We narrowed to 22,696 results for: Sis;

1. pEPQD1CB0109

   Plasmid

   #177042

   Purpose

   MoClo Level 1, position 2, transcriptional unit for transient expression of geranyl pyrophosphate synthase (PaGPPS1) from Picea abies driven by 35S promoter; contains a chloroplast transit peptide

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   geranyl pyrophosphate synthase (PaGPPS1) from Picea abies
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLBT0160

   Plasmid

   #172812

   Purpose

   Level 1 module containing P19_P1 F

   Depositor

   Benjamin Field

   Article

   Velay et al Plant Methods. 2022 May 26;18(1):69. doi

   Insert

   p35S-5UTMV-CDS1_P19-3U+TERNOS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAsCpf1GG

   Plasmid

   #131011

   Purpose

   Backbone plasmid for generating CRISPR arrays for AsCas12a. Contains a GFP-dropout cassette and a direct repeat.

   Depositor

   Chase Beisel

   Article

   Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

   Inserts

   direct repeat of AsCas12a
   GFP expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUPD2 AtUBQ10 (GB2478)

   Plasmid

   #160594

   Purpose

   UBQ10 promoter from A. thaliana for constitutive expression in plant systems.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   AtUBQ10
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGWB526

   Plasmid

   #74868

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with T7 (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available Since

   July 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA6 MCV LTco S147A

   Plasmid

   #112192

   Purpose

   Expression of MCPyV LT mutant (Ser 147 to Ala)

   Depositor

   Patrick Moore

   Article

   Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

   Insert

   MCPyV LT codon optimized
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. SaKKH-ABE8e-dimer

   Plasmid

   #138503

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(WT)-ecTadA(8e)-nSaCas9 (KKH)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. NSSCP

   Plasmid

   #183190

   Purpose

   mRFP-PopZ expressed under J23116 promoter; split N terminal of TEV protease-SpmXΔC expressed under Tac promoter; SpmXΔC-split C terminal of TEV protease expressed under Tac promoter; DivIVA-sfGFP-TEV

   Depositor

   Hsiao-Chun Huang

   Article

   Hong et al ACS Synth Biol. 2021 Aug 20;10(8):1830-1

   Inserts

   mRFP-PopZ
   DivIVA-GFP(tevR-50)
   SpmX-TEV(C)
   TEV(N)-SpmX
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV-miRE_shBRD9_508-SV40-GFP

   Plasmid

   #75138

   Purpose

   Retroviral expression plasmid encoding shBRD9_508 (targeting human BRD)

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   shBRD9_508
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 23, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pCIneo-HA-Ago2

    Plasmid

    #115362

    Purpose

    Expression vector to produce HA-tagged human Ago2 protein without N-peptide

    Depositor

    Witold Filipowicz, Ramesh Pillai

    Article

    Pillai et al RNA. 2004 Oct . 10(10):1518-25.

    Insert

    Argonaute 2 (AGO2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDF1-ERBB4 (E542K)

    Plasmid

    #29539

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

    Insert

    ERBB4 (ERBB4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pBCC155-MXMT

    Plasmid

    #79816

    Purpose

    CFP-based BiFC vector for plants (Positive control: MXMT)

    Depositor

    Masamitsu Wada, Yutaka Kodama

    Article

    Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

    Insert

    CaMXMT1
    

    Use

    Reporter plasmid

    Tags

    CC155/CFP (155–239)

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S promoter and CaMV35S

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5/FRT/TO GFP DNAJB7

    Plasmid

    #19503

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB7 (DNAJB7 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMIR-D10 mutUTR

    Plasmid

    #19116

    Depositor

    Bob Weinberg

    Article

    Ma et al Nature. 2007 Oct 11. 449(7163):682-8.

