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Showing: 1801 - 1850 of 8993 results

  1. pCIFR-6

    Plasmid
    #227665
    Purpose
    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Gm) used for selecting for the integration can be removed in a later stage with pFNC.
    Depositor
    Pablo Ivan Nikel
    Article
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Insert
    msfGFP
    Expression
    Bacterial
    Promoter
    p14G-BCD2
    Available Since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    227665-plasmid-map-sequence-id-458898

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  2. pCIFR-4

    Plasmid
    #227664
    Purpose
    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Sm) used for selecting for the integration can be removed in a later stage with pFNC.
    Depositor
    Pablo Ivan Nikel
    Article
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Insert
    msfGFP
    Expression
    Bacterial
    Promoter
    p14G-BCD2
    Available Since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    227664-plasmid-map-sequence-id-458897

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  3. pCIFR-2

    Plasmid
    #227663
    Purpose
    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Km) used for selecting for the integration can be removed in a later stage with pFNC.
    Depositor
    Pablo Ivan Nikel
    Article
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Insert
    msfGFP
    Expression
    Bacterial
    Promoter
    p14G-BCD2
    Available Since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    227663-plasmid-map-sequence-id-458896

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  4. pFNC-6

    Plasmid
    #227669
    Purpose
    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. GmR, easily curable via sucrose counterselection.
    Depositor
    Pablo Ivan Nikel
    Article
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Inserts
    BxbI phage integrase
    sacB
    Expression
    Bacterial
    Promoter
    PEM7
    Available Since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    227669-plasmid-map-sequence-id-458626

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  5. ATP1A1_T804N_hPGK1_mScarlet-I-TOSI_Donor

    Plasmid
    #173208
    Purpose
    HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the mScarlet-I mTOR signaling indicator (TOSI) cassette to ATP1A1 intron 17.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1-T804N mScarlet-I-TOSI donor
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    hPGK1
    Available Since
    April 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    173208-plasmid-map-sequence-id-339517

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  6. KG#797

    Plasmid
    #110916
    Purpose
    Expresses the active zone - enriched protein Sentryn-GFP in a subset of cholinergic ventral nerve cord motor neurons
    Depositor
    Kenneth Miller
    Article
    Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:
    Inserts
    unc-129 promoter
    STRN-1 (Sentryn)-GFP
    unc-54 3' control region with 1 intron just upstream and 1 intron in control region
    Tags
    GFP (contains 3 artificial introns)
    Expression
    Bacterial
    Available Since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    110916-plasmid-map-sequence-id-212580

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  7. CMV-10xPP7-Qb model based

    Plasmid
    #158203
    Purpose
    Cassette of 10 different binding sites with high affinity to both PP7 and Qb phage coats protein, as computed by the ML model.
    Depositor
    Roee Amit
    Article
    Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:
    Insert
    10 binding sites with high affinity to PP7 and QB coat proteins based on the machine-learning model
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    158203-plasmid-map-sequence-id-311815

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  8. pGS1613

    Plasmid
    #63789
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal tDimer2 Strep-tag II followed by HIS5 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    tDimer2-Strep-tag II followed by HIS5
    Use
    E. coli cloning vector
    Tags
    tDimer2-Strep-tag II
    Promoter
    none
    Available Since
    April 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    63789-plasmid-map-sequence-id-110438

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  9. pGS1122

    Plasmid
    #63790
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eSapphire Twin-Strep tag followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eSapphire-Twin-Strep tag followed by URA3
    Use
    E. coli cloning vector
    Tags
    eSapphire-Twin-Strep tag
    Promoter
    none
    Available Since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    63790-plasmid-map-sequence-id-106477

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  10. pGS1412

    Plasmid
    #63787
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCitrine Strep-tag II followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eCitrine-Strep-tag II followed by URA3 selection marker
    Use
    E. coli cloning vector
    Tags
    eCitrine-Strep-tag II
    Promoter
    none
    Available Since
    April 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    63787-plasmid-map-sequence-id-106472

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  12. pCdOpt-BMX

    Plasmid
    #203929
    Purpose
    This plasmid contains a codon optimized BleMX drug resistance cassette for generating gene deletions in C. glabrata, C. auris, C. albicans or S. cerevisiae
    Depositor
    Scott Briggs
    Article
    Gregor et al mSphere. 2023 Nov 6:e0031123. doi: 10.11
    Insert
    BleMX
    Expression
    Yeast
    Mutation
    BleMX was codon optimized for Candida albicans or…
    Available Since
    Dec. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    203929-plasmid-map-sequence-id-414816

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  13. pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)

    Plasmid
    #47868
    Purpose
    This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.
    Depositor
    Erik Sontheimer, James Thomson
    Article
    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.
    Inserts
    NmCas9
    U6pr-tracrRNA
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    EF1a and U6
    Available Since
    Sept. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    47868-plasmid-map-sequence-id-68833

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  14. pGLTR-X-GFP

    Plasmid
    #58245
    Purpose
    GATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and eGFP
    Depositor
    Stephan Geley
    Article
    Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:
    Inserts
    attR1-Cm(R)-CcdB-attR2
    TetR-NLS-T2A-GFP
    Use
    Lentiviral and RNAi; Gateway destionation vector
    Tags
    GFP, NLS, T2A, and TetR
    Expression
    Mammalian
    Promoter
    SFFV and no
    Available Since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    58245-plasmid-map-sequence-id-89669

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  15. Transposon-for RMCE_Sleeping Beauty_LIR-CAG-Frt-red(katushka)-P2A-Puro-F3-PA-RIR

    Plasmid
    #67275
    Purpose
    For sleeping beauty transposition and establishment of an recombinase mediated cassette exchange docking site. To make RMCE in cell line instead of flp in cell lines
    Depositor
    Jannik Elverløv-Jakobsen
    Article
    Callesen et al PLoS One. 2016 Aug 19;11(8):e0161471. do
    Insert
    puromycin resistant gene
    Tags
    linked to red flouresent protein gene (katushka)
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    July 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    67275-plasmid-map-sequence-id-118496

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  20. pQCXIP GFP-MOSPD2 RD/LD

    Plasmid
    #186468
    Purpose
    Expression of human MOSPD2 (mutant R404D/L406D, unable to bind FFAT motifs) fused to EGFP in mammalian cells
    Depositor
    Catherine Tomasetto
    Article
    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017
    Insert
    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    R404D/L406D (mutant unable to bind FFAT motifs)
    Available Since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    186468-plasmid-map-sequence-id-366060

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  22. pGLTR-S-PURO

    Plasmid
    #58244
    Purpose
    GATEWAY-compatible lentiviral RNAi delivery vector; SFFV-driven Puromycin resistance
    Depositor
    Stephan Geley
    Article
    Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:
    Inserts
    attR1-Cm(R)-CcdB-attR2
    Puro(R)
    Use
    Lentiviral and RNAi; Gateway destination vector
    Expression
    Mammalian
    Promoter
    SFFV and no
    Available Since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    58244-plasmid-map-sequence-id-89655

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  23. pDONR P2R-P3-V5-6xHis

    Plasmid
    #48347
    Purpose
    Contributes the coding sequence of a V5-6xHis epitope tags cassette as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.
    Depositor
    Anna Planas, Tomas Santalucia
    Article
    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:
    Insert
    V5 and 6xHis epitope tags cassette
    Use
    Gateway entry clone
    Mutation
    Contains a stop codon at the end of the epitope t…
    Promoter
    none
    Available Since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    48347-plasmid-map-sequence-id-70558

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  27. CMV-10xMS2 no wild type

    Plasmid
    #158204
    Purpose
    Cassette of 10 different binding sites with high affinity to MS2 phage coat protein, as extracted form the oligo pool experiment. It does not contain the MS2-wt sequence, therefore QCP cannot bind it.
    Depositor
    Roee Amit
    Article
    Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:
    Insert
    10 binding sites of MS2 without the WT sequence from oligo library experiment
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    158204-plasmid-map-sequence-id-311816

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  28. CMV-10xQb no wild type

    Plasmid
    #158205
    Purpose
    Cassette of 10 different binding sites with high affinity to Qb phage coat protein, as extracted form the oligo pool experiment. It does not contain the Qb-wt sequence, therefore MCP cannot bind it.
    Depositor
    Roee Amit
    Article
    Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:
    Insert
    10 binding sites of QB coat protein without the WT sequence based on the oligo library experiment
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    158205-plasmid-map-sequence-id-311817

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  32. pXPR_219

    Plasmid
    #164559
    Purpose
    Lentiviral vector that encodes two sgRNA expression cassettes. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Article
    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s
    Inserts
    First sgRNA cassette
    Second sgRNA cassette
    Use
    CRISPR and Lentiviral; Dual sgrna
    Expression
    Mammalian
    Promoter
    Human U6 and Mouse U6
    Available Since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    164559-plasmid-map-sequence-id-330205

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  34. pLenti_mpx_AsCas12a(dual-gRNA)_h7SK(PacI-ClaI)_U6(I-SceI-NheI)_PGK-puro

    Plasmid
    #189635
    Purpose
    Lentiviral expression of double dual-AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries
    Depositor
    Manuel Kaulich
    Article
    Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.
    Insert
    h7SK arrayed Cas12a gRNA cassette, hU6 arrayed Cas12a gRNA cassette, PGK puro cassette
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Nov. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    189635-plasmid-map-sequence-id-414905

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  35. pET15b MOSPD2 RD/LD [282-490]

    Plasmid
    #186470
    Purpose
    Expression of human MOSPD2 (MSP domain from residue 282 to 490; mutant R404D/L406D, unable to bind FFAT motifs), fused to a 6HIS tag, in bacteria
    Depositor
    Catherine Tomasetto
    Article
    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017
    Insert
    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    Tags
    6HIS
    Expression
    Bacterial
    Mutation
    MSP domain [282-490]
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    186470-plasmid-map-sequence-id-367335

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  36. pGG431

    Plasmid
    #165615
    Purpose
    Vector for expression of SpCas9 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-CYC1t (KanR cassette in place of the PID)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    TEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID)
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    Corrected homology relative to wild type SpCas9 (…
    Promoter
    TEF1
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165615-plasmid-map-sequence-id-330960

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  39. pAAV-hSyn-DIO {ChETA-mRuby2}on-W3SL

    Plasmid
    #111389
    Purpose
    AAV vector with hSynapsin promoter, Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)
    Depositor
    Adam Kepecs
    Article
    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi
    Inserts
    ChETA-mRuby2
    W3SL
    Use
    AAV
    Tags
    mRuby2
    Expression
    Mammalian
    Mutation
    W3SL is built from a shortened WPRE, an upstream …
    Promoter
    hSynapsin
    Available Since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    111389-plasmid-map-sequence-id-212217

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  44. pCBS-4559

    Plasmid
    #249331
    Purpose
    Expresses EPEC DarT2(G49D)-nSpCas9(D10A) in mammalian cells and gfp-dropout cassette for cloning sgRNAs
    Depositor
    Chase Beisel
    Article
    Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038
    Insert
    EPEC DarT2(G49D)-nSpCas9(D10A)
    Tags
    3X Flag tag
    Expression
    Mammalian
    Mutation
    DarT2(G49D) and nSpCas9(D10A)
    Available Since
    Jan. 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    249331-plasmid-map-sequence-id-497004

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  45. pXPR_220

    Plasmid
    #164560
    Purpose
    Lentiviral vector that encodes two sgRNA expression cassettes (one constitutive, one inducible via Flp). Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Article
    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s
    Inserts
    First sgRNA cassette
    Second sgRNA cassette
    Use
    CRISPR and Lentiviral; Flp/frt
    Expression
    Mammalian
    Promoter
    Human U6 and Mouse U6
    Available Since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    164560-plasmid-map-sequence-id-330240

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  49. TFORF1201

    Plasmid
    #142751
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SETDB1 (SETDB1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    142751-plasmid-map-sequence-id-462894

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  50. TFORF1202

    Plasmid
    #144088
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SETDB1 (SETDB1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    144088-plasmid-map-sequence-id-396209

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Showing: 1801 - 1850 of 8993 results