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pACEBac1-ZZ-TEV-MZT2-3C-mEGFP

Plasmid

#178074

Purpose

Insect cell expression of ZZ-TEV-MZT2-3C-mEGFP

Depositor

Tarun Kapoor

Article

Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

Insert

MZT2A (MZT2A Human)

Tags

PreScission-mEGFP and ZZ-TEV

Expression

Insect

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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P2RY8-Tango

Plasmid

#66477

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

P2RY8 (P2RY8 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pGEX6P1‐hsRNASEH2BCA

Plasmid

#108692

Purpose

For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzyme

Depositor

Andrew Jackson, Martin Reijns

Article

Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

Inserts

RNASEH2B (RNASEH2B Human)
RNASEH2C (RNASEH2C Human)
RNASEH2A (RNASEH2A Human)

Tags

GST

Expression

Bacterial

Mutation

Shine Dalgarno sequence upstream of start codon a…

Promoter

tac

Available Since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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MAC_C_PTH2

Plasmid

#111674

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

PTH2 (PTRH2 Human)

Expression

Mammalian

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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R989-X08-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C

Plasmid

#159540

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C (KRAS Human)

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

G12C

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-miR21

Plasmid

#21114

Depositor

Joshua Mendell

Article

Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

Insert

miR-21 (MIR21 Human)

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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FSW-ALDH2-T2A-EGFP

Plasmid

#197036

Purpose

Express ALDH2 from hSyn1 promoter, co-expressed with EGFP

Depositor

Ronald Hart

Article

Sun et al Aging Cell. 2023 Feb;22(2):e13772. doi:

Insert

ALDH2 (ALDH2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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Flag-HA-BAP1

Plasmid

#22539

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

BAP1 (BAP1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pCX-HO1-2A-EGFP

Plasmid

#74672

Purpose

Mammalian expression of HO1 and EGFP

Depositor

Roberto Giovannoni

Article

Cinti et al PLoS One. 2015 Oct 29;10(10):e0141933. d

Insert

HO1 (HMOX1 Human)

Tags

2A-EGFP

Expression

Mammalian

Promoter

pCAGGS

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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p53 (dominant negative R175H mutant)-pcw107-V5

Plasmid

#64638

Purpose

3rd generation lentiviral transfer plasmid. When used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

TP53 (transcript variant 3) (TP53 Human)

Use

Lentiviral

Tags

V5

Mutation

R175H

Promoter

PGK

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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V5-APEX2-SENP2

Plasmid

#129276

Purpose

Expression of APEX2 at nuclear pore

Depositor

Alice Ting

Article

Fazal et al Cell. 2019 Jun 14. pii: S0092-8674(19)30

Insert

V5-APEX2-SENP2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV promoter

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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EK0397 3FLAG-ADAR1-p150-HIS (pEAQ)

Plasmid

#191172

Purpose

Human ADAR1, p150 isoform in plant-expressable format.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

3FLAG-ADAR1-p150-HIS (plant) (ADAR Human)

Tags

3FLAG and HIS

Expression

Plant

Mutation

WT

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-Myr-Flag-AKT1

Plasmid

#15294

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

AKT1 (AKT1 Human)

Use

Retroviral

Tags

Myr-Flag

Expression

Mammalian

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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CCR4-Tango

Plasmid

#66241

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

CCR4 (CCR4 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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plk1 WT GFP-myc

Plasmid

#132963

Purpose

To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

Depositor

Michael Yaffe

Article

Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

Insert

polo-like kinase 1 (PLK1 Human)

Expression

Mammalian

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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GSN-bio-His

Plasmid

#52067

Purpose

Expresses full-length Gelsolin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

GSN (GSN Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pSCD-Casp3-L-tev-S

Plasmid

#226230

Purpose

Express TEVP-cleavable caspase-3

Depositor

Michael Elowitz

Article

Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

Insert

Caspase-3 (CASP3 Human)

Expression

Mammalian

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL Blast myc

Plasmid

#10674

Depositor

William Hahn

Article

Boehm et al Mol Cell Biol. 2005 Aug . 25(15):6464-74

Insert

c-myc (MYC Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pEF-hSEMA4C-Fc

Plasmid

#74449

Purpose

Expresses human Semaphorin-4C ectodomain fused to human Fc in mammalian cells

Depositor

Roland Friedel

Article

Le et al Oncotarget. 2015 Mar 30;6(9):7293-304.

Insert

human Semaphorin-4C ectodomain with human Fc tag (SEMA4C Human)

Tags

human Fc tag

Expression

Mammalian

Promoter

EF1-alpha

Available Since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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pGEx-4T-1-YTHDF1

Plasmid

#70087

Purpose

Encode YTHDF1 with N-terminal GST tag for E. coli expression

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTH N(6)-methyladenosine RNA binding protein 1 (YTHDF1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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