We narrowed to 9,109 results for: control

1. pmTurquoise2-T2A-Venus(L68V)

   Plasmid

   #60494

   Purpose

   Produces equimolar levels of mTurquoise2 and mVenus(L68V). It can be used as a negative control for FRET.

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   mTurquoise2-T2A-mVenus(L68V)
   

   Use

   Tags

   T2A peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOTTC407 - pAAV EF1a eGFP

   Plasmid

   #60058

   Purpose

   An AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

   Insert

   enhanced Green Fluorescent Protein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. L2092-GPA-FullBRET-Retrovirus-TD142

   Plasmid

   #222877

   Purpose

   Retroviral vector encoding positive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, RH linker, CyOFP1.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA-NanoLuc-CyOFP1
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV LTR

   Available since

   Aug. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV mGAD65(delE1) GFP WPRE SV40pA

   Plasmid

   #177316

   Purpose

   To produce the AAV vector that expresses GFP under the control of the mGAD65 promoter. The mGAD65 promoter has highly specificity to GABAergic interneurons.

   Depositor

   Hirokazu Hirai

   Article

   Hoshino et al Mol Brain. 2021 Feb 15;14(1):33. doi: 10

   Insert

   mGAD65(delE1) promoter, GABAergic interneurons specific promoter
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTet-O-RUNX3-T2A-PuroR

   Plasmid

   #162349

   Purpose

   Lentiviral expression of RUNX3 under the control of the TetON promoter

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   RUNX3 (RUNX3 Human)
   

   Use

   Lentiviral

   Tags

   T2A-PuroR

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-ON

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFB-U6-sgNeuN-gRNA-hSyn-mCherry-WPRE-SV40pA

   Plasmid

   #128346

   Purpose

   pAAV encoding control gRNA for CRISPR gRNAs listed above

   Depositor

   Ryan Drenan

   Article

   Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

   Insert

   Rbfox3 (NeuN) (Rbfox3 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. PL-SIN-EF1α-EGFP

   Plasmid

   #21320

   Purpose

   Control Lentiviral vector with strong EF1a promoter, expresses EGFP

   Depositor

   James Ellis

   Article

   Hotta et al Nat Methods. 2009 May . 6(5):370-6.

   Insert

   Enhanced Green Fluorescence Protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   June 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. BB3cH_pGAP_23*_pLAT1_Cas9

   Plasmid

   #104907

   Purpose

   hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Inserts

   HH - FS23 linker - HDV
   human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-hM3D(Gq)::RAM-d2tTA

   Plasmid

   #189627

   Purpose

   Expresses hM3D(Gq) under the hSyn promoter, and d2tTA under the pRAM (synthetic Fos) promoter. Plasmid 1 for recording Fos expression.

   Depositor

   Jerzy Szablowski

   Article

   Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Inserts

   hM3D(Gq)
   d2tTA
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn and pRAM

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-TetOne-Puro-LUC

    Plasmid

    #218630

    Purpose

    This plasmid contains luciferase under doxycycline inducible control.

    Depositor

    Valentin Cracan

    Article

    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

    Insert

    LUC (LOC116160065 )
    

    Use

    Lentiviral, Luciferase, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. L2125-FRB-GPA-NLuc-CyOFP1

    Plasmid

    #222885

    Purpose

    Positive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, 10 GS linker, CyOFP1.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA-20GS-NanoLuc-10GS-CyOFP1
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT3-EF1aH

    Plasmid

    #180149

    Purpose

    This plasmid is an empty vector used as the control for other plasmids which are in pT3-EF1aH backbone. It does not have any restriction site for cloning.

    Depositor

    Xin Chen

    Article

    Wang et al J Hepatol. 2021 Aug 28. pii: S0168-8278(

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRelA*

    Plasmid

    #175590

    Purpose

    Tet inducible, KAN Resistant, fluor labelled (YFP) synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.

    Depositor

    Greg Bokinsky

    Article

    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

    Insert

    Catalytic domain of E.coli relA gene.
    

    Use

    Tags

    Fused with a flexible GS linker to mVenus

    Expression

    Bacterial

    Mutation

    Only the catalytic domain of the enzyme is used (…

    Promoter

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV/L7-6-GFP-WPRE

    Plasmid

    #126462

    Purpose

    An AAV vector that expresses GFP under the control of the L7-6 promoter. The L7-6 promoter is the short size (0.8-kb) and has highly specificity to Purkinje cells.

    Depositor

    Hirokazu Hirai

    Article

    Nitta et al Mol Ther Methods Clin Dev. 2017 Jul 27;6

    Insert

    L7-6, Purkinje cell specific promoter
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-TORCAR(T/A)

    Plasmid

    #64928

    Purpose

    Negative-control mutant for TORCAR biosensor.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    TORCAR(T/A) (EIF4EBP1 Synthetic, Human)
    

    Use

    Tags

    Cerulean and YPet

    Expression

    Mammalian

    Mutation

    4EBP1 domain contains T37A and T46A mutations.

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS_3E5E_eGFP

    Plasmid

    #103054

    Purpose

    Encodes Ebola virus minigenome with EGFP reporter gene. Minigenome is under control of the CAG promoter

    Depositor

    Elke Mühlberger

    Article

    Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

    Insert

    Ebola virus (ebolavirus Zaire, Mayinga) minigenome
    

    Use

    Tags

    3E5E eGFP cloned into pCAGGS vector from pCDNA3.1…

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDA019

    Plasmid

    #131452

    Purpose

    control expression vector

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1a-DIO-EGFP-WPRE

    Plasmid

    #187103

    Purpose

    Double floxed EGFP under the control of EF1a promoter

    Depositor

    Yongling Zhu

    Article

    Xu et al Cell Rep. 2022 Jul 5;40(1):111036. doi:

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. p415 TEF roGFP2‐Tsa2ΔCR

    Plasmid

    #83238

    Purpose

    The genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.

    Depositor

    Tobias Dick

    Article

    Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

    Insert

    roGFP2-Tsa2dCr
    

    Use

    Tags

    roGFP2 fusion with Tsa2dCr

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMSCV-NLS-CBRed-d2-hygro

    Plasmid

    #139196

    Purpose

    non-ERK dependent control plasmid for pMSCV-NLS-CBGreen-FIRE-puro

    Depositor

    Matthew Lazzara

    Article

    Day et al Cell Rep. 2020 Mar 10;30(10):3383-3396.e

    Insert

    CBRed
    

    Use

    Luciferase and Retroviral

    Tags

    d2 PEST domain and nuclear localization signal (N…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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