We narrowed to 16,824 results for: puromycin
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Plasmid#59732PurposeInserts a puromycin resistance gene and a VSG117 gene into the VSG221 expression site of T.brucei downstream of the expression site promoter.DepositorInsertVSG117 flanked upstream and downstream by sequences homologous to the VSG221 expression site
UseFor homologous recombination into vsg221 expressi…Available SinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cas9-sgRNA-A+B
Plasmid#149370PurposeExpresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sitesDepositorInsertsCas9
sgRNA-A
sgRNA-B
Puromycin resistance
UseCRISPRTags3xFLAG, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianPromoterCBh, PGK, and U6Available SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX459-sgRNA4-CTCF-3p-UTR
Plasmid#195106PurposeVector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting downstream of the human CTCF 3'UTR. Puromycin selection (2A-fusion).DepositorInsertsgRNA against CTCF 3' UTR region
UseCRISPRExpressionMammalianAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX459-sgRNA3-CTCF-3p-UTR
Plasmid#195105PurposeVector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting downstream of the human CTCF 3'UTR. Puromycin selection (2A-fusion).DepositorInsertsgRNA against CTCF 3' UTR region
UseCRISPRExpressionMammalianAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8629 pHR-HREYBTATA-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-EFS-Puro-WPRE
Plasmid#140227PurposeHypoxia-inducible expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPR. Constitutive EFS-driven puromycin resistance.DepositorInsertzipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
UseLentiviralExpressionMammalianPromoterHRE (Hypoxia inducible)Available SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
HR180-LGR5-CreER
Plasmid#129093PurposeKI CreER with a puromycin/mRuby selection cassette into human LGR5 locusDepositorInsertCreERT2 and LGR5 homology arms (LGR5 Human)
UseHomologous recombination donor plasmidAvailable SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-M2rtTA-T2A-Puro
Plasmid#169175PurposepiggyBac co-transfection transposon containing M2rtTA promoter linked with Puromycin resistance gene, through T2A with GSG linker, subcloned between ends of two ITRs. Pairs with #169174DepositorInsertM2rtTA-T2A-Puro
UsePiggybac transposonExpressionMammalianMutationSee Depositor Comments BelowPromoterEF1aAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
piggyFlex
Plasmid#218234PurposeA piggyBac transposon-based gRNA expression vector, to allow for genomic integration and stable expression of gRNAs. Contains both antibiotic (puromycin) and fluorophore (GFP) markers.DepositorTypeEmpty backboneUseCRISPRAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAIP hIL4 co
Plasmid#74169PurposeLentiviral vector for expression of human codon optimized IL4 with puromycin resistance.DepositorAvailable SinceJune 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-cGAS-N-HA (murine)
Plasmid#130916PurposeExpresses murine cGAS N terminus(aa1-147)-HA; Puromycin selection markerDepositorInsertmurine cGAS N terminus
TagsHAExpressionMammalianMutationmurine cGAS N terminus (aa 1-147)PromoterCMVAvailable SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-RUNX1-WT
Plasmid#238536PurposeUsed as a positive control for overexpression of human RUNX1 in mammalian cells.DepositorAvailable SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pICE-FLAG-NAT10-siR-WT
Plasmid#59365PurposePlasmid for constitutive or doxycycline-inducible expression of wild-type human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInserthuman NAT10 (NAT10 Human)
TagsFLAGExpressionMammalianMutationMutations to render cDNA resistant to a siRNAPromoterCMV-tetAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Puro DEST PKAKTRClover
Plasmid#59153PurposeLentiviral vector to express PKA KTR mClover under PGK promoter (With Puromycin Resistance)DepositorAvailable SinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-cGAS C396/7A-HA
Plasmid#130917PurposeExpresses human cGAS-HA with C396/397A point mutations; Puromycin selection markerDepositorInsertcGAS C396/7A
TagsHAExpressionMammalianMutationmutated residues 396 and 397 to alaninesPromoterCMVAvailable SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-P-CAG-GFPluc2
Plasmid#80493Purposedonor vector for AAVS1 targeting (puromycin selection) and constitutive GFP-luciferase fusion protein expressionDepositorInsertGFPluc2
UseDonor vector (human)ExpressionMammalianPromoterCAGAvailable SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
LADL Anchor
Plasmid#127664PurposeEncodes the LADL Anchor (dCas9-CIBN fusion protein) and puromycin resistance both expressed from the EF1a promoterDepositorInsertdCas9-CIBN-2A-Puro
Tags3XFLAGExpressionMammalianMutationCas9 (D10A,H840A) mutantPromoterEF1aAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti_Puro_DEST mouse_p65promoter_mouse_p65-Clover
Plasmid#124549PurposeLentiviral vector to express p65-Clover under p65 promoter (With puromycin resistance)DepositorInsertp65 promoter- p65 CDS-Clover
UseLentiviralTagsCloverExpressionMammalianAvailable SinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKRG3-mU6-PUb-hSpCas9-pAc
Plasmid#162163PurposeExpresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectableDepositorInsertshSpCas9
sgRNA
UseCRISPRTagsT2A-pAcExpressionInsectPromoterAe. aegypti PUb and Ae. aegypti U6Available SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Transposon-for RMCE_Sleeping Beauty_LIR-CAG-Frt-red(katushka)-P2A-Puro-F3-PA-RIR
Plasmid#67275PurposeFor sleeping beauty transposition and establishment of an recombinase mediated cassette exchange docking site. To make RMCE in cell line instead of flp in cell linesDepositorInsertpuromycin resistant gene
Tagslinked to red flouresent protein gene (katushka)ExpressionMammalianPromoterCAGAvailable SinceJuly 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cag.Luc.puro
Plasmid#74409PurposeConstitutive Luciferase expression in mammalian cells along with puromycin selectionDepositorInsertLuciferase
ExpressionMammalianPromoterCAGAvailable SinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only