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SRE reporter vector_559

Plasmid

#82686

Purpose

Cloning vector containing serum response element (SRE) followed by an AscI restriction site

Depositor

Harald Janovjak

Article

Reichhart et al Angew Chem Int Ed Engl. 2016 May 17;55(2

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pET16b-PCNA

Plasmid

#134898

Purpose

Bacterial expression and purification of human PCNA

Depositor

Andrew Deans

Article

Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

Insert

PCNA (PCNA Human)

Tags

untagged

Expression

Bacterial

Mutation

fully synthetic, codon optimized for bacterial ex…

Promoter

T7

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pBS mShh (CT#258)

Plasmid

#13999

Depositor

Cliff Tabin

Insert

Shh (Shh Mouse)

Expression

Bacterial

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pBBRBB-eGFP

Plasmid

#32549

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

His

Expression

Bacterial

Promoter

Consitutive lac

Available Since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pCAG-EGFP

Plasmid

#89684

Purpose

pCAG promoter driving EGFP

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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FRB-mCherry-FTH1

Plasmid

#100749

Purpose

Part of FerriTag expression system. Encodes human Ferritin with an FRB domain and mCherry tag

Depositor

Stephen Royle

Article

Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

Insert

ferritin heavy chain 1 (FTH1 Human)

Tags

FRB and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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CA-RIT-NFAT1 (IRES-GFP)

Plasmid

#85181

Purpose

Constitutively active NFAT1, mutated to interfere selectively with the NFAT:AP-1 interaction. Co-expresses EGFP for selection

Depositor

Anjana Rao

Article

Martinez et al Immunity. 2015 Feb 6. pii: S1074-7613(15

Insert

CA-RIT-NFAT1 (Nfatc2 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Amino acids 1-3 removed; CA: PASSGSSASF mutated t…

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only
LV-YFP

Plasmid

#26000

Depositor

Elaine Fuchs

Article

Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

Insert

H2B-YFP (H2BC21 A. victoria, Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 10, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA-Sumo1 WT

Plasmid

#48966

Purpose

Expresses HA-Sumo1 WT in mammalian cells

Depositor

Guy Salvesen

Article

Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

Insert

SUMO1 (SUMO1 Human)

Tags

HA

Expression

Mammalian

Promoter

T7

Available Since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pSL21-VEX

Plasmid

#158230

Purpose

Retroviral expression plasmid of sgRNA with violet-excited fluorescent protein (VEX)

Depositor

Junwei Shi

Article

Chen et al Cell. 2021 Mar 4;184(5):1262-1280.e22. d

Type

Empty backbone

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

U6 promoter for crRNA expression and EFS promoter…

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-Puro-2A

Plasmid

#102611

Purpose

Control lentivector expressing Puro resistance and gene of interest from CMV promoter

Depositor

Nicolas Manel

Article

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

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GAL4-DrBphP

Plasmid

#158986

Purpose

Expresses DrBphP fused with an N-terminal GAL4 DNA binding domain in mammalian cells

Depositor

Liangcai Gu

Article

Huang et al ACS Synth Biol. 2020 Nov 12. doi: 10.102

Insert

GAL4-DrBphP

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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LBR EGFP

Plasmid

#128150

Purpose

Expression of lamin B receptor

Depositor

Jennifer Lippincott-Schwartz

Article

Ellenberg et al J Cell Biol. 1997 Sep 22. 138(6):1193-20

Insert

lamin B receptor (LBR Human)

Expression

Mammalian

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2E3

Plasmid

#15789

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2E3 (UBE2E3 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pEG BacMam N term StrepII eGFP 3C

Plasmid

#160683

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Tags

StrepII eGFP 3C

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRS404-PTEF-Ago1

Plasmid

#22313

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Oct 23;326(5952):544-550.

Insert

AGO1

Use

Integrating plasmid

Expression

Yeast

Available Since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-SF2

Plasmid

#99021

Purpose

Mammalian expression of SF2

Depositor

Honglin Chen

Article

Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

Insert

SF2 (SRSF1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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Talin-TS

Plasmid

#83376

Purpose

FRET probe for measuring tension on Talin molecule

Depositor

Martin Schwartz

Article

Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d

Insert

Talin1 (Tln1 Mouse)

Use

Retroviral

Tags

EGFP-F40-tagRFP (tension sensor module)

Expression

Mammalian

Mutation

Tension sensor module is inserted between V447 an…

Promoter

CMV

Available Since

Oct. 26, 2016

Availability

Academic Institutions and Nonprofits only

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C1(1-29)-TurboID-V5_pLX304

Plasmid

#107175

Purpose

expresses V5-tagged TurboID on the mammalian ER membrane, lentiviral vector

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID (BirA mutant)

Use

Lentiviral

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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Plasmid L5 attB::Pleft* mScarlet

Plasmid

#169410

Purpose

Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mScarlet

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Inserts

pLeft
mScarlet proper

Use

Unspecified

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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