167,150 results
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Plasmid#140975PurposeEncodes a G alpha subunit (GNAI3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphai3-RLuc8 (GNAI3 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTKRED
Plasmid#41062DepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-CBE6a-V106W
Plasmid#215824PurposeExpress CBE6a-V106W (with SpCas9) in mammalian cellsDepositorInsertCBE6a V106W-SpCas9-2xUGI (cas9 )
UseIn vitro transcription templateAvailable SinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL056
Plasmid#177168PurposeExpresses wildtype MRE11 in human cellsDepositorAvailable SinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Puro-TK-NLS-mTurq2-PCNA
Plasmid#118617PurposeLentiviral expression of mTurq2 tagged PCNADepositorInsertTK-NLS-mTurq2-PCNA (PCNA Human)
UseLentiviralTagsmTurq2ExpressionMammalianPromoterhPGKAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJK148-Pnmt41-GBP-mCherry(N)-leu1+
Plasmid#89070Purposegenomic GBP-mCherry epitope N-terminal tagging at leu1+ locus in S. pombeDepositorTypeEmpty backboneUseGenomic integration in s. pombeTagsGBP-mCherryExpressionYeastAvailable SinceJune 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDB5321
Plasmid#204831PurposeAn integrating plasmid containing both the inducible enotetSW3 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetSW3 promoterAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-GG-hUbC-EBPF2-gRNA-SOX4
Plasmid#185552PurposeLentiviral backbone with EBFP2 reporter and gRNA targeting SOX4DepositorInsertSOX4 gRNAs
UseCRISPR and LentiviralExpressionMammalianAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
RGB-S reporter
Plasmid#207841PurposeA three-colour stress biosensor for real time analysis of physiological stress, genotoxicity, and cytotoxicity of Escherichia coliDepositorInsertsRpoH sensing construct
SOS sensing construct
RpoS sensing construct
ExpressionBacterialPromoterPosmY, PsulA, and grpEAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-tadDE-PcrA M6-UGI
Plasmid#221236PurposeExpress HACE Editor as a fusion of the engineered dual base editor TadE that can perform citosine and adenine deamination and PcrA helicase.DepositorInserttadDE-PcrA M6-UGI
ExpressionMammalianAvailable SinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRE-TIGHT-EGFP-backward donor
Plasmid#22077DepositorInsertTETO-eGFP
Available SinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
p4455 FLAG-hPLIC-2
Plasmid#8661DepositorAvailable SinceJune 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-beta2-adrenergic receptor
Plasmid#66994PurposeBeta-2-adrenergic receptor tagged with HaloTag on amino terminusDepositorInsertHaloTag-beta2-adrenergic receptor (ADRB2 Human)
TagsHuman Growth hormone Signal Sequence-HaloTagExpressionMammalianMutationThe signal sequence from human growth hormone was…PromoterCMVAvailable SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Cdc42-wt
Plasmid#12975DepositorAvailable SinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
CIBN-CAAX
Plasmid#79574Purposeexpression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targetingDepositorInsertCIBN (CIB1 Mustard Weed)
TagsC-terminal CAAX-boxExpressionMammalianMutationK93A, R94A, K106A, K107A (please see depositor co…PromoterCMVAvailable SinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEvolvR-enCas9-PolI5M
Plasmid#113078PurposeExpresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI5M
UseCRISPRExpressionBacterialAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiSSTe4_dTomato_nlsdTomato
Plasmid#213944PurposeAAV construct expressing dTomato driven by SST interneuron-targeting enhancer. Alias: AAV_WDS0004_dTomato_nlsdTomatoDepositorHas ServiceAAV PHP.eB and AAV1InsertdTomato_P2A_nls.dTomato
UseAAVAvailable SinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19_CRISPR_DpmrA
Plasmid#160903PurposeCRISPR_Cas9, lambda red recombineeringDepositorInsertsCas9
Lambda Red Recombineering Genes
Zeocin Resistance Cassette
PromoteraraBADAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Hb9-LMO3 (sbGLuc-VChR1-EYFP)
Plasmid#114103Purposefusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein (luminopsin LMO3) for bioluminescent optogeneticsDepositorInsertsbGLuc-VChR1-EYFP
UseAAVExpressionMammalianPromotermotoneuron-specific Homeobox 9 promoterAvailable SinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRPM4
Plasmid#25903DepositorAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
V96 ELP
Plasmid#68392PurposeElastin-like polypeptide (VPGxG) with 96 repeats of Valine guest residueDepositorInsertV96 ELP
ExpressionBacterialPromoterT7Available SinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFV2-HA
Plasmid#187082PurposeExpression of ATP synthase, with all Cys changed to Ala, and subunit a-HA tagged, subunit beta-His tagged)DepositorInsertatpBEFHAGDC
Tags6-His beta subunit (atpD) and HA tag subunit a (a…ExpressionBacterialMutationAll Cys codons changed to Ala.Available SinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_BMI1_WT
Plasmid#82194PurposeGateway Donor vector containing BMI1 , part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2-ccdB-Nanoluc
Plasmid#87075PurposeLentiviral Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferaseDepositorTypeEmpty backboneUseLentiviralTagsNanoluc luciferaseExpressionMammalianAvailable SinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLdCN2
Plasmid#125186PurposeExpresses Streptococcus pyogenes Cas9 (SpCas9) and two gRNAs in LeishmaniaDepositorInsertgRNA and Cas9
UseCRISPR; LeishmaniaAvailable SinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP
Plasmid#21181DepositorInsertenhanced green flourescent protein
UseDrosophila expressionAvailable SinceJune 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSB-CMV-MCS-Puro cPLA2-EGFP
Plasmid#162568PurposeUsed as a measure of cPLA2 activation in live cells with an EGFP tag.DepositorAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
plRL117
Plasmid#163635PurposeOptimized M. smegmatis CRISPRi plasmid; L5 integrating.DepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterTet-OnAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)
Plasmid#85008PurposeFor Flpe/FRT-based Supernova-GFP, pK068 should be used with pK036. The GFP fragment of pK068 can be replaced with another gene by SalI and EcoRV.DepositorInsertEGFP-ires-tTA
ExpressionMammalianAvailable SinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-Nucleus-7
Plasmid#55249PurposeLocalization: Nuclear Localization, Excitation: 383, Emission: 448DepositorInsertSV40 T-Antigen Nuclear Localization Signal (NLS)
TagsEBFP2-NLSExpressionMammalianPromoterCMVAvailable SinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits