We narrowed to 11,806 results for: nsf

1. pB[U6-3 wex2 PUb-DsRed]

   Plasmid

   #169010

   Purpose

   For germline transformation. Expresses white exon 2 sgRNA and DsRed marker in all cells.

   Depositor

   Max Scott

   Article

   Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

   Insert

   white ex2 sgRNA
   

   Use

   Piggybac

   Expression

   Insect

   Promoter

   Drosophila melanogaster U6:3

   Available Since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-TRE-DIO-hM3Dq-mCherry (Cre-OFF)

   Plasmid

   #166609

   Purpose

   Encodes Cre-inactivated hM3Dq-mCherry under control of the TRE

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   hM3Dq-mCherry
   

   Use

   AAV

   Promoter

   tetracycline response element

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-DD-T6B-MUT-YFP

   Plasmid

   #235144

   Purpose

   Expresses the inducible mutant T6B peptide fused with DHFR and YFP under the human synapsin promoter

   Depositor

   Giordano Lippi

   Article

   Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

   Insert

   DHFR-fused mutant T6B peptide
   

   Use

   AAV

   Mutation

   5 tryptophan residues of T6B-WT mutated

   Promoter

   hSyn

   Available Since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-FLAG-HA-T6B-MUT-YFP

   Plasmid

   #235140

   Purpose

   Expresses the mutant T6B peptide fused with YFP under the human synapsin promoter

   Depositor

   Giordano Lippi

   Article

   Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

   Insert

   mutant T6B peptide
   

   Use

   AAV

   Tags

   FLAG/HA

   Mutation

   5 tryptophan residues of T6B-WT mutated

   Promoter

   hSyn

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJEP300-pAAV-CMV-MCS2-pA

   Plasmid

   #112138

   Purpose

   CMV driven Multi Cloning Site-2

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   N/A
   

   Use

   AAV

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CMV-SpyCas9

   Plasmid

   #113034

   Purpose

   AAV vector for expression of SpyCas9 in mammalian cells

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   SpyCas9
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRedCm

   Plasmid

   #176225

   Purpose

   LambdaRed-expressing plasmid carrying the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

   Insert

   Lambda Red operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PrhaB and PL

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTS98 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-MCS

   Plasmid

   #199351

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TagBFP-P2A-ALFA

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDOE11 Lat52:mCherry transient

   Plasmid

   #124249

   Purpose

   Allows bimolecular fluorescence complementation via transient expression of two proteins in directly transfected pollen tubes.

   Depositor

   Attila Fehér

   Article

   Lajko et al Plant Cell Rep. 2018 Apr;37(4):627-639.

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. 3×Cherry Dam AID Smash

    Plasmid

    #227374

    Purpose

    For inducible expression and cytosolic localization of Dam

    Depositor

    David Pellman

    Article

    Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

    Insert

    Dam DNA-adenine methyltransferase
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Sst22-GFP

    Plasmid

    #172312

    Purpose

    SST interneuron-restricted gene regulatory element GRE22, to drive GFP expression in SST+ interneurons

    Depositor

    Michael Greenberg

    Article

    Hrvatin et al Elife. 2019 Sep 23;8. pii: 48089. doi: 1

    Insert

    Sst22
    

    Use

    AAV

    Promoter

    pB-Globin

    Available Since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. PZac2.1 hSyn1-Lck-GFP

    Plasmid

    #177333

    Purpose

    Expresses GFP on the plasma membrane of neurons via an Lck localization sequence

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    Lck-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Bacterial

    Promoter

    hSyn1

    Available Since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. His_Naa80d in pTYB12

    Plasmid

    #188455

    Purpose

    Express in E. coli human Naa80 delta (actin's N-terminal acetyl transferase) with N-terminal intein-chitin binding domain tag and 6xHis tag.

    Depositor

    Roberto Dominguez

    Article

    Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

    Insert

    Naa80 (NAA80 Human)
    

    Tags

    intein-CBD, 6xHis

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Ef1a-DIO-OMOR-eYFP

    Plasmid

    #66847

    Purpose

    Expresses Opto-MOR in a Cre dependent manner

    Depositor

    Michael Bruchas

    Article

    Siuda et al Neuron. 2015 May 20;86(4):923-35. doi: 1

    Insert

    Opto Mu opioid receptor
    

    Use

    AAV and Cre/Lox

    Tags

    eYFP

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. L2_UBE2:eG-Lt-CsA

    Plasmid

    #136134

    Purpose

    Plasmid to transform Mp with constitutive promoter marking cell outline with eGFP. HygR

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-35S:HygR p5-MpUBE2:eGFP-Lti6b
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV8-cPE-C

    Plasmid

    #183539

    Purpose

    Deliver a split compact Prime Editor in vivo

    Depositor

    Wen Xue

    Article

    Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

    Insert

    C-terminal fragment of SpCas9 nickase (H840A), Reverse Transcriptase
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    U1A

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-hSyn-evoCjCas9-ABE8e_gRNA

    Plasmid

    #202561

    Purpose

    hSyn-evoCjCas9-ABE8e and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-ABE8e
    

    Use

    AAV

    Available Since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Cas12j-8-puro

    Plasmid

    #194966

    Purpose

    The plasmid expresses human codon-optimized Cas12j-8 and puromycin resistence gene.

    Depositor

    Yongming Wang

    Article

    Wang et al Sci Adv. 2023 Feb 10;9(6):eabo6405. doi:

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-LAP2-mCherry

    Plasmid

    #159524

    Purpose

    Expresses mCherry from LAP2 promoter

    Depositor

    Esteban Engel

    Article

    Maturana et al Mol Ther Methods Clin Dev. 2020 Apr 14;1

    Insert

    mCherry
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    LAP2

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. FRB_LD 0_CS G_tTA-BFP

    Plasmid

    #58876

    Purpose

    MESA target chain with Rapamycin FRB ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA target chain with FRB ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    

    Use

    AAV

    Tags

    EBFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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