We narrowed to 11,806 results for: nsf

1. pGP-AAV-CAG-iGABASnFR2n-WPRE

   Plasmid

   #218884

   Purpose

   AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Insert

   iGABASnFR2n
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   S99A F104H R168P

   Promoter

   CAG

   Available Since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pssAAV_Seq17_CMV_bglobin_3NLS_Venus_WPRE_polyA

   Plasmid

   #220224

   Purpose

   AAV-STARR-seq validation control vector with Venus

   Depositor

   Simon Rumpel

   Article

   Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDGE667

   Plasmid

   #153232

   Purpose

   Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

   Depositor

   Sylvestre Marillonnet

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   GFP

   Expression

   Plant

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. GEARBOCS-SPARCL1-C-mCherryTag

   Plasmid

   #218183

   Purpose

   To tag Hevin with mCherry at its C-terminal

   Depositor

   Cagla Eroglu

   Article

   Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

   Insert

   sgRNA (Sparcl1 Mouse)
   

   Use

   AAV and CRISPR

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-TRE-mRuby3-WPRE

   Plasmid

   #193343

   Purpose

   AAV-mediated expression of mRuby3 under the TRE promoter in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

   Insert

   mRuby3
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA_Axin1 in pRK5

   Plasmid

   #154219

   Purpose

   N-terminal HA-tagged AXIN1 cDNA expression vector for transfections

   Depositor

   David Sabatini

   Article

   Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

   Insert

   AXIN1 (AXIN1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFX-GalT-mCherry

   Plasmid

   #174473

   Purpose

   Express mCherry with golgi apparatus-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   mCherry with golgi apparatus-targeting sequence
   

   Tags

   β1,4-galactose transferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpeCas9-HF

   Plasmid

   #192141

   Purpose

   Expresses highly specific SpeCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

   Insert

   SpeCas9-HF
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   SpeCas9-HF (R247A, N415A, S421A)

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CAG-CreiLOV-P2A-FusionRed

   Plasmid

   #192818

   Purpose

   Expresses CreiLOV with FusionRed tag in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

   Insert

   CreiLOV
   

   Use

   AAV

   Tags

   FusionRed

   Expression

   Mammalian

   Promoter

   chicken β-actin promoter

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CAG-SuperNovaGreen-T2A-dTomato

    Plasmid

    #193798

    Purpose

    Expresses SuperNova Green in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    SuperNova Green
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. px552-sg-gria1-HT-SEP

    Plasmid

    #187653

    Purpose

    Contains HaloTag-SEP to be inserted into the NTD of Gria1 (via HITI) and single guide RNA to target Cas9 to Gria1 under control of the U6 promoter.

    Depositor

    Erin O'Shea

    Article

    Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

    Insert

    HaloTag-SEP donor
    

    Use

    AAV and CRISPR

    Tags

    N/A

    Promoter

    N/A

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Str-KDEL_ST-SBP-tagBFP

    Plasmid

    #65266

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
    

    Tags

    Streptavidin Binding Protein (SBP) and tagBFP

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available Since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMCSG28-SH3-588

    Plasmid

    #185920

    Purpose

    Expresses SH3-588, a designed poly-proline peptide recruiting catechol O-methyltransferase

    Depositor

    Brian Kuhlman

    Article

    Park et al Nat Chem Biol. 2023 Apr;19(4):460-467. d

    Insert

    SH3-588
    

    Tags

    6x HIS tag

    Expression

    Bacterial

    Mutation

    Designed enzyme with novel interface between huma…

    Promoter

    T7

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTS105 pHAGE2 CMVtetO2 MCS-TEV-22xGS-GFP

    Plasmid

    #199358

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-GFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TEV-EGFP

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. (326) pAAV SV40-ZsGreen U6-gRNA

    Plasmid

    #163020

    Purpose

    Fluorescent reporter for Sa gRNA (Bsa1 sites)

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Zs Green
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. WDR24 in pRK5

    Plasmid

    #154213

    Purpose

    Untagged WDR24 cDNA expression vector for transfections

    Depositor

    David Sabatini

    Article

    Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

    Insert

    WDR24 (WDR24 Human)
    

    Tags

    NONE

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-DFO-ChR2(H134R)-EYFP-WPRE

    Plasmid

    #136916

    Purpose

    Expresses ChR2(H134R)-EYFP in Cre-negative cells

    Depositor

    Ofer Yizhar

    Article

    Sermet et al Elife. 2019 Dec 20;8. pii: 52665. doi: 1

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV

    Mutation

    H134R in ChR2

    Promoter

    Human Synapsin1

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDGE670

    Plasmid

    #153236

    Purpose

    Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

    Depositor

    Sylvestre Marillonnet

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-EF1a-Flex-CA-AMPK-H150R-T2A-mCherry

    Plasmid

    #83490

    Purpose

    cre-dependent CA-AMPK under EF1a promoter

    Depositor

    Dong Kong

    Article

    Kong et al Neuron. 2016 Jun 16. pii: S0896-6273(16)

    Insert

    CA-AMPK-2A-mCherry
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Mutation

    H150R

    Available Since

    Oct. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJC30X-BS(X)

    Plasmid

    #58958

    Purpose

    Encodes synthetic mitochondrial gene GFPm-3 in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.

    Depositor

    Thomas Fox

    Article

    Cohen et al Mitochondrion. 2001 Aug;1(2):181-9.

    Insert

    Enhanced fluorescence variant of GFP, in S. cerevisiae mitochondrial genetic code
    

    Tags

    First 8 codons of COX3

    Mutation

    GFP modified as described: CORMACK, B. P., R. H. …

    Promoter

    n/a

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only