We narrowed to 7,458 results for: pac

1. pPBbsr-6080NES(Epac-sensor)

   Plasmid

   #162487

   Purpose

   hyBRET-Epac, an Epac biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

   Insert

   Epac-sensor (RAPGEF3 Human, Synthetic)
   

   Use

   Tags

   YPet and Turquoise2GL

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc5.1B-lambdaN-HA-DmeIF4G-V5His6

   Plasmid

   #79259

   Purpose

   Expresses lambdaN-HA-tagged and C-terminal V5-His6-tagged DmeIF4G in S2 cells

   Depositor

   Elisa Izaurralde

   Article

   Zekri et al EMBO J. 2013 Apr 3;32(7):1052-65. doi: 1

   Insert

   DmeIF4G (eIF4G1 Fly)
   

   Use

   Schneider 2 cell expression

   Tags

   V5-His6 and lambdaN-HA

   Expression

   Mutation

   Promoter

   Ac5 promoter

   Available since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CaMKII-pAceR-Kv2.1PR

   Plasmid

   #195531

   Purpose

   Red fluorescent, positive response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

   Depositor

   Vincent Pieribone

   Article

   Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

   Insert

   pAceR-Kv2.1 proximal restriction sequence
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   VARNAM 78E, 81D, 92N; SI linker

   Promoter

   CaMKII

   Available since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAcGFP-Hyg-CTCF-Nterm-N1

   Plasmid

   #131800

   Purpose

   Expresses the [N-terminus of human CTCF (a.a. 2-265)]-GFP in mammalian cells

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   CTCF (CTCF Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 796-2181 (the N-terminus is l…

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAcGFP-Hyg-CTCF-Cterm-N1

   Plasmid

   #131801

   Purpose

   Expresses the [C-terminus of human CTCF (a.a. 577-728)]-GFP in mammalian cells

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   CTCF (CTCF Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-1731

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Sec61 beta pAcGFP-C1 (802)

   Plasmid

   #62008

   Purpose

   mammalian expression of GFP-Sec61 beta

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   Sec61 beta (SEC61B Human)
   

   Use

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pAC5-dual-dCas9VP48-sgTetO

   Plasmid

   #48237

   Purpose

   Dual expression construct expressing both dCas9VP48 and sgTetO from separate promoters

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9VP48 and sgTetO
   

   Use

   CRISPR

   Tags

   HA-tag and VP48

   Expression

   Mammalian

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   Available since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. sgRNA scaffold-spacer-human U6

   Plasmid

   #214697

   Purpose

   provide "sgRNA scaffold-spacer-human U6" fragment

   Depositor

   Yin Shen

   Article

   Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

   Insert

   sgRNA scaffold-spacer-human U6
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX459-HypaCas9-mR2-ANLN_sgRNA

   Plasmid

   #183876

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ANLN sgRNA for N-terminal tagging of anillin in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ANLN sgRNA spacer (ANLN Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAc5.1B-EGFP-DmGIGYF_641-1574_AH

    Plasmid

    #148920

    Purpose

    Insect Expression of DmGIGYF_641-1574

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF_641-1574 (Gyf Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAc5.1B-EGFP-DmGIGYF_1-640_AH

    Plasmid

    #148919

    Purpose

    Insect Expression of DmGIGYF_1-640

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF_1-640 (Gyf Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAcGP67A-rCL1-HormR-GAIN

    Plasmid

    #37847

    Depositor

    Axel Brunger

    Article

    Arac et al EMBO J. 2012 Feb 14;31(6):1364-78. doi:

    Insert

    CL1 (Adgrl1 Rat)
    

    Use

    Baculovirus transfer vectors

    Tags

    8xHIS

    Expression

    Mutation

    HormR and GAIN domains only (aa460–849

    Promoter

    Polyhedrin

    Available since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pACP-GPI Control Plasmid

    Plasmid

    #101131

    Purpose

    Expression of ACP-Tagged GPI anchor in Mammalian cells

    Depositor

    Ana Egana, New England Biolabs

    Insert

    GPI
    

    Use

    Tags

    ACP-Tag (ACP wt)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pFGC5941-PacI-ath-STTM2937

    Plasmid

    #84112

    Purpose

    target miRNA2937 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM2937
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pACP-ADRβ2 Control Plasmid

    Plasmid

    #101128

    Purpose

    Expression of ACP-Tagged β2 Adrenergic Receptor in Mammalian cells

    Depositor

    Ana Egana, New England Biolabs

    Insert

    Beta-2-Adrenergic Receptor (ADRB2 Human)
    

    Use

    Tags

    ACP-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pX459-HypaCas9-mR2-ACTB_sgRNA

    Plasmid

    #183884

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ACTB sgRNA for N-terminal tagging of beta-actin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ACTB sgRNA spacer (ACTB Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pX459-HypaCas9-mR2-TUBA1B_sgRNA

    Plasmid

    #183888

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    TUBA1B sgRNA spacer (TUBA1B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX459-HypaCas9-mR2-RHOA_sgRNA

    Plasmid

    #183877

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and RHOA sgRNA for N-terminal tagging of RhoA in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    RHOA sgRNA spacer (RHOA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFGC5941-PacI-ath-STTM159

    Plasmid

    #84084

    Purpose

    target miRNA159 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM159
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAc-sgRNA-Cas9.scr_2

    Plasmid

    #190704

    Purpose

    Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Scrambled sgRNA 2
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila U6

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFGC5941DR5-PacI-ath-STTM390

    Plasmid

    #84117

    Purpose

    target miRNA390 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM390
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFGC5941-PacI-ath-STTM858a/b

    Plasmid

    #84177

    Purpose

    target miRNA858 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM858a/b
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAC8-TwinStrep-3C-DrHDAC6 (40-831)

    Plasmid

    #180226

    Purpose

    Baculovirus transfer plasmid to express TwinStrep-3C-DrHDAC6 (40-831)

    Depositor

    Patrick Matthias

    Article

    Langousis et al Methods Mol Biol. 2023;2589:467-480. doi

    Insert

    Histone deacetylase 6 (hdac6 Zebrafish)
    

    Use

    Tags

    TwinStrep-3C

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRK5-H1-shRNA-CMV-HA-PACSIN1

    Plasmid

    #72577

    Purpose

    Bicistronic construct expressing PACSIN1 shRNA and HA-PACSIN1-rescue construct

    Depositor

    Richard Huganir

    Article

    Anggono et al Proc Natl Acad Sci U S A. 2013 Aug 20;11

    Inserts

    PACSIN1 (Pacsin1 Mouse)
    H1-PACSIN1 shRNA cassette
    

    Use

    RNAi

    Tags

    HA

    Expression

    Mammalian

    Mutation

    5 silent mutations around shRNA recognition seque…

    Promoter

    CMV and H1

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFGC5941-PacI-ath-STTM828

    Plasmid

    #84157

    Purpose

    target miRNA828 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM828
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

26. pAC-U63-tgRNA-nlsBFP

    Plasmid

    #169029

    Purpose

    gRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-mTagBFP-NLS marker.

    Depositor

    Chun Han

    Article

    Allen et al PLoS Biol. 2021 Jan 14;19(1):e3001061. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6:3

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAC-EsaR-D91G-EsaI

    Plasmid

    #47662

    Purpose

    esaI gene downstream of esaR-a272g in pAC-EsaR-D91G (CmR)

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Synth Biol. 2013 Jul 23.

    Insert

    esaI-term
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Sept. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

28. pFGC5941-PacI-ath-STTM843

    Plasmid

    #84169

    Purpose

    target miRNA843 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM843
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFGC5941-PacI-ath-STTM845

    Plasmid

    #84170

    Purpose

    target miRNA845 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM845
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAGGS-pac-T2A-iCre

    Plasmid

    #113849

    Purpose

    Puromycin resistance gene and iCre recombinase

    Depositor

    Mario Capecchi

    Insert

    pac-T2A-iCre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge