2,226 results

1. pCMV5-FLAG-Gene33

   Plasmid

   #21633

   Depositor

   John Kyriakis

   Article

   Makkinje et al J Biol Chem. 2000 Jun 9. 275(23):17838-4

   Insert

   Gene33 (Errfi1 Rat)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 24, 2009

   Availability

   Academic Institutions and Nonprofits only

2. pBactin-Nedd1-mNeonGreen

   Plasmid

   #196864

   Purpose

   Expression of neural precursor cell expressed developmentally down-regulated 1 (Need1) fused to mNeonGreen

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Nedd1-mNeonGreen (Nedd1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBactin-td-mCherry-Cdk5rap2

   Plasmid

   #196859

   Purpose

   Expression of Cyclin dependent kinase 5 regulatory subunit-associated protein 2 (Cdk5rap2) fused to tandem (td)-mCherry.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   td-mCherry-Cdk5rap2 (Cdk5rap2 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CD200Cd4d3+4-blac

   Plasmid

   #32405

   Purpose

   A positive control receptor bait (rat CD4d3+4-blac) and prey (rat CD200) EXPRESs plasmid for The Basic AVEXIS kit

   Depositor

   Gavin Wright

   Article

   Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

   Insert

   CD200
   

   Use

   Tags

   Cd4d3+4 and beta-lactamase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-Syn-H2B-jGCaMP8s-WPRE

   Plasmid

   #176749

   Purpose

   AAV-mediated expression of histone fused GCaMP8m under the synapsin promoter

   Depositor

   Loren Looger

   Has Service

   AAV Retrograde, AAV1, and AAV9

   Insert

   H2B-jGCaMP8s
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   synapsin

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AAV-SYN_mMaroon-KIX-mMaroon

   Plasmid

   #137009

   Purpose

   AAV backbone, mMaroon-KIX acceptor for R-CREB

   Depositor

   Ryohei Yasuda

   Article

   Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

   Insert

   KIX domain (Crebbp Rat)
   

   Use

   AAV

   Tags

   mMaroon

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin

   Available since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. SaBE3-Gam

   Plasmid

   #100810

   Purpose

   Expresses SaBE3-Gam in mammalian cells

   Depositor

   David Liu

   Article

   Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

   Insert

   SaBE3-Gam
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Cherry-Myo1b

   Plasmid

   #187366

   Purpose

   Expresses rat myosin 1b isoform b domain labelled with Cherry

   Depositor

   Evelyne Coudrier

   Article

   Iuliano et al J Cell Biol. 2018 Jun 4;217(6):2033-2046

   Insert

   myosin 1b isoform b (Myo1b Rat)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HS-aPKC-CAAX-GFP

   Plasmid

   #105946

   Purpose

   heat shock inducible transgenesis

   Depositor

   Caren Norden

   Article

   Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

   Insert

   aPKC (Prkcz Rat)
   

   Use

   Tags

   CAAX and GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. XE241 Rat frizzled-1-GFP-CS2P+

    Plasmid

    #16817

    Depositor

    Randall Moon

    Article

    Kaykas et al Nat Cell Biol. 2004 Jan . 6(1):52-8.

    Insert

    frizzled-1 (Fzd1 Rat)
    

    Use

    Xenopus expression

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

11. FUap97GW (C4)

    Plasmid

    #74020

    Purpose

    lentiviral expression of SAP97alpha-EGFP fusion

    Depositor

    Weifeng Xu, Robert Malenka, Oliver Schluter

    Article

    Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

    Insert

    SAP97 alpha
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGP-CMV-jGCaMP8f F366H

    Plasmid

    #204141

    Purpose

    Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with F366H mutation

    Depositor

    Katalin Torok

    Article

    Tran et al Front Cell Neurosci. 2023 May 18;17:1155

    Insert

    jGCaMP8f F366H
    

    Use

    Tags

    N-terminal His tag

    Expression

    Mammalian

    Mutation

    Phenylalanine 366 changed to histidine

    Promoter

    CMV

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. iLID-tdTomato-Omp25

    Plasmid

    #214400

    Purpose

    Expresses fusion of iLID with tdTomato and transmembrane domain of Omp25

    Depositor

    Liting Duan

    Article

    Liu et al Proc Natl Acad Sci U S A. 2024 Apr 2;121

    Insert

    iLID-tdTomato-Omp25 (Synj2bp Rat, Synthetic)
    

    Use

    Tags

    iLID-TdTomato-Omp25

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. SyGCaMP5G

    Plasmid

    #162519

    Purpose

    Synaptically targeted GCaMP5G

    Depositor

    Josef Kittler

    Article

    Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

    Insert

    GCaMP5G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Erk2

    Plasmid

    #40777

    Purpose

    Expression of rat Erk2 in mammalian cells

    Depositor

    Dustin Maly

    Article

    Hari et al Chem Biol. 2014 May 22;21(5):628-35. doi

    Insert

    Erk2 (Mapk1 Rattus norvegicus)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Starting Met removed

