We narrowed to 14,009 results for: flag
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Plasmid#231588PurposeALPK1 gene mutated in position V1092A (mutation found in spiradenomacarcinoma (sweat gland cancer)) under control of a doxycycline-inducible promoterDepositorInsertALPK1 (ALPK1 Human)
UseLentiviralTags3X flagExpressionMammalianMutationchanged Valine1092 to AlaninePromoterdoxycyclineAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 ALPK1 T237M
Plasmid#231586PurposeALPK1 gene mutated in position T237M (Pathogenic mutation in ROSAH syndrome) under control of a doxycycline-inducible promoterDepositorInsertALPK1 (ALPK1 Human)
UseLentiviralTags3X flagExpressionMammalianMutationchanged threonine 237 to MethioninePromoterdoxycyclineAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-hKCNQ3(R230C)-P2A-EGFP
Plasmid#209194PurposeCre-dependent expression of Kcnq3 with R230C mutation and GFP from the same transcriptDepositorInsertKcnq3 (KCNQ3 Human)
UseAAVTagsFLAGExpressionMammalianMutationR230 to C230PromoterCMVAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)
Plasmid#139997PurposeCMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterCMV and T7Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXS-mitoGshF-blasticidin
Plasmid#210314PurposeExpresses mitochondrially targeted, humanized Streptococcus thermophilus GshF in mammalian cellsDepositorInsertBifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
UseRetroviralTagsFLAG peptide, Homo sapiens ACO2 mitochondrial tar…ExpressionMutationCodon optimized for expression in human cellsPromoterAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-UbC-Tet1c-dCas9
Plasmid#232178PurposeLentiviral cassette that expresses Tet1c-dCas9 fusion with a short linker in mammalian cellsDepositorInsertTet1 catalytic domain - dead Cas9 - 2A - BlastR (TET1 Human, S. pyogenes)
UseLentiviralTagsFLAGExpressionMammalianMutationD10A, H840APromoterhUbCAvailable SinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLVgag-3xNES-ABE8e
Plasmid#181751PurposeMMLV-Gag fused to 3xNES-ABE8e with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.DepositorInsertMMLVgag-3xNES-ABE8e
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable SinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGHUC w-ERBIN (2-hybrid prey plasmid)
Plasmid#128163PurposeUV5 promoter drives expression of omega-erbin fusion. Pair with pB1H2 UV5 Zif268-cons as a positive control (turns on HIS3/GFP).DepositorInsertERBIN PDZ domain (ERBIN Synthetic)
UseSynthetic BiologyTagsFLAG and Omega subunit of RNA polymeraseExpressionBacterialMutationPromoterUV5Available SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLVgag-3xNES-ABE8e-NG
Plasmid#181754PurposeMMLV-Gag fused to 3xNES-ABE8e-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.DepositorInsertMMLVgag-3xNES-ABE8e-NG
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable SinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLVgag-3xNES-Cas9
Plasmid#181752PurposeMMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.DepositorInsertMMLV-Gag-3xNES-Cas9 (Spy)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable SinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40
Plasmid#101060PurposeAAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neuronsDepositorHas ServiceAAV1InsertCaMPARI2
UseAAVTagsFLAG-HA-myc and NES_hisExpressionMutationPromoterhsyn (synapsin-1)Available SinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGIPz-mPD-L1
Plasmid#121488PurposeExpression of mouse PD-L1 WTDepositorInsertPD-L1
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable SinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpRY-P2A-EGFP (RTW4830)
Plasmid#139989PurposeCMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterCMV and T7Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
CXCR4-Tango
Plasmid#66262PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-pA-M.EcoGII
Plasmid#192873PurposepA-M.EcoGII expression plasmid, originally created for BIND&MODIFY method, a Long-range single-molecule mapping of chromatin modification in eukaryotes. doi: https://doi.org/10.1101/2021.07.08.451578DepositorInsertpA-M.EcoGII
UseTags3X Flag TagExpressionBacterialMutationPromoterT7Available SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLVgag-3xNES-ABE7.10-NG
Plasmid#181753PurposeMMLV-Gag fused to 3xNES-ABE7.10-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.DepositorInsertMMLVgag-3xNES-ABE7.10-NG
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable SinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)
Plasmid#139996PurposeCMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…PromoterCMV and T7Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A-TAZ S89A
Plasmid#179911PurposeEntry vector of TAZ S89A for gateway coloning.DepositorInsertWW Domain Containing Transcription Regulator 1 (WWTR1 Human)
UseEntry vectorTags3X Flag-tagExpressionMutationSerine 89 mutated to AlaninePromoterAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
LPAR1-Tango
Plasmid#66418PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
dCas9-CBP FL
Plasmid#179550Purposeencodes S. pyogenes dCas9 with c-terminal fusion of full length human CBP driven by EF-1alpha promoterDepositorInsertS. pyogenes dCas9 with c-terminal fusion of full length human CBP (CREBBP Human)
UseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
EF.STAT3DN.Ubc.GFP
Plasmid#24984PurposeDepositorInsertSTAT3 (STAT3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationY705F--dominant negativePromoterAvailable SinceJune 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSIN4-EF1a-GATA1-IRES-Puro
Plasmid#61062Purposelentiviral vector for GATA1DepositorAvailable SinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSG5 EDD C2768A
Plasmid#37192DepositorInsertEDD C2768A (UBR5 Human)
UseTags6x His Tag and FLAGExpressionMammalianMutationChanged Cysteine 2768 to Alanine; K503R (please s…PromoterSV40Available SinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-EBF1
Plasmid#96965PurposeDox-inducible expression of Ebf1 in mammalian cellsDepositorAvailable SinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn1_nLightG
Plasmid#187179PurposeExpresses green norepinephrine indicator nLightG in neuronal cellsDepositorHas ServiceAAV9InsertnLightG
UseAAVTagsFlag tagExpressionMutationPromoterhuman Synapsin-1Available SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
LiOn-CMV∞Cre
Plasmid#154018PurposeVector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing a functional Cre recombinase from a CMV promoter upon action of the piggyBac transposaseDepositorInsertCre-FLAG
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK mKate2-OGT WT (Puro)
Plasmid#154290PurposeLentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, WT)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTags2x FLAG and mKate2ExpressionMammalianMutationWTPromoterPGKAvailable SinceDec. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FNLS-P2A-Puro
Plasmid#110841PurposeLentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)DepositorInsertBE3RA-FNLS
UseLentiviralTags3X FLAGExpressionMutationD10A and NLS sequence at the N-terminusPromoterEF1sAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hsyn_NES-his-CaMPARI2-F391W-G395D-WPRE-SV40
Plasmid#101063PurposeAAV vector expressing CaMPARI2_F391W-G395D (Kd = 530nM), a photoconvertible fluorescent protein-based calcium integrator, in neuronsDepositorInsertCaMPARI2_F391W-G395D
UseAAVTagsFLAG-HA-myc and NES_hisExpressionMutationPromoterhsyn (synapsin-1)Available SinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
TetO-dCas9-D3A
Plasmid#78254PurposeExpresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) linked to IRES-mCherry under the control of TetOp and a minimal CMV promoterDepositorInsertDNMT3A CD aa 598-912 (DNMT3A Human)
UseCRISPRTagsFlag epitope tagExpressionMammalianMutationPromoterCMVAvailable SinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only