We narrowed to 13,942 results for: cas9

1. pBbdCas9S_P0526-sgRNA500

   Plasmid

   #149641

   Purpose

   parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   PflaB, PpQE30, P0526

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBbdCas9S(RBSmut)

   Plasmid

   #149574

   Purpose

   parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   PflaB, PpQE30

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBbdCas9S(-10TC)

   Plasmid

   #149581

   Purpose

   parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   PflaB, PpQE30

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAtCas9-dead_2

   Plasmid

   #91166

   Purpose

   Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:npt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   AtCas9_D10A+H840A (dead)
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A, H840A

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. p426_Cas9_gRNA-R-ARS607c

   Plasmid

   #87409

   Purpose

   p426_Cas9_gRNA-ARS607c without ribozyme All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS607c sequence CTATTTTTGCTTTCTGCACA in yeast chromosome 6.

   Depositor

   Aindrila Mukhopadhyay

   Article

   Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

   Insert

   Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS607c
   

   Use

   CRISPR

   Promoter

   ADH1, pTyrosine

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pdCas9-M-3F2

   Plasmid

   #73428

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3F2.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor C4 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available Since

   April 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTaCas9-H840A_6

   Plasmid

   #91173

   Purpose

   Cas9 only expression, Cas9 Type: TaCas9_H840A (nickase), Plant Selection: PvUbi2:bar

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TaCas9_H840A (nickase)
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   H840A

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAtCas9-H840A_1

   Plasmid

   #91162

   Purpose

   Cas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   AtCas9_H840A (nickase)
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   H840A

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. peSpdCas9-J23109

   Plasmid

   #113153

   Purpose

   Plasmid constitutively expressing eSpdCas9 protein

   Depositor

   Chong Zhang

   Article

   Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

   Insert

   eSpdCas9
   

   Expression

   Bacterial

   Mutation

   D10A & K810A & H840A & K1003A & R…

   Available Since

   Dec. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330A_dCas9-1x6

    Plasmid

    #63600

    Purpose

    Expresses dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    humanized S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D1361Y

    Promoter

    CBh

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. phumanCas9 (GB0575)

    Plasmid

    #68206

    Purpose

    Provides the human codon optimized CDS of Cas9 protein as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Cas9 coding region (human codon optimised)
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed; human codon optimis…

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBbdCas9S_Psyn-sgRNAflaB

    Plasmid

    #149642

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi flaB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAflaB
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30, Psyn

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAtCas9-dead_1

    Plasmid

    #91165

    Purpose

    Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_D10A+H840A (dead)
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A, H840A

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBbdCas9S_Psyn-sgRNAmreB

    Plasmid

    #149653

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi mreB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAmreB
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30, Psyn

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHA

    Plasmid

    #136659

    Purpose

    Expresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD9:TH1477 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBluescript_VEGFA-TS3_SaCas9_sgRNA2

    Plasmid

    #107330

    Purpose

    U6 driven SaCas9 sgRNA expression for VEGFA site 3

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    VEGFA-TS3 sgRNA
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pICH47742:PtFCP:Cas9YFP

    Plasmid

    #104894

    Purpose

    Cas9, with FCP promoter/terminator, Golden Gate Assembly, for Phaeodactylum transformation

    Depositor

    Thomas Mock

    Article

    Graff van Creveld et al Front Microbiol. 2021 Sep 8;12:688199. d

    Insert

    Cas9
    

    Use

    CRISPR; ; golden gate assembly

    Tags

    YFP

    Available Since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAtCas9-H840A_2

    Plasmid

    #91163

    Purpose

    Cas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_H840A (nickase)
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    H840A

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. KA2706_eSpCas9-PGKPacdtkCh

    Plasmid

    #124204

    Purpose

    Expression of eSpCas9(1.1) and sgRNA

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. UAS-u(XL)Cas9

    Plasmid

    #127386

    Purpose

    Expresses Cas9 at very low level

    Depositor

    Michael Boutros

    Article

    Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

    Insert

    uORF(XL)-Cas9
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    UAST

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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