159,461 results

1. pCHW101020

   Plasmid

   #222454

   Purpose

   Level 1, Position 2 containing 35S::CUP2-p65::NOS.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   35S::CUP2-p65::NOS (CUP2 )
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   no

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Str-Ii_SBP-mCherry-Golgin84

   Plasmid

   #65304

   Purpose

   synchronize trafficking of Golgin-84 from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Golgin-84 (GOLGA5 Human)
   

   Use

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ER-mNeonGreen

   Plasmid

   #137804

   Purpose

   Visualization of the Endoplasmic Reticulum

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv 160374

   Insert

   KDEL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSLQ4419 pHR (hU6-BsmBI-BsmBI-EFS-PuroR-WPRE)

   Plasmid

   #214875

   Purpose

   Empty lentiviral backbone for guide array cloning

   Depositor

   Stanley Qi

   Article

   Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTP955

   Plasmid

   #104164

   Purpose

   Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (3x Cysteines)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   Anti-rabbit IgG Fc specific nanobody TP897 (3xCysteines)
   

   Use

   Tags

   14xHistidine tag and NEDD8 from Brachypodium dist…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AAV-Syn-DIO-TRPV1-P2A-mCherry

   Plasmid

   #200831

   Purpose

   In the presence of Cre, drives neuron-specific expression of TRPV1 with fluorescence reporter mCherry

   Depositor

   Hong Chen

   Article

   Yang et al Brain Stimul. 2021 Jul-Aug;14(4):790-800

   Insert

   TRPV1-P2A-mCherry (Trpv1 Rat)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. enOgeuIscB with ωRNA-v13

   Plasmid

   #220940

   Purpose

   CMV-driven expression of enOgeuIscB and human U6-driven expression of ωRNA-v13 scaffold with BsmBI for guide cloing

   Depositor

   Weixin Tang

   Article

   Yan et al Nat Chem Biol. 2024 Jul 8. doi: 10.1038/

   Inserts

   CMV-SV40 NLS-enOgeuIscB_nucleoplasmin NLS
   OgeuIscB-ωRNA-v13 scaffold with BsmBI for guide cloning
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAG-GFP-CD63

   Plasmid

   #194989

   Purpose

   overexpress CD63

   Depositor

   Angelique Bordey

   Insert

   GFP-CD63
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMU-PMr

   Plasmid

   #61180

   Purpose

   Fluorescent reporter for plasma membrane AtPIP2a-mCherry expressed under AtUBQ10 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   AtPIP2a
   

   Use

   Tags

   mCherry

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available since

   April 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-HA AKT1

    Plasmid

    #78778

    Purpose

    To overexpress AKT1 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUltra-puro-RTTA3

    Plasmid

    #58750

    Purpose

    silence doxycyclin inducible pUltra-dox

    Depositor

    Kitai Kim, Yildirim Dogan

    Insert

    Reverse TET-Transactivator 3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    loxP5171

    Promoter

    Ubiquitin C promoter

    Available since

    Oct. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMyBADC-kan

    Plasmid

    #84689

    Purpose

    Protein expression in mycobacteria

    Depositor

    Matthias Wilmanns

    Article

    Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    AraC

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pENTR-R2L3-IRES-puro-pA

    Plasmid

    #139529

    Purpose

    Provides IRES-puro-pA cassette on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    puromycin resistant gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEYFP-N1-Pink1

    Plasmid

    #101874

    Purpose

    Transient expression of Pink1-YFP in mammalian cells

    Depositor

    Richard Youle

    Article

    Narendra et al PLoS Biol. 2010 Jan 26;8(1):e1000298. do

    Insert

    PTEN induced putative kinase 1 (PINK1 Human)
    

    Use

    Tags

    fluorescent tag EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLKO.1 mito-mEmerald

    Plasmid

    #174542

    Purpose

    labels mitochondria with mEmerald

    Depositor

    Minna Roh-Johnson

    Article

    Kidwell et al eLife. 2023 Mar 6;12:e85494. doi: 10.755

    Insert

    MTS-mEmerald
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLVX-CMV-Galpha-i1 ONE-GO

    Plasmid

    #189735

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Inserts

    Galpha-i1 ONE-GO
    GNAI1 (Gnai1 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV CEPIA3mt

    Plasmid

    #58219

    Purpose

    Green fluorescent indicator for calcium imaging in the mitochondria (moderately low affinity variant)

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    CEPIA3mt
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    GCaMP2 N263Y E282V E334D M339L

    Promoter

    CMV

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRecT_2

    Plasmid

    #167546

    Purpose

    Encodes an IPTG inducible recT from E. faecium Com15 used to ectopically express recT in Enterococcus for recombineering.

    Depositor

    Howard Hang

    Article

    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

    Insert

    E. faecium Com15 recT under an IPTG inducible promoter, with lacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    IPTG

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti 4.1 Ex miR200c-141

    Plasmid

    #35534

    Purpose

    3rd generation lentiviral vector

    Depositor

    Greg Goodall

    Article

    Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

    Insert

    miR200c-141 genomic cluster (MIR200C, MIR141 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIZ-Flag6His-Siwi

    Plasmid

    #50560

    Purpose

    Expresses FLAG, His tagged Siwi in silkworm cell line BmN4 or insect cells

    Depositor

    Yukihide Tomari

    Article

    Kawaoka et al RNA. 2009 Jul;15(7):1258-64. doi: 10.126

    Insert

    Siwi (Aubergine )
    

    Use

    Tags

    6xHIS and FLAG

    Expression

    Insect

    Mutation

    Promoter

    OpIE2

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge