We narrowed to 16,515 results for: puromycin

1. pLentiPGK Puro DEST JNKKTR EE Clover

   Plasmid

   #90239

   Purpose

   Lentiviral vector to express JNK KTR EE (mutant) mClover under PGK promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   JNK Kinase Translocation Reporter (EE mutant)
   

   Use

   Lentiviral

   Tags

   mClover

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX459-sgRNA2-RPB1-N-term

   Plasmid

   #195111

   Purpose

   Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).

   Depositor

   Job Dekker

   Article

   Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

   Insert

   sgRNA against first exon of RPB1 (N-terminal)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pICE-HA-CtIP-siR-WT

   Plasmid

   #82030

   Purpose

   Plasmid for constitutive or doxycycline-inducible expression of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Sébastien Britton, Patrick Calsou

   Article

   Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

   Insert

   CtIP (RBBP8 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Mutations making the cDNA resistant to a siRNA

   Promoter

   CMV-tet

   Available Since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-CMV-cGAS R71/75E-HA

   Plasmid

   #130919

   Purpose

   Expresses human cGAS-HA with R71/75E point mutation; Puromycin selection marker

   Depositor

   Jonathan Kagan

   Article

   Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

   Insert

   human cGAS R71/75E
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   aa 71 and 75 mutated to glutamate

   Promoter

   CMV

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti_Puro_DEST mouse_p65promoter_mouse_p65-Clover

   Plasmid

   #124549

   Purpose

   Lentiviral vector to express p65-Clover under p65 promoter (With puromycin resistance)

   Depositor

   Markus Covert

   Article

   Lane et al Cell Syst. 2017 Apr 26;4(4):458-469.e5.

   Insert

   p65 promoter- p65 CDS-Clover
   

   Use

   Lentiviral

   Tags

   Clover

   Expression

   Mammalian

   Available Since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDF1-ERBB4 (kinase dead)

   Plasmid

   #29544

   Depositor

   Yardena Samuels

   Article

   Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

   Insert

   ERBB4 (ERBB4 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   changed lysine 751 to methionine

   Available Since

   July 20, 2011

   Availability

   Academic Institutions and Nonprofits only

7. LentiGuide-Puro-CTRLg1 (CG509)

   Plasmid

   #139455

   Purpose

   Lentiviral vector with non-targeting gRNA and puromycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   Non-targeting sgRNA inserted; resistance gene: puroR
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a / U6

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. PL652

   Plasmid

   #159456

   Purpose

   Donor empty vector with puromycin resistance gene expression cassette flanked by FRT

   Depositor

   Yuejun Chen

   Article

   Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

   Type

   Empty backbone

   Use

   Cre/Lox

   Expression

   Mammalian

   Available Since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX459-sgRNA2-2-RPB1-C-term

   Plasmid

   #195109

   Purpose

   Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 C-term. Puromycin selection (2A-fusion).

   Depositor

   Job Dekker

   Article

   Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

   Insert

   sgRNA against last exon of RPB1 (C-terminal)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiguide-puro_hORAI1sg

    Plasmid

    #174326

    Purpose

    gRNA targeting hORAI1 - encodes puromycin resistance

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    human Orai1 (ORAI1 Human)
    

    Use

    Lentiviral

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYL236

    Plasmid

    #173184

    Purpose

    CRISPR donor plasmid for making the WT EZH2 control cell line. It contains a puromycin resistance gene and an mCherry gene

    Depositor

    Thomas Cech

    Article

    Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

    Insert

    EZH2 (EZH2 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX459-sgRNA4-CTCF-3p-UTR

    Plasmid

    #195106

    Purpose

    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting downstream of the human CTCF 3'UTR. Puromycin selection (2A-fusion).

    Depositor

    Job Dekker

    Article

    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

    Insert

    sgRNA against CTCF 3' UTR region
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX459-sgRNA3-CTCF-3p-UTR

    Plasmid

    #195105

    Purpose

    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting downstream of the human CTCF 3'UTR. Puromycin selection (2A-fusion).

    Depositor

    Job Dekker

    Article

    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

    Insert

    sgRNA against CTCF 3' UTR region
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiPGK Puro DEST JNKKTR AE Clover

    Plasmid

    #90240

    Purpose

    Lentiviral vector to express JNK KTR AE (mutant) mClover under PGK promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

    Insert

    JNK Kinase Translocation Reporter (AE mutant)
    

    Use

    Lentiviral

    Tags

    mClover

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX459-sgRNA3-RPB1-N-term

    Plasmid

    #195112

    Purpose

    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).

    Depositor

    Job Dekker

    Article

    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

    Insert

    sgRNA against first exon of RPB1 (N-terminal)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX459-sgRNA1-RPB1-N-term

    Plasmid

    #195110

    Purpose

    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).

    Depositor

    Job Dekker

    Article

    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

    Insert

    sgRNA against first exon of RPB1 (N-terminal)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR-U6gRNA-PGKpuro2ABFP

    Plasmid

    #136405

    Purpose

    Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses Puromycin linked to TagBFP.

    Depositor

    Massimo Squatrito

    Article

    Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Mutation

    BbsI restriction site at position 437 was removed…

    Promoter

    U6, PDK

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEF1α-M2rtTA-T2A-Puro

    Plasmid

    #169175

    Purpose

    piggyBac co-transfection transposon containing M2rtTA promoter linked with Puromycin resistance gene, through T2A with GSG linker, subcloned between ends of two ITRs. Pairs with #169174

    Depositor

    Igor Slukvin

    Article

    Jung et al Cell Rep. 2021 Feb 16;34(7):108758. doi:

    Insert

    M2rtTA-T2A-Puro
    

    Use

    Piggybac transposon

    Expression

    Mammalian

    Mutation

    See Depositor Comments Below

    Promoter

    EF1a

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. p221- purVSG117UTR

    Plasmid

    #59732

    Purpose

    Inserts a puromycin resistance gene and a VSG117 gene into the VSG221 expression site of T.brucei downstream of the expression site promoter.

    Depositor

    Gloria Rudenko

    Article

    Smith et al PLoS One. 2009 Oct 26;4(10):e7532. doi:

    Insert

    VSG117 flanked upstream and downstream by sequences homologous to the VSG221 expression site
    

    Use

    For homologous recombination into vsg221 expressi…

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-CMV-cGAS-N-HA (murine)

    Plasmid

    #130916

    Purpose

    Expresses murine cGAS N terminus(aa1-147)-HA; Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    murine cGAS N terminus
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    murine cGAS N terminus (aa 1-147)

    Promoter

    CMV

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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