    Insert

    HOXD10 mut 3' UTR (HOXD10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

15. LbABE8e-dimer

    Plasmid

    #138505

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(WT)-ecTadA(8e)-dLbCas12a
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAimR

    Plasmid

    #98687

    Purpose

    Expresses AimR gene from the B. subtilis phage phi3T, with its native promoter, C' His-tag

    Depositor

    Rotem Sorek

    Article

    Erez et al Nature. 2017 Jan 26;541(7638):488-493. d

    Insert

    AimR
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Native

    Available Since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJMP1219

    Plasmid

    #119259

    Purpose

    rfp "test" strain

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available Since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSuper mouse DGCR8-3

    Plasmid

    #14774

    Depositor

    Tyler Jacks

    Article

    Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

    Insert

    DGCR8 shRNA (Dgcr8 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pAc-­His-­zfTpo

    Plasmid

    #64309

    Purpose

    Express soluble His-tagged zebrafish thrombopoietin in insect cells

    Depositor

    Petr Bartunek

    Article

    Svoboda et al Blood. 2014 Jul 10;124(2):220-8. doi: 10

    Insert

    Thrombopoietin (thpo Zebrafish)
    

    Use

    Tags

    (His)6

    Expression

    Insect

    Mutation

    Thrombopoietin starts at AA37 (AA1-36 encode the …

    Promoter

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pXylA-RgTALsyn

    Plasmid

    #100948

    Purpose

    Expression of Tyrosine Ammonia Lyase from constitutive XylA promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

    Insert

    RgTALsyn
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Silent mutation to remove internal NdeI site on T…

    Promoter

    XylA (Constitutive)

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDGE663

    Plasmid

    #153261

    Purpose

    M6E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDGE665

    Plasmid

    #153263

    Purpose

    M8E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDGE654

    Plasmid

    #153252

    Purpose

    M1E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRSET FLIPglu-600uDelta11 Aphrodite

    Plasmid

    #13569

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16A Delta11 Aphrodite
    

    Use

    Tags

    Aphrodite and ECFP

    Expression

    Bacterial

    Mutation

    Fluorophore-derived linker shortened. F16A mutati…

    Promoter

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pVMG0146_Cq-U6-1_2xBbsI-gRNA

    Plasmid

    #169238

    Purpose

    Expression of gRNA in Culex quinquefasciatus or other mosquitoes

    Depositor

    Valentino Gantz

    Article

    Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

    Insert

    Cq-U6-1_2xBbsI-gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    CPIJ039653

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pVMG0149_Cq-U6-6_2xBbsI-gRNA

    Plasmid

    #169323

    Purpose

    Expression of gRNA in Culex quinquefasciatus or other mosquitoes

    Depositor

    Valentino Gantz

    Article

    Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

    Insert

    pVMG0149_Cq-U6-6_2xBbsI-gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    CPIJ039596

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLL3.7 shRNA against CRTC1

    Plasmid

    #72710

    Purpose

    Lentiviral expression of shRNA against CRTC1 (3rd generation)

    Depositor

    Freddy Jeanneteau

    Article

    Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

    Insert

    CRTC1
    

    Use

    Lentiviral

    Tags

    CMV-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

28. pEF1α-spider-monkey-retroCHMP3-HA

    Plasmid

    #154179

    Purpose

    expresses spider monkey retroCHMP3 in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    retroCHMP3
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. LentiU6-mHbb-bh1_ gRNA_1-MS2-Puro

    Plasmid

    #192681

    Purpose

    Lentiviral expression of sgRNA targeting mHbb-bh1promoter to activate mouse Hbb-bh1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Mouse Hbb-bh1 activating gRNA #1 (Hbb-bh1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDESTFLAGHApuro3-Ago2K533AK570A

    Plasmid

    #14104

    Depositor

    Thomas Tuschl

    Article

    Ma et al Nature. 2005 Mar 31. 434(7033):666-70.

    Insert

    Ago2 (AGO2 Human)
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    changed Lys533 to Ala, Lys570 to Alanine

    Promoter

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only