    Promoter

    CMV

    Available since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

16. CMV-mEGFP(A206K)-paCaMKII(K42M)-ShadowG

    Plasmid

    #167477

    Purpose

    Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    mEGFP(A206K)-paCaMKII(K42M)-ShadowG
    

    Use

    Tags

    ShadowG and mEGFP(A206K)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CMV-mEGFP(A206K)-paCaMKII(T286A)-ShadowG

    Plasmid

    #167476

    Purpose

    Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    mEGFP(A206K)-paCaMKII(T286A)-ShadowG
    

    Use

    Tags

    ShadowG and mEGFP(A206K)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PKC zeta dNPS

    Plasmid

    #21250

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC zeta (Prkcz Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta 1-203

    Promoter

    Available since

    Nov. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

19. HA-RNF34

    Plasmid

    #119938

    Purpose

    RING finger protein 34 expression (HA-tag at the N-terminus)

    Depositor

    Angel de Blas

    Article

    Jin et al J Biol Chem. 2014 Oct 17;289(42):29420-3

    Insert

    RNF34 (Rnf34 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

20. Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A, E1707A) pCAGGS

    Plasmid

    #206115

    Purpose

    expression of rat Cav2.1 with an exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E320A, E670A, E1411A, E1707A

    Promoter

    CMV/B-actin

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Cav2.1 EA2, R57A, R59A pMT2

    Plasmid

    #206122

    Purpose

    expression of rabbit Cav2.1 calcium channel with a CaV2.1-P1217fs mutation causing a truncated protein, (EA2). R57A R59A mutations prevent dominant-negative suppression of Cav2.1 currents

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Neurobiol Dis. 2016 Sep;93:243-56. doi:

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cav2.1-P1217fs, R57A, R59A

    Promoter

    Ad MLP/TPL/SV40

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Cav2.1 EIVA (E1707A) pcDNA3

    Plasmid

    #206125

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain IV to to abolish divalent cation binding

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E1707A

    Promoter

    CMV

    Available since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Cav2.1 HA EIA (E320A) pcDNA3

    Plasmid

    #206124

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain I to abolish divalent cation binding

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E320A

    Promoter

    CMV

    Available since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A, E1707A) pcDNA3

    Plasmid

    #206121

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domains I, II, III and IV to to abolish divalent cation binding

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E320A, E670A, E1411A, E1707A

    Promoter

    CMV

    Available since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. KCC2-pHext

    Plasmid

    #104076

    Purpose

    To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)

    Depositor

    Igor Medyna

    Article

    Friedel et al eNeuro. 2017 Jul 21;4(4). pii: ENEURO.00

    Insert

    KCC2 (b isoform) (Slc12a5 Rat)
    

    Use

    Tags

    pHluorine tag inserted in the second putative tra…

    Expression

    Mammalian

    Mutation

    EcoR1 site in rat KCC2 was removed by silent muta…

    Promoter

    UbC

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. FUp95GW (B7)

    Plasmid

    #74016

    Purpose

    lentiviral expression of shRNA resistant Psd95-EGFP fusion

    Depositor

    Weifeng Xu, Robert Malenka, Oliver Schluter

    Article

    Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

    Insert

    Psd95 (DLG4 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pOC3 smFP-HA-Cacna1e KI

    Plasmid

    #183436

    Purpose

    CreON FlpOFF knock-in for smFP-HA-CaV2.3 (amino acid position: G5)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and smFP HA donor
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. FCIV1-GR

    Plasmid

    #72701

    Purpose

    Lentiviral expression of rat GR (2nd generation)

    Depositor

    Freddy Jeanneteau

    Article

    Lambert et al Mol Cell Biol. 2013 Sep;33(18):3700-14.

    Insert

    GR (Nr3c1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-Venus

    Expression

    Mammalian

    Mutation

    Promoter

    ubiquitin

    Available since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

29. F3/pRC-CMV

    Plasmid

    #197326

    Purpose

    Plasmid expressing an antigen targeting the Contactin/F3 scaffold protein

    Depositor

    Barbara Ranscht

    Insert

    Contactin/F3 (Cntn1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pZac2.1 gfaABC1D-BioID2-BioID2-Kir4.1-HA

    Plasmid

    #176746

    Purpose

    Expresses Kir4.1 fused with BioID2 at the N-terminus in astrocytes

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    HA-BioID2-BioID2-Kir4.1 (Kcnj10 Rat, Aquifex aeolicus)
    

    Use

    AAV

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    gfaABC1D

    Available since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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