165,307 results

1. pT077

   Plasmid

   #137879

   Purpose

   Donor vector for integration into the human AAVS1 safe harbor locus of Doxicycline-inducible KRAB-dCas9-IRES-EGFP

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   TRE3G-KRAB-dCas9-IRES-EGFP-CAG-TetON-NeoR-SA
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-GFAP-EGFP

   Plasmid

   #50473

   Purpose

   EGFP under the control of GFAP promoter

   Depositor

   Bryan Roth

   Insert

   EGFP
   

   Use

   AAV

   Tags

   N/A

   Promoter

   GFAP

   Available Since

   March 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-TetON-Sox2

   Plasmid

   #110280

   Purpose

   Doxycycline-inducible overexpression of Sox2

   Depositor

   Trudy Oliver

   Article

   Mollaoglu et al Immunity. 2018 Oct 16;49(4):764-779.e9.

   Insert

   SRY (sex determining region Y)-box 2 (Sox2 Mouse)
   

   Use

   Lentiviral

   Tags

   No fusion genes

   Expression

   Mammalian

   Mutation

   Wild type

   Promoter

   TRE

   Available Since

   Oct. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-SENP3

   Plasmid

   #34554

   Depositor

   Mary Dasso

   Article

   Yun et al J Cell Biol. 2008 Nov 17;183(4):589-95.

   Insert

   SENP3 (SENP3 Human)
   

   Tags

   EGFP

   Promoter

   CMV

   Available Since

   Dec. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.3_OCT4

   Plasmid

   #26816

   Depositor

   Derrick Rossi

   Article

   Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

   Insert

   5'UTR-OCT4-3'UTR (POU5F1 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 17, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pFUW-tetO-Batf3

   Plasmid

   #139837

   Purpose

   doxycycline-inducible expression of mouse Batf3 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   Batf3 (Batf3 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE-mCMV

   Available Since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-CSF1R-V5/HIS

   Plasmid

   #201984

   Purpose

   expression of human CSF1R receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   CSF1R receptor tyrosine kinase (CSF1R Human)
   

   Tags

   V5, 6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. HIV-dual-GT

   Plasmid

   #122696

   Purpose

   Dual-fluorescence reporter to monitor the expression of HIV-1 early genes and late genes simultaneously.

   Depositor

   Klaus Überla

   Article

   Xiao et al mBio. 2021 Jan 19;12(1). pii: mBio.02525

   Inserts

   mTagBFP2
   DsRed-max
   

   Use

   Lentiviral

   Tags

   PEST domain

   Promoter

   HIV-1 LTR

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSNA

   Plasmid

   #135192

   Purpose

   Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains ampicillin resistance cassette

   Depositor

   Hongqing Fang

   Article

   Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

   Insert

   I-SceI lamba Red
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   None

   Promoter

   araBAD

   Available Since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. p3962 pcDNA3-IRF3-FL

    Plasmid

    #22860

    Depositor

    Peter Howley

    Article

    Kirshner et al J Virol. 2005 Jul . 79(14):9320-4.

    Insert

    IRF3 (IRF3 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. GRM1-DuET

    Plasmid

    #213300

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GRM1 (GRM1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDD143

    Plasmid

    #119880

    Purpose

    Arabinose inducible dCas9sth1 and constitutive sgRNA on pAL5000/pMB1 backbone with gentamicin resistance.

    Depositor

    Tae Seok Moon

    Article

    DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

    Insert

    pBAD_dCas9sth1
    

    Expression

    Bacterial

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-PE2-SpRY-P2A-GFP

    Plasmid

    #159981

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

    Insert

    PE2-SpRY-P2A-GFP
    

    Tags

    BpSV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-T7-SpCas9-NG-P2A-EGFP (RTW3677)

    Plasmid

    #139994

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJW1518

    Plasmid

    #62388

    Purpose

    pFA6a -mCherry-BirA::HIS5

    Depositor

    Jonathan Weissman

    Article

    Williams et al Science. 2014 Nov 7;346(6210):748-51. do

    Insert

    mCherry-BirA
    

    Use

    Yeast c-terminal tagging vector

    Tags

    mCherry-BirA

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSEVA63-GG

    Plasmid

    #208015

    Purpose

    for cloning homology arms, with GFP as counter selection marker

    Depositor

    Pablo Ivan Nikel

    Article

    Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

    Insert

    msfGFP
    

    Promoter

    14G

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. PGK-AAVS1ZFNR

    Plasmid

    #60915

    Purpose

    encodes zinc finger nuclease specific to AAVS1 locus (right)

    Depositor

    Paul Gadue

    Article

    Sim et al Methods Mol Biol. 2015 Jan 29.

    Insert

    AAVS1-ZFNR
    

    Promoter

    PGK

    Available Since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pWM_12x601_15bpLinker

    Plasmid

    #157786

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 15 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_15bp linker
    

    Expression

    Bacterial

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPM47 (UPR-RFP CEN/ARS URA3)

    Plasmid

    #20132

    Depositor

    Feroz Papa

    Article

    Merksamer et al Cell. 2008 Nov 28. 135(5):933-47.

    Insert

    UPR-RFP
    

    Expression

    Yeast

    Available Since

    Feb. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a MDHP_YEAST

    Plasmid

    #204233

    Purpose

    Bacterial expression of yeast peroxisomal malate dehydrogenase ; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDHP_YEAST (MDH3 Budding Yeast)
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    The last 3 C-terminal residues (SKL) are the pero…

    Promoter

    Lac

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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