR(69K6)-cRaf

   Plasmid

   #184717

   Purpose

   iRaf

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

   Insert

   miRFP703-eDHFR(69K6)-cRaf (RAF1 Synthetic, Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBoBi-hALDOA K230A

   Plasmid

   #210808

   Purpose

   To block the formation of the initial Schiff base with FBP

   Depositor

   Sheng-cai Lin

   Article

   Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

   Insert

   ALDOA (ALDOA Human)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   residues 230 of ALDOA mutated to alanine

   Promoter

   CMV

   Available Since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETDUET-GST AO7RE ∆238-245

   Plasmid

   #139318

   Purpose

   GST tagged AO7 encoding aa 126-258 with an internal deletion that results in loss of E2 binding for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 aa 126-258 (RNF25 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   AO7 cloned into the first multiple cloning site o…

   Promoter

   T7 promoter-1

   Available Since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. p202-Syt1-C2A-I248V

   Plasmid

   #201554

   Purpose

   Expresses Octopus synaptotagmin-1 C2A domain with I248V mutation in E. coli

   Depositor

   Joshua Rosenthal

   Article

   Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

   Insert

   Synaptotagmin-1 C2A domain (partial)
   

   Expression

   Bacterial

   Mutation

   changed Isoleucine 248 to Valine (numbering based…

   Promoter

   RBS

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc5.1-V5-HisB-ObKHC-K282R-HaloTag

   Plasmid

   #201552

   Purpose

   Expresses Octopus kinesin-1 motor with K282R mutation with HaloTag in Drosophila S2 cells

   Depositor

   Joshua Rosenthal

   Article

   Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

   Insert

   Kinesin-1
   

   Tags

   HaloTag

   Expression

   Insect

   Mutation

   changed lysine 282 to arginine (numbering based o…

   Promoter

   Ac5

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. hARL15_32-197

   Plasmid

   #209799

   Purpose

   Bacterial expression of human ARL15 GTPase domain

   Depositor

   Kalle Gehring

   Article

   Mahbub et al Elife. 2023 Jul 14;12:e86129. doi: 10.75

   Insert

   ARL15 (ARL15 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   aa 32-197 only

   Promoter

   T7

   Available Since

   Nov. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti7-3_EF1ap-mRuby2_hU6p_sgSTK4i_#1 (BlasR)

   Plasmid

   #209776

   Purpose

   CRISPRi for STK4

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   Nluc, sgSTK4i, mRuby2 (STK4 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV_dCas9-KRAB_sgSTK4i_#1 (PuroR)

   Plasmid

   #209773

   Purpose

   CRISPRi for STK4

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   dCas9-KRAB, sgSTK4i (STK4 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV_dCas9-KRAB_sgSTK4i_#2 (PuroR)

   Plasmid

   #209774

   Purpose

   CRISPRi for STK4

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   dCas9-KRAB, sgSTK4i (STK4 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAS_4xKBstTyrT(CUA)_EF1_sfGFP 102TAG 150TAA

    Plasmid

    #174895

    Purpose

    amber and ochre dual suppression reporter sfGFP 102TAG 150 TAA expression, with BstTyr(CUA) amber suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sfGFP
    

    Expression

    Mammalian

    Mutation

    102TAG 150TAA in sfGFP

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pINDUCER21-puro_TAOK2-D151A

    Plasmid

    #172980

    Purpose

    doxycycline-inducible expression of kinase-dead TAOK2

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    kinase-dead TAOK2 (TAOK2-D151A) (TAOK2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D151A

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#1

    Plasmid

    #172983

    Purpose

    CRISPRi for TAOK2

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    sgTAOK2i (TAOK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKG-GW2-NgBR

    Plasmid

    #203165

    Purpose

    Yeast expression vector for hNUS1

    Depositor

    William Sessa

    Article

    Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

    Insert

    NUS1 (NUS1 Human)
    

    Expression

    Yeast

    Promoter

    TDH3

    Available Since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. mEos3.2trLAT-P

    Plasmid

    #203747

    Purpose

    Encodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. The palmitoylation site at C27 is mutated to A. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    trLAToC27A (Lat Mouse)
    

    Tags

    ER translocation signal - mEos3.2

    Expression

    Mammalian

    Mutation

    C27A

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. trLATm1PtoLmEos3.2

    Plasmid

    #203749

    Purpose

    Encodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Prolines P8 and P17 in the LAT transmembrane domain mutated to L. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    trLATm1 (PtoL) (LAT Human)
    

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    P8L, P17L

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV_hSyn1_AdoLightG

    Plasmid

    #187465

    Purpose

    Expresses green adenosine indicator AdoLightG in neuronal cells

    Depositor

    Tommaso Patriarchi

    Article

    Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

    Insert

    AdoLightG
    

    Use

    AAV

    Tags

    Flag tag

    Promoter

    human Synapsin-1

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-mCherry-SPASTIN, L177D

    Plasmid

    #180649

    Purpose

    Lentiviral expression vector for SPASTIN. Has L177D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-45.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPASTIN
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Starts at M87, L177D mutation, has siRNA resistan…

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-THTAP-VPS9D1, residues 1-97

    Plasmid

    #180640

    Purpose

    Bacterial expression for VPS9D1 MIT domain, residues 1-97. Has N-terminal HIS-GST tag. Internal ID: WISP20-89.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    VPS9D1 (C16orf7 Human)
    

    Tags

    HIS-GST

    Expression

    Bacterial

    Mutation

    Residues 1-97

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-Myc-KATNB1

    Plasmid

    #160054

    Purpose

    Mammalian expression vector for KATNB1, (Katanin p80) with N-terminal Myc tag. Internal ID: WISP20-16.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    KATNB1 (KATNB1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCA528 KATNA1 MIT residues 1-79

    Plasmid

    #180614

    Purpose

    Bacterial expression for KATNA1 MIT domains, residues 1-79. Has N-terminal HIS-SUMO tag. Internal ID: WISP19-20.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    KATNA1 (KATNA1 Human)
    

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 1-79

    Promoter

    T7

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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