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Showing: 7221 - 7240 of 157662 results

  1. pMH-SFB

    Plasmid
    #99391
    Purpose
    Gateway-compatible mammalian expression destination vector for creating SFB N-terminal fusions (S protein tag, Flag tag, Streptavidin binding peptide)
    Depositor
    Michael Huen
    Article
    An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114
    Type
    Empty backbone
    Use
    Tags
    SFB (S protein-FLAG-Streptavidin binding peptide)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    99391-plasmid-map-sequence-id-194202

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  3. pDHt/sk-CE

    Plasmid
    #92128
    Purpose
    Expression of codon-optimized Cas9-eGFP fusion protein with Pcbh1 promoter in Trichoderma reesei
    Depositor
    Zhihua Zhou
    Article
    Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1
    Insert
    Cas9-eGFP
    Use
    CRISPR; Trichoderma reesei expression
    Tags
    EGFP
    Expression
    Mutation
    Promoter
    Pcbh2
    Available since
    Nov. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    92128-plasmid-map-sequence-id-195044

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  12. BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9)

    Plasmid
    #101178
    Purpose
    Human expression plasmid for SpCas9 Cluster 1 variant (HypaCas9): CMV-T7-hSpCas9-Cluster1(N692A, M694A, Q695A, H698A)-NLS(SV40)-3xFLAG
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster1(N692A/M694A/Q695A/H698A)
    Use
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    N692A/M694A/Q695A/H698A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101178-plasmid-map-sequence-id-219907

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  13. pet30b-7M SrtA

    Plasmid
    #51141
    Purpose
    expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity
    Depositor
    Hidde Ploegh
    Insert
    S.aureus SrtA 7M
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    P94R, E105K, E108Q, D160N, D165A, K190E, K196T
    Promoter
    T7
    Available since
    Feb. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    51141-plasmid-map-sequence-id-211598

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  14. pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3

    Plasmid
    #171588
    Purpose
    Lentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoter
    Depositor
    Kenneth Matreyek
    Article
    Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.
    Inserts
    Bxb1 integrase
    iCasp9
    BFP
    rtTA3G
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171588-plasmid-map-sequence-id-335078

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  17. TFORF2660

    Plasmid
    #142350
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    STAT1 (STAT1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142350-plasmid-map-sequence-id-397203

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  19. pAAV-nEF-Con/Fon-ChRmine-oScarlet

    Plasmid
    #137159
    Purpose
    Intersectional viral expression of ChRmine-p2a-oScarlet in cells expressing both Cre AND Flp
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Has Service
    AAV8
    Insert
    Con/Fon-ChRmine-p2a-oScarlet
    Use
    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt
    Tags
    Expression
    Mutation
    oScarlet E95D
    Promoter
    nEF
    Available since
    June 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    137159-plasmid-map-sequence-id-272775

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Showing: 7221 - 7240 of 157662 results