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pLenti_PEmax-2A-MLH1dn-2A-BSD

Plasmid

#234072

Purpose

For lentiviral expression of PEmax with MLH1dn and blasticidin resistance

Depositor

Greg Findlay

Article

Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

Insert

PEmax-2A-MLH1dn

Use

CRISPR and Lentiviral

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Promoter

EF-1α core promoter

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pyEvolvR (enCas9-PolI5M)

Plasmid

#158073

Purpose

Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

John Dueber

Article

Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

Insert

enCas9-PolI5M

Expression

Bacterial and Yeast

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcdna3.1 hciap1

Plasmid

#8311

Depositor

Jon Ashwell

Article

Li et al Nature 2002 Mar 21;416(6878):345-7.

Insert

cIAP1 (BIRC2 Human)

Tags

myc

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pLSL.R.Ly

Plasmid

#227697

Purpose

Reengineered geminiviral replicon vector

Depositor

Jae-Yean Kim

Article

Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

Type

Empty backbone

Expression

Plant

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pET-Neq2X7

Plasmid

#182366

Purpose

Expresses Neq2X7 DNA polymerase

Depositor

Morten Norholm

Article

Hernandez-Rollan et al BMC Biotechnol. 2024 Apr 2;24(1):17. doi

Insert

Neq2X7 DNA polymerase

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pG106 - KA

Plasmid

#178041

Purpose

Bacteroides - Escherichia coli shuttle vector with ErmF and ErmAM, Kan and Amp selection markers

Depositor

Janina Lewis

Article

Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

Type

Empty backbone

Use

Shuttle vector

Expression

Bacterial

Available Since

Feb. 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223-PRKAB1

Plasmid

#23360

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKAB1 (PRKAB1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pLenti6.2_mCherry_ mito_roGFP2-Orp1

Plasmid

#155042

Purpose

Fluorescent redox reporter

Depositor

Vanessa LaPointe

Insert

Orp1 (HYR1 Budding Yeast)

Use

Lentiviral

Tags

mitochondrial targeting sequence and roGFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-Blasticidin

Plasmid

#75085

Purpose

retrovirus-mediated gene transfer into mammalian cells

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pGL4.23-GW

Plasmid

#60323

Purpose

This is a modified version of the pGL4.23 vector from Promega, containing a Gateway cassette upstream of the minimal promoter. This vector can be used for for Gateway cloning of candidate enhancer elements. As negative control, use pGL4.23 without the gateway cassette (available from Promega).

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Type

Empty backbone

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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lenti-NLS-mTagBFP2

Plasmid

#216128

Purpose

Lentiviral vector for expressing mTagBFP2 localizing to the nucleus

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Insert

EF1a-NuclearLocalizationSignal-mTagBFP2-WPRE

Use

Lentiviral

Expression

Mammalian

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pgRNA-CGG

Plasmid

#108248

Purpose

Lentiviral vector to express sgRNA targeting "GGCGGCGGCGGCGGCGGCGG" locus by SpCas9

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

Insert

sgRNA targeting GGCGGCGGCGGCGGCGGCGG

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin-Flpo

Plasmid

#60663

Purpose

Expresses pAAV-hSynapsin-Flpo

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

Flpo

Use

AAV

Promoter

hSynapsin1

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Floxed-STOP mCherry

Plasmid

#122963

Purpose

mCherry-based fluorescent reporter for Cre-loxP DNA recombination reaction

Depositor

Moritoshi Sato

Article

Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

Insert

pcDNA3.1_Floxed-STOP mCherry

Use

Cre/Lox

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-NF2-CFP

Plasmid

#235687

Purpose

Express CFP tagged NF2 gene in mammalian cells using the CMV promoter

Depositor

Duojia Pan

Article

Wang et al Cell. 2022 Nov 10;185(23):4376-4393.e18.

Insert

NF2 (CA12 Human)

Use

Lentiviral

Tags

CFP

Expression

Mammalian

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5 HIS-Ub-K48R

Plasmid

#221368

Purpose

Expresses 6xHIS-tagged Ubiquitin containing K48R mutation in mammalian cells

Depositor

Aga Gambus

Article

Jones et al iScience. 2024 Jun 12;27(7):110260. doi:

Insert

Ubiquitin

Tags

6xHIS

Expression

Mammalian

Mutation

Changed Lysine 48 to Arginine

Promoter

CMV

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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phND2-N106

Plasmid

#31780

Depositor

Jerry Crabtree

Article

Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

Insert

NeuroD2 (NEUROD2 Human)

Use

Lentiviral

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pLacO-ISce1

Plasmid

#58505

Purpose

provides 256 Lac operator repeats adjacent to the ISce1 restriction site

Depositor

Tom Misteli

Article

Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

Insert

Lac repeats adjacent to 6 copies of the ISce1 restriction site

Expression

Bacterial

Promoter

no promoter

Available Since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCas9-sgAAVS1-1

Plasmid

#129726

Purpose

Expressing SpCas9 and sgAAVS1-1

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

SpCas9 and sgAAVS1-1

Use

CRISPR

Tags

P2A-Puro

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIA II

Plasmid

#52093

Purpose

Retroviral expression of all four WT CD3 subunits, co-expressed with mAmetrine

Depositor

Dario Vignali

Article

Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:

Inserts

CD3 delta (Cd3d Mouse)
CDC gamma (Cd3g Mouse)
CD3 epsilon (Cd3e Mouse)
CD3 zeta (Cd247 Mouse)

Use

Retroviral

Tags

F2A, IRES-EGFP, P2A, and T2A

Expression

Mammalian

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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