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177,651 results

Showing: 7281 - 7300 of 177651 results

  4. pPB-US-ECasE

    Plasmid
    #83961
    Purpose
    Piggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.
    Depositor
    Eleanor Chen
    Insert
    ERT2-spCas9-ERT2
    Expression
    Mammalian
    Mutation
    please see depositor comments below
    Promoter
    U6 (gRNA), EF1a (ERT2-Cas9-ERT2)
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    83961-plasmid-map-sequence-id-214594

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  5. pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)

    Plasmid
    #85008
    Purpose
    For Flpe/FRT-based Supernova-GFP, pK068 should be used with pK036. The GFP fragment of pK068 can be replaced with another gene by SalI and EcoRV.
    Depositor
    Takuji Iwasato
    Publication
    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP-ires-tTA
    Expression
    Mammalian
    Available since
    Nov. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    85008-plasmid-map-sequence-id-167314

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  6. pFUSEss-CHIg-mG1_M18

    Plasmid
    #82357
    Purpose
    Expression plasmid coding for heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18.
    Depositor
    Joanna Bereta
    Publication
    Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103
    Insert
    mouse immunoglobulin heavy chain IgG1 isotype (Ighg1 Mouse)
    Tags
    none
    Expression
    Mammalian
    Mutation
    none
    Promoter
    hEF1-HTLV prom
    Available since
    Sept. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    82357-plasmid-map-sequence-id-157227

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  10. pETM6-G6-vioABE-4A6-vioC-3A2-vioD

    Plasmid
    #73440
    Purpose
    Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.
    Depositor
    Mattheos Koffas
    Publication
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    PG6-vioABE-P4A6-vioC-P3A2-vioD
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    PG6, P4A6, and P3A2 (orthogonal T7-lac variants)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    73440-plasmid-map-sequence-id-135339

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  11. pHLmMBP-8

    Plasmid
    #72347
    Purpose
    Mammalian expression of cytoplasmic mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Publication
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Tags
    6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…
    Expression
    Mammalian
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Available since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    72347-plasmid-map-sequence-id-133755

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  12. pcDNA3.1+ eiF4A1 myc HIS

    Plasmid
    #71657
    Purpose
    mammalian expression of eiF4A1
    Depositor
    Gideon Dreyfuss
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eiF4A1 (EIF4A1 Human)
    Tags
    HIS and myc
    Expression
    Mammalian
    Mutation
    aa403 from Ala to Arg
    Promoter
    CMV
    Available since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    71657-plasmid-map-sequence-id-422687

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  16. CXCR4-Tango

    Plasmid
    #66262
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CXCR4 (CXCR4 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    9 Articles
    66262-plasmid-map-sequence-id-148151

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  18. BPK2101

    Plasmid
    #65770
    Purpose
    Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized SaCas9, and SaCas9 gRNA
    Use
    CRISPR
    Tags
    NLS-3xFlag
    Expression
    Bacterial
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65770-plasmid-map-sequence-id-112650

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  19. pcDNA3-PM-TORCAR

    Plasmid
    #64930
    Purpose
    Plasma membrane-targeted FRET biosensor for monitoring mTORC1 kinase activity.
    Depositor
    Jin Zhang
    Publication
    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyn-TORCAR (EIF4EBP1 Synthetic, Human)
    Tags
    Cerulean, N-terminal targeting sequence from Lyn …
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    64930-plasmid-map-sequence-id-109694

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  20. Clover-mRuby2-FRET-10

    Plasmid
    #58169
    Purpose
    Localization: Cytosolic FRET, Excitation: , Emission:
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    Clover and mRuby2
    Expression
    Mammalian
    Available since
    April 28, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    4 Articles
    58169-plasmid-map-sequence-id-200604

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Showing: 7281 - 7300 of 177651 results