We narrowed to 20,436 results for: ace
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Plasmid#227309PurposeDonor template for mStayGold insertion into the C-terminus of the COX8A locus. For mitochondria visualization. To be co-transfected with sgRNAplasmid px330-PITCh-COX8A (Addgene #227308)DepositorInsertCOX8A Homology Arms flanking a mStayGold Tag (COX8A Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-COX8A
Plasmid#227308PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of COX8A for knock-in.DepositorInsertsgRNA Targeting C-terminus of COX8A (COX8A Human)
UseCRISPRExpressionMammalianPromoterU67Available SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-A24
Plasmid#135507PurposeMammalian expression of myc-tagged HLA-A*024:02DepositorInsertHLA-A*24:02 (HLA-A Human)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianPromoterCMVAvailable SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO/GFP-TTP
Plasmid#141190PurposeExpresses GFP-TTP in mammalian cells, can be used to make inducible cell lineDepositorAvailable SinceJune 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-PLZF
Plasmid#61543PurposeExpresses PLZF in mammalian cellsDepositorAvailable SinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV-synaptophysin-Lime
Plasmid#201016PurposeExpresses Lime in synaptic vesicle lumen by fusion to synaptophysinDepositorAvailable SinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hMC4R
Plasmid#179315PurposeExpress human melanocortin 4 receptor (GFP tagged) in mammalian cellsDepositorAvailable SinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-Lhx2
Plasmid#61537PurposeExpresses Lhx2 in mammalian cellsDepositorAvailable SinceJan. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-3*Flag-APEX2 C-term 3NLS
Plasmid#187588PurposeExpress FLAG epitope and APEX2-tagged 3xNLS fusion protein in mammalian cellsDepositorInsertNLS
TagsAPEX2-FLAGExpressionMammalianPromoterCMVAvailable SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
BcLOV4 fusion tools screening plasmid #1
Plasmid#174511PurposeCloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP in a pcDNA3.1 backbone.DepositorInsert[BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP
TagsmCherryExpressionMammalianPromoterCMVAvailable SinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-cDNA5-FRT/TO-3*Flag-APEX2 N-term
Plasmid#182925PurposeExpress FLAG epitope and APEX2-tagged fusion protein in mammalian cells.DepositorTypeEmpty backboneTagsAPEX2 and FLAGExpressionMammalianPromoterCMVAvailable SinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
BcLOV4 fusion tools screening plasmid #2
Plasmid#174512PurposeCloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP in a pcDNA3.1 backbone.DepositorInsertplasmid B [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP
Tags3xFLAG and mCherryExpressionMammalianPromoterCMVAvailable SinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T7-6h-GST-α-Synuclein
Plasmid#225224PurposeAlpha synuclein gene fused with gst gene under t7 promoter for bacterial expression of alpha synuclein protein.DepositorInsertsGST
α-Synuclein
Tags6xHis Tag and TEV Cleavage SiteExpressionBacterialPromoterT7Available SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
(114) pcDNA3.1-GFP-Flag-JunB-EPEA
Plasmid#160743PurposeExpresses a GFP tagged JunB with Flag and EPEA tags.DepositorAvailable SinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-tetOn-IRES-G418 (EtO)
Plasmid#84776PurposeSecond generation lenti tetracycline operator (on)DepositorInsertTET OPERATOR
UseLentiviralPromoterpTIGHTAvailable SinceNov. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST-cDNA5-FRT/TO-3*Flag-APEX2 C-term
Plasmid#182926PurposeExpress FLAG epitope and APEX2-tagged fusion protein in mammalian cells.DepositorTypeEmpty backboneTagsAPEX2 and FLAGExpressionMammalianPromoterCMVAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-shRNA
Plasmid#82697PurposeFor targeting shRNA construct into human AAVS1 locus, using genome editing. Expresses shRNA of interest (cloning: EcoRI and AgeI) under U6 promoter, flanked by AAVS1 homology arms.DepositorTypeEmpty backboneUseRNAiPromoterhPGK, U6Available SinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
LAP2 Full I pAcGFP-N1 monomeric GFP (1317)
Plasmid#62044Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAnc80L65
Plasmid#68837PurposeAdeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct. Note that an improved version is available: Addgene plasmid# 92307. See comments for detailsDepositorInsertAncestral AAV Capsid Anc80L65
UseAAV and Synthetic BiologyExpressionMammalianPromoterRep2Available SinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHPHS232
Plasmid#228356PurposeLanding pad with Bxb1 attP_wildtype site, Dox-inducible BFP-iCasp9-Blasticidin, CMV-driven rtTA3, T2A-neomycin for HDR into AAVS1 locusDepositorInsertsmTagBFP2
Reverse tetracycline transactivator 3
neo
TagsBlasticidin S deaminase and iCasp9ExpressionMammalianPromoterTRE3GV and gene trapAvailable SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only