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Col1A-5'probe

Plasmid

#20732

Depositor

Rudolf Jaenisch

Article

Beard et al Genesis. 2006 Jan . 44(1):23-8.

Insert

Col1A-5'probe (Col1a1 Mouse)

Expression

Bacterial

Available Since

April 24, 2009

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-mouse Oct4

Plasmid

#21110

Depositor

Dean Tantin

Article

Kang et al Genes Dev. 2009 Jan 15. 23(2):208-22.

Insert

Oct4 (Pou5f1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
T7-RelA(K218R)

Plasmid

#23247

Depositor

Warner Greene

Article

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Insert

NFkB RelA(K218R) (RELA Human)

Tags

T7 gp10

Expression

Mammalian

Mutation

K218R

Available Since

March 15, 2010

Availability

Academic Institutions and Nonprofits only
pFP.R275

Plasmid

#138224

Purpose

Expresses the split mCherry reporter interrupted by the PhoRadA intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(SGK).PhoRadA.(TQL)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P(araBAD) and iPC

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P02

Plasmid

#120013

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with TagBFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

TagBFP

Use

Pcr template

Tags

TagBFP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z20

Plasmid

#120063

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 10xSuntag

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

10xSuntag

Use

Pcr template

Tags

10xSuntag

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV Puro-SV40:GFP

Plasmid

#68483

Purpose

Retrovirus expressing GFP driven by SV40 promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Puromycin Resistance
GFP

Use

Retroviral

Expression

Mammalian

Promoter

LTR and SV40

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pMP4516

Plasmid

#107775

Purpose

Expression of ECFP

Depositor

Herman Spaink

Article

Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

pLacZ-ECFP in pBBR1-mcs5

Tags

pLacZ-ECFP

Expression

Bacterial

Promoter

pLacZ

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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Flag TSC2 2A(S939A + S981A)

Plasmid

#12131

Depositor

Cheryl Walker

Article

Cai et al J Cell Biol. 2006 Apr 24. 173(2):279-89.

Insert

TSC2 S939A S981A (TSC2 Human)

Tags

Flag

Expression

Mammalian

Mutation

S939A. S981A.

Available Since

July 6, 2006

Availability

Academic Institutions and Nonprofits only
pMS32: pTwinStrep-SUMO-PmcTnsC

Plasmid

#168165

Purpose

PmcTnsC expression construct for protein purification.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

PmcCAST TnsC

Tags

6X His, Twin Strep and SUMO

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAW2-Smed-Slit-1

Plasmid

#182264

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Slit-1

Depositor

Daniel Lobo

Article

Wolff et al STAR Protoc. 2022 Jun 14;3(3):101458. do

Insert

Smed-Slit-1

Use

Gibson cloning vector for synthesis of single and…

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-mCherry2-G

Plasmid

#164162

Purpose

Expresses mCherry2 under the control of CAG promoter, basic vector, multiclonig site at C terminal

Depositor

Isei Tanida

Article

Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

Insert

mCherry2

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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gRNA-mCtnnb1

Plasmid

#84542

Purpose

mCtnnb1 gRNA expressing vector

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

Insert

mCtnnb1 gRNA

Use

CRISPR

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag DLC2

Plasmid

#24269

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

DLC2 (Dynll2 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
Munc18cwt-pDEST27

Plasmid

#85626

Purpose

GST tagged Munc18c

Depositor

David James

Article

Latham et al Traffic. 2006 Oct;7(10):1408-19. Epub 20

Insert

Munc18c (Stxbp3 Mouse)

Tags

GST

Expression

Mammalian

Promoter

CMV

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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Mouse NCKX3 (pcDNA3.1+)

Plasmid

#75204

Purpose

Mammalian expression of SLC24A3

Depositor

Jonathan Lytton

Article

Kraev et al J Biol Chem. 2001 Jun 22;276(25):23161-7

Insert

SLC24A3 (Slc24a3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pFbx15(-1172/+114)-Luc

Plasmid

#13412

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Upstream region of mouse Fbx15 gene

Use

Luciferase

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA5 FRT/TO MycLAP-STIL 5A

Plasmid

#80267

Purpose

mammalian expression plasmid for MycLAP-STIL 5A

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

STIL (STIL Human)

Tags

Myc-GFP

Expression

Mammalian

Mutation

S1103A, S1108A, S1111A, S1116A, T1119A

Promoter

CMV

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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TAL2385

Plasmid

#36794

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

BRCA1-TALEN_Right (Brca1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pDsRed (GB0100)

Plasmid

#68202

Purpose

Provides the CDS of DsRed as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

DsRed coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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