165,335 results

1. tet-TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA

   Plasmid

   #221235

   Purpose

   Tet-inducible lentiviral HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA
   

   Use

   Lentiviral

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHJL1

   Plasmid

   #199376

   Purpose

   Encodes dual cistronic cytosolic GCaMP6s and mScarlet-I optimized for C. elegans expression

   Depositor

   Hang Lu

   Insert

   GCaMP6s::SL2::mScarlet-I
   

   Expression

   Bacterial

   Mutation

   no mutations

   Available Since

   April 24, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pLenti-EML4-ALK variant 3a I1171N/D1203N

   Plasmid

   #183833

   Purpose

   Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N/D1203N mutation in mammalian cells

   Depositor

   Aaron Hata

   Article

   Shiba-Ishii et al Nat Cancer. 2022 Jun 20. pii: 10.1038/s4

   Inserts

   ALK Receptor Tyrosine Kinase (ALK Human)
   EMAP Like 4 (EML4 Human)
   

   Use

   Lentiviral

   Tags

   The fusion protein of EML4 exon 1-6a and ALK exon…

   Expression

   Mammalian

   Mutation

   I1171N/D1203N

   Promoter

   CMV

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA GFP10-ZIP-GFP11

   Plasmid

   #182243

   Purpose

   Tripartite split-GFP mammalian. Express leucine zipper with GFP10 and GFP11 (self-assembing with GFP1-9)

   Depositor

   Stéphanie Cabantous

   Article

   Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

   Insert

   GFP10-ZIP-11
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLBS-mScarlet

   Plasmid

   #129337

   Purpose

   cytosol marker, lentivirus

   Depositor

   Yasushi Okada

   Article

   Yaginuma et al bioRxiv 2021.10.08.463131

   Insert

   mScarlet (Addgene 85042)
   

   Use

   Lentiviral

   Available Since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV_3XHA-ATP6V1B2-mNeonGreen

   Plasmid

   #228865

   Purpose

   Stable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA and C-terminal mNeonGreen

   Depositor

   Wilhelm Palm

   Article

   Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

   Insert

   ATP6V1B2 (ATP6V1B2 Human)
   

   Use

   Lentiviral

   Tags

   3xHA and mNeonGreen

   Available Since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG:GPI-GFP

   Plasmid

   #32601

   Depositor

   Anna-Katerina Hadjantonakis

   Article

   Rhee et al Genesis. 2006 Apr;44(4):202-18.

   Insert

   GPI-GFP
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL003

   Plasmid

   #215335

   Purpose

   amyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank promoter)

   Depositor

   Gene-Wei Li

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   pSpank

   Available Since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET22b-T7-6h-GST-α-Synuclein

   Plasmid

   #225224

   Purpose

   Alpha synuclein gene fused with gst gene under t7 promoter for bacterial expression of alpha synuclein protein.

   Depositor

   Urartu Seker

   Article

   Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360

   Inserts

   GST
   α-Synuclein
   

   Tags

   6xHis Tag and TEV Cleavage Site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP-PIK3CD

    Plasmid

    #174190

    Purpose

    Expresses PIK3CD in mammalian cells

    Depositor

    Cheng-han Yu

    Article

    Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

    Insert

    PIK3CD (PIK3CD Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. AcGFP-MSH3

    Plasmid

    #215122

    Purpose

    Expresses AcGFP-MSH3 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    MSH3 (MSH3 Human)
    

    Use

    Retroviral

    Tags

    AcGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-N1-MITF-A

    Plasmid

    #38132

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

    Insert

    MITF-A (MITF Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted stop codon

    Promoter

    CMV

    Available Since

    July 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-sgRNA

    Plasmid

    #71409

    Purpose

    There is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBI

    Depositor

    David Sabatini, Eric Lander

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET N6his GFP1-9

    Plasmid

    #182240

    Purpose

    Tripartite split-GFP. E. coli vector. Expression of GFP1-9 detector fragment.

    Depositor

    Stéphanie Cabantous

    Article

    Pedelacq et al Methods Mol Biol. 2019;2025:423-437. doi

    Insert

    GFP1-9
    

    Tags

    6HIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.3_SOX2

    Plasmid

    #26817

    Depositor

    Derrick Rossi

    Article

    Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

    Insert

    5'UTR-SOX2-3'UTR (SOX2 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pNL-CEF-BAFFR-CFP

    Plasmid

    #187002

    Purpose

    Expression of human BAFFR-CFP fusion protein

    Depositor

    Hermann Eibel

    Article

    Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

    Insert

    BAFFR (TNFRSF13C Human)
    

    Use

    Lentiviral

    Tags

    CFP

    Available Since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYC-C

    Plasmid

    #207114

    Purpose

    HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus

    Depositor

    Yifan Cheng

    Article

    Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. shYAP1 # 2

    Plasmid

    #42541

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. miniTurbo-His6_pET21a

    Plasmid

    #107178

    Purpose

    expresses His6-tagged miniTurbo in bacteria

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Expression

    Bacterial

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRISPR-PsppA-mCherry

    Plasmid

    #225428

    Purpose

    Plasmid encodes SgRNA, SpCas9 and homologous arms for homologous recombination of mCherry

    Depositor

    Geir Mathiesen

    Article

    Wiull et al Microbiol Spectr. 2025 Feb 4;13(2):e0202

    Insert

    mCherry, Cas9, SgRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PsppA upstream of mCherry and Cas9, P3 upstream o…

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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