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pCE046-SiT-Cas12a-[Repr]

Plasmid

#128132

Purpose

Express both AsCas12a fused to [Repr] repressor domain and CRISPR arrays on a single transcript

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-[Repr]-Triplex

Use

AAV

Expression

Mammalian

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-BRDT

Plasmid

#65381

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRDT (BRDT Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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pMSCV mSin1.1 HA

Plasmid

#12582

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Insert

mSin1.1 (MAPKAP1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Nov. 22, 2006

Availability

Academic Institutions and Nonprofits only
pBSSK WT p85 alpha flag

Plasmid

#13429

Depositor

Ronald Kahn

Article

Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

Insert

p85 alpha (PIK3R1 Human)

Tags

flag

Expression

Bacterial

Available Since

Nov. 1, 2006

Availability

Academic Institutions and Nonprofits only

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HIV-1 LTR-gfp

Plasmid

#115809

Purpose

HIV-1 LTR driven reporter vector that retains complete LTRs, tat, and rev, but has a frameshift mutation in env, an ngfr reporter gene in place of nef, and gfp in place of gag, pol, vif, and vpr

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

gfp and ngfr

Use

Lentiviral

Promoter

HIV1 LTR

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pR26 GFP Dest

Plasmid

#74283

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and GFP, for gateway cloning

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pAAVS1-P-CAG-GFPluc2

Plasmid

#80493

Purpose

donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP-luciferase fusion protein expression

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

GFPluc2

Use

Donor vector (human)

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-Utf1

Plasmid

#13369

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

undifferentiated embryonic cell transcription factor 1 (Utf1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
human MMP-2 promoter

Plasmid

#53432

Purpose

promoter activity study

Depositor

Etty Benveniste

Article

Qin et al J Biol Chem. 1999 Oct 8;274(41):29130-7.

Insert

MMP2 promoter (MMP2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

MMP2

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only

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pMK345 (Hygro-P2A-mAID-mClover)

Plasmid

#121179

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Hygro-P2A-mAID-mClover

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV5-CBFbeta

Plasmid

#12427

Depositor

Scott Hiebert

Article

Meyers et al Mol Cell Biol. 1995 Apr . 15(4):1974-82.

Insert

CBF beta (Cbfb Mouse)

Expression

Mammalian

Available Since

Nov. 17, 2006

Availability

Academic Institutions and Nonprofits only
plenti-ATF4

Plasmid

#125238

Purpose

ATF4 expression vector

Depositor

Rozita Yarmand

Article

Bagheri-Yarmand et al J Biol Chem. 2015 May 1;290(18):11749-61

Insert

ATF4 (ATF4 Human)

Use

Lentiviral

Tags

Flag

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcrDNA3.1-PRKACA

Plasmid

#100890

Purpose

Mammalian expression of PRKACA

Depositor

Sanford Simon

Article

Honeyman et al Science. 2014 Feb 28;343(6174):1010-4. d

Insert

Protein Kinase CAMP-Activated Catalytic Subunit Alpha (PRKACA Human)

Expression

Mammalian

Mutation

Isoform 1 wild type

Promoter

CMV

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pMT2 GATA1

Plasmid

#13626

Depositor

Anjana Rao

Article

Monticelli et al J Biol Chem. 2004 Aug 27. 279(35):36210-

Insert

GATA1 (Gata1 Mouse)

Expression

Mammalian

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pSLQ2818 pPB: CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

Plasmid

#84241

Purpose

Expresses ABA-inducible KRAB-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

ABI-tagBFP-Sp dCas9
PYL1-KRAB

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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Stat4 pRc/CMV

Plasmid

#8711

Depositor

Jim Darnell

Article

Zhong et al Proc Natl Acad Sci U S A 1994 May 24;91(

Insert

Stat4 (Stat4 Mouse)

Expression

Mammalian

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pJZC43

Plasmid

#66565

Purpose

sgRNA + 2XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 2XPP7
PCP-VP64 IRES mCherry

Expression

Mammalian

Promoter

CMV and U6

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pSFV3-lacZ

Plasmid

#92074

Purpose

This is an expression plasmid for production of recombinant genomic SFV RNA encoding beta-galactosidase.

Depositor

Henrik Garoff

Article

Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

Insert

lacZ

Use

Lacz expression plasmid, for run-off transcriptio…

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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M_ST1n_VPR

Plasmid

#63799

Purpose

ST1-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vector

Depositor

George Church

Article

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Insert

ST1-dCas9-VPR

Use

CRISPR and Synthetic Biology

Tags

VPR (VP64-p65-Rta)

Expression

Mammalian

Promoter

CMV

Available Since

April 14, 2015

Availability

Academic Institutions and Nonprofits only

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FR_HNF1B

Plasmid

#31101

Depositor

Gerhart Ryffel

Article

Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

Insert

HNF1B (HNF1B Human)

Use

Flp/frt

Tags

myc

Expression

Mammalian

Mutation

wild type

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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