We narrowed to 17,860 results for: COMP

1. Pdpy-30-miniCAFE

   Plasmid

   #170115

   Purpose

   Dpy-30 promoter-driven expression of a truncated VPR-dCjCas9 fusion protein (MiniCAFE) for activation of gene expression.

   Depositor

   Zhili Rong

   Article

   Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

   Insert

   MiniCAFE
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D8A, HNH-truncation (Δ495–609 aa) in CjCas9

   Promoter

   Dpy-30

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Nsp14D-mCherry

   Plasmid

   #165139

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp14D (ORF1ab codon optimized SARS-COV-2)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. [UBC][EIF4e][GG cloning]

   Plasmid

   #97189

   Purpose

   EIF4e plasmid with Pum cloning site

   Depositor

   Edward Boyden

   Article

   Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Insert

   EIF4E variant 1 (EIF4E Human)
   

   Use

   Synthetic Biology

   Tags

   empty Pum cloning site

   Expression

   Mammalian

   Mutation

   Promoter

   UBC (ubiquitin C)

   Available since

   Nov. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Nsp4 deltaC-EGFP

   Plasmid

   #165126

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp4 deltaC (ORF1ab codon optimized SARS-COV-2)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. HOXB3 (human) FLAG pSP65

   Plasmid

   #8523

   Depositor

   Corey Largman

   Article

   Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

   Insert

   HOXB3 (HOXB3 Human)
   

   Use

   In vitro transcription/translation

   Tags

   FLAG

   Expression

   Bacterial

   Mutation

   Contains 5 additional amino acids before ATG from…

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pEGB 35S:dCas9:Tnos (GB1191)

   Plasmid

   #68223

   Purpose

   Transcriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoter

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed; human codon optimis…

   Promoter

   35S

   Available since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV6-XL4 ASXL1 (p.R404X) 3x FLAG

   Plasmid

   #74263

   Purpose

   Cancer-associated ASXL1 truncation mutant in mammalian expression vector

   Depositor

   Anjana Rao

   Article

   Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

   Insert

   ASXL1 (ASXL1 Human)
   

   Use

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   p.R404X truncation

   Promoter

   CMV

   Available since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pET28-MHL: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

   Plasmid

   #167849

   Purpose

   Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

   Depositor

   Cheryl Arrowsmith

   Article

   Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

   Insert

   nsp10, nsp16
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 22, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pDEST-DHFR F[1,2]-C (TRP1)

   Plasmid

   #177795

   Purpose

   Gateway destination vector to insert genes of interest having a C-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

   Depositor

   Christian Landry

   Article

   Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

   Type

   Empty backbone

   Use

   Tags

   DHFR F[1,2]

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-MBP-Foldon-ATG9 (830-839)

    Plasmid

    #188091

    Purpose

    To make ATG9 C-terminal tail trimer for expression in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 (ATG9A Human)
    

    Use

    Tags

    MBP tag

    Expression

    Mammalian

    Mutation

    ATG9 (830-839)

    Promoter

    CMV

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-6P-1-INTS3-C

    Plasmid

    #128417

    Purpose

    Express INTS3 C-termini (514-1042 aa) in E. coli with a GST tag (N-terminal)

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

    Insert

    INTS3 (INTS3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    INTS3 C-terminal region (514-1042 aa)

    Promoter

    Tac promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEBTet-SNAP-Cep70

    Plasmid

    #136821

    Purpose

    Mammalian expression of the centrosomal protein Cep70 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Cep70 (CEP70 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHH0103_ARHGEF6-1/1

    Plasmid

    #91515

    Purpose

    Protein expression and purification of human SH3 domain construct ARHGEF6-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ARHGEF6-1/1 (ARHGEF6 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST27-GST-ATXN1 FL(92Q)-S776D

    Plasmid

    #21757

    Depositor

    Huda Zoghbi

    Article

    Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 92 Q(Glutamines) and…

    Promoter

    CMV

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pEBTetDBl-CLIP-α-tubulin

    Plasmid

    #136841

    Purpose

    Mammalian expression of the centrosomal protein _-tubulin N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-TUBA (DNMBP Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. APCS-bio-His

    Plasmid

    #53424

    Purpose

    Expresses full-length Serum amyloid P-component precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    APCS (APCS Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEBTetBl-CLIP-Cep78

    Plasmid

    #136876

    Purpose

    Mammalian expression of the centrosomal protein Cep78 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Cep78 (CEP78 Synthetic, Human)
    

    Use

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO2

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. 3xNLS-NLP-cMyc-cMyc enAspCas12a

    Plasmid

    #182127

    Purpose

    pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc enAspCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    3xNLS-NLP-cMyc-cMyc enAspCas12a
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-AuroraB L154G/H250Y

    Plasmid

    #108492

    Purpose

    expression of EGFP-AuroraB Analog sensitive (L154G/H250Y)

    Depositor

    Susanne Lens

    Article

    Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

    Insert

    AuroraB (AURKB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Analog sensitive L154G/H250Y

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLR5-CAG-NLS-tdiRFP670-2A-LBR

    Plasmid

    #82620

    Purpose

    Expresses NLS-tdiRFP-2A-LBR in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

    Insert

    NLS-tdiRFP670-LBR (Lbr Synthetic, Mouse)
    

    Use

    Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pTriEx4-ADCY6-C2 (917-1165 aa)

    Plasmid

    #113904

    Purpose

    His-S tagged cytoplasmic catalytic domain 2 of Adenylate Cyclase 6

    Depositor

    Anjaparavanda Naren

    Article

    Thomas et al J Biol Chem. 2018 Jun 14. pii: RA118.003

    Insert

    Adenylate Cyclase 6 cytoplasmic catalytic domain 2 (ADCY6 Human)
    

    Use

    Tags

    His-S

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Please see Depositor Comments

    Promoter

    T7, CMV

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pTriEx4-ADCY6-C1(306-672 aa)

    Plasmid

    #113903

    Purpose

    His-S tagged cytoplasmic catalytic domain 1 of Adenylate Cyclase 6

    Depositor

    Anjaparavanda Naren

    Article

    Thomas et al J Biol Chem. 2018 Jun 14. pii: RA118.003

    Insert

    Adenylate Cyclase 6 cytoplasmic catalytic domain 1 (ADCY6 Human)
    

    Use

    Tags

    His-S

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    T7, CMV

    Available since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHH0103_CRK-2/2-SV1/2

    Plasmid

    #91319

    Purpose

    Protein expression and purification of human SH3 domain construct CRK-2/2-SV1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    CRK-2/2-SV1/2 (CRK Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. hnRNPA2_190-303

    Plasmid

    #118822

    Purpose

    Bacterial expression of hnRNPA2 truncation (residues 190 to 302).

    Depositor

    Nicolas Fawzi

    Article

    Amaya et al J Biol Chem. 2018 Dec 21;293(51):19522-1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    His tag and TEV

    Expression

    Bacterial

    Mutation

    contains amino acids 190-302

    Promoter

    Available since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCR3-Flag-AuroraB KD (K106R)

    Plasmid

    #108488

    Purpose

    expression of Flag-AuroraB kinase death (K106R)

    Depositor

    Susanne Lens

    Article

    Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

    Insert

    AuroraB (AURKB Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Kinase death K106A

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAG-MBP-Foldon-ATG9 (692-839)

    Plasmid

    #188087

    Purpose

    To make ATG9 C-terminal tail trimer for expression in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG9 (ATG9A Human)
    

    Use

    Tags

    MBP tag

    Expression

    Mammalian

    Mutation

    ATG9 (692-839)

    Promoter

    CMV

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEGFP-N3/hArf5(WT)-EGFP

    Plasmid

    #79534

    Purpose

    Expresses C-terminally EGFP-tagged Arf5(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf5 (ARF5 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-CMV-dSa VP64 Actc1

    Plasmid

    #99691

    Purpose

    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Actc1 promoter, vector allows for activation of mouse Actc1, can be packaged and delivered as AAV

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG) (Actc1 Synthetic, Mouse)
    

    Use

    AAV and CRISPR

    Tags

    VP64

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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29. pHH0103_SRC-1/1-IS2/2

    Plasmid

    #91251

    Purpose

    Protein expression and purification of human SH3 domain construct SRC-1/1-IS2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SRC-1/1-IS2/2 (SRC Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBrain-GFP-TACC3KDP(S558A)-shTACC3

    Plasmid

    #59357

    Purpose

    Expresses GFP-tagged human TACC3 (RNAi-resistant) with S558A substitution and knocks down endogenous TACC3 via shRNA.

    Depositor

    Stephen Royle

    Article

    Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

    Insert

    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    

    Use

    RNAi

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Silent mutations in amino acids 30-33 to confer s…

    Promoter

    CMV

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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