161,273 results

1. pBW2661_pCAG-PV1-iCre-N270-L1-ABI-NLS-BGHpA

   Plasmid

   #108745

   Purpose

   Expresses N-terminus to aa270 of Cre recombinase fused to ABI CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Cre recombinase fragment N-terminus to aa270
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   ABI,NLS

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFUSEss-CHIg-mG3_M18

   Plasmid

   #82356

   Purpose

   Expression plasmid coding for heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18.

   Depositor

   Joanna Bereta

   Article

   Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103

   Insert

   mouse immunoglobulin heavy chain IgG3 isotype (Ighg3 Mouse)
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   none

   Promoter

   hEF1-HTLV prom

   Available since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMV:eGFP-p2A-HA-β2Ar-uTEV1Δ(220-242)

   Plasmid

   #135459

   Purpose

   Mammalian expression of SPARK protease component

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   eGFP-p2A-HA-βarrestin2-uTEV1Δ
   

   Use

   Lentiviral

   Tags

   eGFP, HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEN515 - pTRE3G-CTCF(ZFmut)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

   Plasmid

   #156434

   Purpose

   Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA (all Zinc fingers point mutated), targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA.

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   CTCF, rtta3G, TetO3G (Ctcf Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   All zinc fingers mutated

   Promoter

   Available since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpySwitch

   Plasmid

   #184225

   Purpose

   Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.

   Depositor

   Mark Howarth

   Article

   Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

   Insert

   SpySwitch
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   E77A mutation prevents isopeptide bond formation,…

   Promoter

   T7

   Available since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-Lyn-PlcR(K30/32L)

   Plasmid

   #181831

   Purpose

   Phospholipid binding-deficient Lyn-PlcR negative-control plasmid.

   Depositor

   Jin Zhang

   Article

   Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

   Insert

   Lyn-PlcR (K30L,K32L)
   

   Use

   Tags

   ECFP, EYFP, and N-terminal targeting sequence fro…

   Expression

   Mammalian

   Mutation

   Lysines 30 and 32 in PH domain mutated to leucine.

   Promoter

   CMV

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. ChlorON-3

   Plasmid

   #193479

   Purpose

   Turn-on fluorescent sensor for chloride

   Depositor

   Sheel Dodani

   Article

   Tutol et al ACS Cent Sci. 2023 Dec 18;10(1):77-86. d

   Insert

   ChlorON-3
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. alpha7nACHR_Nacho_mCherry

   Plasmid

   #182939

   Purpose

   alpha7 nACHR expression in mammalian cell lines

   Depositor

   Robert Brenner

   Article

   Belanger-Coast et al Eur J Pharmacol. 2022 Jun 15;925:175000.

   Insert

   NACH7 (CHRNA7 Human)
   

   Use

   Synthesized

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CAG-FLEX-EGFP

   Plasmid

   #59331

   Purpose

   Expresses EGFP

   Depositor

   Ian Wickersham

   Has Service

   AAV1

   Insert

   EGFP
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTETRIS-cargo

    Plasmid

    #126033

    Purpose

    Luciferase reporter construct for the study of cis-repressive activity in long noncoding RNAs. Also confers puromycin resistance.

    Depositor

    Mauro Calabrese

    Article

    Kirk et al Nat Genet. 2018 Oct;50(10):1474-1482. do

    Type

    Empty backbone

    Use

    Luciferase; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-T7-ABEmax(7.10)-SpCas9-NG-P2A-EGFP (RTW4564)

    Plasmid

    #140005

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized ABEmax(7.10) SpCas9-NG with P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-TIS11B CPM2

    Plasmid

    #121163

    Purpose

    Mammalian expression of GFP tagged TIS11B CPM2 (charge pattern mutant 2)

    Depositor

    Christine Mayr

    Article

    Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

    Insert

    ZFP36L1 (TIS11B) charge pattern mutant 2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. LeGO-EBFP2

    Plasmid

    #85213

    Purpose

    Lentiviral vector expressing EBFP2 fluorescent protein driven by SFFV promoter

    Depositor

    Boris Fehse

    Article

    Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

    Insert

    EBFP2
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-mGold2t-Tubulin-C-18

    Plasmid

    #231779

    Purpose

    Visualization of tubulin in mammalian cells using mGold2t

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    mGold2t-Tubulin-C-18 (TUBA1B Human)
    

    Use

    Tags

    mGold2s

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. U6>sgRNA(F+E)

    Plasmid

    #59986

    Purpose

    Empty vector for sgRNA cloning (F+E modified backbone)

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ciinte.U6

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRV.GFP FOXP3

    Plasmid

    #13250

    Depositor

    Anjana Rao

    Article

    Wu et al Cell. 2006 Jul 28. 126(2):375-87.

    Insert

    FOXP3 (FOXP3 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP-N1 hMOG-beta1-EGFP

    Plasmid

    #160978

    Purpose

    Expresses a human myelin oligodendrocyte glycoprotein isoform beta1 - EGFP fusion protein in mammalian cells

    Depositor

    Markus Reindl

    Article

    Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

    Insert

    Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant beta1 (MOG Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJAM1.20_PB-NEDD4L_deltaC2-PGK-Blast

    Plasmid

    #205565

    Purpose

    Express human NEDD4L (delta C2 isoform)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    NEDD4L (NEDD4L Human)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    delta C2 isoform

    Promoter

    EF1

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSL1180-HR-Aag2-loxP-PUb-RFP-loxP-3xFLAG-AGO1

    Plasmid

    #162164

    Purpose

    Selectable homology donor template for Aag2 cell AGO1

    Depositor

    Charles Rice

    Article

    Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

    Inserts

    RFP
    3xFLAG
    

    Use

    CRISPR and Cre/Lox; Homology donor template

    Tags

    AGO1 homology and loxP

    Expression

    Insect

    Mutation

    Promoter

    Ae. aegypti PUb and endogenous AGO1

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT3EF1aH-myr-Akt

    Plasmid

    #179909

    Purpose

    This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used with Cre for in vivo studies.

    Depositor

    Xin Chen

    Article

    Ho et al Hepatology. 2012 Mar;55(3):833-45. doi:

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Tags

    HA and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. BsaI-tagRFP-BsaI-pUC-kan

    Plasmid

    #170771

    Purpose

    test plasmid for Golden Gate assembly, encodes fluorescent red protein

    Depositor

    Ichiro Matsumura

    Article

    Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

    Insert

    tagRFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBW2663_pCAG-PV1-PYL-L1-iCre-271C-NLS-BGHpA

    Plasmid

    #108746

    Purpose

    Expresses aa271 to C-terminus of Cre recombinase fused to PYL CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Cre recombinase fragment aa271 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS and PYL

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. AiP13863 - pAAV-AiE0888m_C4-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3863)

    Plasmid

    #199773

    Purpose

    enhancer AAV for expression of SYFP2 in midbrain dopamine neurons

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Has Service

    AAV PHP.eB

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    wtGFP plus mutations F46L F64L S65G S72A M153T V1…

    Promoter

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGL4.23_Promoter_1-3

    Plasmid

    #210629

    Purpose

    Luciferase experiment for TAL1 promoter 1-3

    Depositor

    Maayan Salton

    Article

    Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

    Insert

    promoter 1-3 (TAL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal Promoter

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Flag-HA-USP15

    Plasmid

    #22570

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP15 (USP15 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pT3-Neo-EF1a-GFP

    Plasmid

    #69134

    Purpose

    Sleeping Beaquty (SB) vector encoding G418 resistance and GFP from two separate promoters

    Depositor

    Martin Bonamino

    Article

    Chicaybam et al Front Bioeng Biotechnol. 2017 Jan 23;4:9

    Type

    Empty backbone

    Use

    Sleeping beauty transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U3MSCV

    Available since

    Jan. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCS2-Gal4ff.zf1

    Plasmid

    #61392

    Purpose

    mRNA synthesis of Gal4ff codon modified for zebrafish

    Depositor

    Harold Burgess

    Article

    Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

    Insert

    Gal4ff.zf1
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    sp6

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSAP

    Plasmid

    #206429

    Purpose

    A vector backbone domesticated for SapI (pCC1BAC-based), contains elements for selection, maintenance, and propagation in S. cerevisiae (TRP1) and E. coli (cat).

    Depositor

    Bogumil Karas

    Article

    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHR_AXP_hrGFP

    Plasmid

    #84613

    Purpose

    HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into AXP locus

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al ACS Synth Biol. 2016 Dec 19.

    Insert

    hrGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    UAS1B8-TEF(136)

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Kras-Src FRET biosensor

    Plasmid

    #78302

    Purpose

    FRET biosensor. To monitor Src kinase activity in mammalian cells by FRET

    Depositor

    Yingxiao Wang

    Article

    Ouyang et al Proc Natl Acad Sci U S A. 2008 Sep 23;10

    Insert

    Src biosensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge