We narrowed to 19,092 results for: iNOS

1. AAV phSyn1(S)-FlpE-bGHpA

   Plasmid

   #50364

   Purpose

   Can be used to generate AAV virus that will express FlpE recombinse gene in neurons from the synapsin promoter

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   FlpE recombinase gene
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn1

   Available Since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCEFL GAL4dbd-KLF4 1-156

   Plasmid

   #140147

   Purpose

   GAL4-DNA binding domain fusion protein with the 156 N-terminal amino acids of human KLF4 (activation domain)

   Depositor

   Ramiro Iglesias-Bartolome

   Article

   Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

   Insert

   KLF4 aa 1-156 (KLF4 Human)
   

   Use

   Tags

   GAL4dbd

   Expression

   Mammalian

   Mutation

   Deleted amino acids 157 to 479

   Promoter

   EF1a

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-nEF-Coff/Fon-bREACHes-EYFP

   Plasmid

   #137147

   Purpose

   Intersectional viral expression of bREACHes-EYFP in cells expressing Flp AND NOT Cre

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Insert

   Coff/Fon-bREACHes-EYFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   n/a

   Promoter

   nEF

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA8-Flag-LARS-DELTA-UNE-L

   Plasmid

   #139691

   Purpose

   Expresses N-teminal Flag tagged aa 1- 1064 of LARS1

   Depositor

   Jie Chen

   Article

   Yoon et al Cell Rep. 2016 Aug 9;16(6):1510-1517. do

   Insert

   LARS1 (LARS1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTE3328

   Plasmid

   #107539

   Purpose

   Expresses As crRNA and human codon optimized AsCpf1(RR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   As crRNA
   hAsCpf1(RR mutant)
   

   Use

   Tags

   3xHA, NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   S542R, K607R

   Promoter

   CMV and human U6

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF5/FRT/DEST-3xHA/mGli3/Flag P5-6A

   Plasmid

   #51249

   Purpose

   Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 980,1006 mutated to Ala

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli3 (Gli3 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV and Flag

   Expression

   Mammalian

   Mutation

   amino acids 980,1006 mutated to Ala

   Promoter

   EF1a

   Available Since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pTE3327

   Plasmid

   #107535

   Purpose

   Expresses As crRNA and human codon optimized AsCpf1(RVR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   As crRNA
   hAsCpf1(RVR mutant)
   

   Use

   Tags

   3xHA, NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   S542R, K548V, N552R

   Promoter

   CMV and human U6

   Available Since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-CCND1(T286A)

   Plasmid

   #172631

   Purpose

   Expresses untagged cyclin D1(T286A) in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Thr286Ala

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF5/FRT/DEST-3xHA/mGli3/Flag P1-4A

   Plasmid

   #51247

   Purpose

   Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907 mutated to Ala

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli3 (Gli3 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV and Flag

   Expression

   Mammalian

   Mutation

   amino acids 849,865,877,907 mutated to Ala

   Promoter

   EF1a

   Available Since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

10. W118-1_hRSK3(RPS6KA2)_K91A_K444A

    Plasmid

    #180385

    Purpose

    lentiviral transduction of human RSK3 (RPS6KA2) gene with point mutations (K91A and K444A) that inactivate kinase activity

    Depositor

    Huda Zoghbi

    Article

    Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

    Insert

    RPS6KA2_K91A/K444A (RPS6KA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    K91A/K444A

    Promoter

    CMV

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR-mCherry-p2A-LIC1 C-terminal half

    Plasmid

    #74603

    Purpose

    expresses LIC1 in mammalian cells; aa 389-523, untagged but cells indicate expression with diffuse mCherry

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    full length human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
    

    Use

    Lentiviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    amino acids 389-523 of LIC1

    Promoter

    SFFV

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. MEK1C218

    Plasmid

    #164639

    Purpose

    Bacterial expression plasmid for His6-MEK1C218

    Depositor

    Ben Davis

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
    

    Use

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    G(-19)F/S218C/C277S/C376S

    Promoter

    T7

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. RCAS-Neu HA SIINFEKL

    Plasmid

    #125832

    Purpose

    Retroviral expression of rat Neu cDNA

    Depositor

    Yi Li

    Article

    Du et al Proc Natl Acad Sci U S A. 2006 Nov 14;10

    Insert

    Neu (Erbb2 Rat)
    

    Use

    Retroviral

    Tags

    2x HA and SIINFEKL

    Expression

    Mammalian

    Mutation

    Val to Glu point mutation of codon 664 and trunca…

    Promoter

    Available Since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-iRFP-STOP-ReNL

    Plasmid

    #113965

    Purpose

    Piggybac transposon plasmid CRISPR gene deletion activatable fluorescence. Constitutive iRFP670 under EF1A promoter, CMV promoter with two SV40 polyA followed by red-enhanced nanolantern (ReNL)

    Depositor

    Jordan Green

    Article

    Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:

    Inserts

    iRFP670
    ReNL
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV - SV40-PolyA - SV40-PolyA and EF1A

    Available Since

    Dec. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-iLMO (slGLuc-Mac-EYFP)

    Plasmid

    #114100

    Purpose

    fusion protein of Gaussia luciferase variant superluminescent, Leptosphaeria maculans opsin, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    Gaussia luciferase, superluminescent; Leptosphaeria maculans opsin; EYFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2-mkgDUX4mal*leu*

    Plasmid

    #21173

    Depositor

    Stephen Tapscott

    Article

    Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

    Insert

    DUX4 (DUX4 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    First point mutation at nucleotide 5114 (numberin…

    Promoter

    Available Since

    Aug. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

17. XE130 beta2AR Fz2-Cs2+

    Plasmid

    #16794

    Depositor

    Randall Moon

    Article

    Liu et al Proc Natl Acad Sci U S A. 1999 Dec 7;96(

    Insert

    beta 2 AR Fz2 (Fzd1, Adrb2 Rat)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pEMS1162

    Plasmid

    #29077

    Purpose

    Expression of the eGFP reporter gene was not detected in the brain and eye.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple94 (GPR88 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-CARMA2sh-E142G

    Plasmid

    #97070

    Purpose

    Expresses Psoriasis-Linked mutant CARD14/CARMA2 in mammalian cells

    Depositor

    Pasquale Vito

    Article

    Scudiero et al Cell Death Dis. 2017 Feb 23;8(2):e2627.

    Insert

    CARD14 isoform 3 (CARD14 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Glutamic Acid 142 to Glycine

    Promoter

    CMV

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBrain-GFP-TACC3KDP∆682-688-shTACC3

    Plasmid

    #69116

    Purpose

    Dual-promoter plasmid to express knockdown-proof human TACC3 (682-688aa ch-TOG binding site deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.

    Depositor

    Stephen Royle

    Article

    Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

    Inserts

    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    shTACC3
    

    Use

    RNAi

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 682-688 (region containin…

    Promoter

    CMV

    Available Since

    Oct. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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