We narrowed to 21,140 results for: ACE;
-
Plasmid#207751PurposeDonor template for Blast-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748DepositorInsertACTB Homology Arms flanking a Blast-mNeon Cassette (ACTB Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a-Nlux
Plasmid#176239PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux under the control of Ribi promoterDepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferasePromoterRibosomal subunitAvailable SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(NT)-PGKpuro2ABFP-W
Plasmid#163169PurposeLentiviral plasmid with non-targeting gRNA, co-expression of BFP tagDepositorInsertNon-targeting
UseCRISPR and LentiviralTagsBFPExpressionMammalianPromoterU6Available SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCIG-FGFRdn
Plasmid#80431PurposeExpression of a dominant-negative FGFR1 with a nuclear EGFP reporter in chick embryosDepositorInsertFibroblast growth factor receptor 1 (FGFR1 Chicken)
TagsEGFPExpressionMammalianMutationcontains aa 1–425Promoterbeta-actin promoterAvailable SinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
GB1-A11(2-196, G175R)-Strep
Plasmid#196211PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G175R)-Strep.DepositorInsertAnnexin A11 (ANXA11 Human)
TagsGB1-6xHis-TEV and Strep-II tagExpressionBacterialMutationG175RAvailable SinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-A11(2-196, G189E)-Strep
Plasmid#196212PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G189E)-Strep.DepositorInsertAnnexin A11 (ANXA11 Human)
TagsGB1-6xHis-TEV and Strep-II tagExpressionBacterialMutationG189EAvailable SinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO-CreERT2 sgRipk4 v3
Plasmid#158048Purposelenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA against mouse Ripk4. NO Cas9DepositorInsertRipk4 sgRNA
ExpressionMammalianPromoterU6Available SinceOct. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
TetO-FUW-Hes1
Plasmid#61534PurposeExpresses Hes1 in mammalian cellsDepositorAvailable SinceJan. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCK069 desmaMCS:minprom_mApple,exorh:EGFP
Plasmid#195982PurposeExpression constructDepositorInsertdesmin a regulatory elements, mouse beta-globin minimal promoter mApple, exorh:EGFP (desma Zebrafish)
UseOtherAvailable SinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAB(EXPR-PYL-HDAC3-NEO)
Plasmid#114394PurposeFor PYL-HDAC3 expressionDepositorAvailable SinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO-CreERT2 sgAdam10 v3
Plasmid#158047Purposelenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA against mouse Adam10. NO Cas9DepositorInsertAdam10 sgRNA
ExpressionMammalianPromoterU6Available SinceOct. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCPP5912
Plasmid#128703PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcE-avrE1
ExpressionBacterialMutationThree synonymous mutations Ala 362 (GCT-GCC), Ala…Available SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-MEF2CA
Plasmid#61538PurposeExpresses MEF2CA in mammalian cellsDepositorAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
shLacZ
Plasmid#42559DepositorInsertshLacZ
UseLentiviral and RNAiExpressionMammalianAvailable SinceFeb. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pdCas12a(RuvC)
Plasmid#186447PurposeEncodes catalytically inactive Cas12a (D917A, E1006A) from F. novicida (type V-A)DepositorInsertdCas12a
UseCRISPRMutationD917A, E1006APromoterT7Available SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLEX_970_puro_DEST_V5_SKIL
Plasmid#45289DepositorAvailable SinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pB1H2 UV5 Zif268-5ala (2-hybrid bait plasmid)
Plasmid#128165PurposeUV5 promoter drives expression of Zif268-5alanine peptide fusion. Pair with pGHUC w-ERBIN as a negative control (doesn't turn on HIS3/GFP).DepositorInsert10 amino acid glycine/serine linker + 5alanines
UseSynthetic BiologyTagsFLAG and zif268ExpressionBacterialPromoterUV5Available SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-B57
Plasmid#135516PurposeMammalian expression of myc-tagged HLA-B*57:01DepositorInsertHLA-B*57:1 (HLA-B Human)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianPromoterCMVAvailable SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
TRMPVneo.Yap.3093
Plasmid#59915PurposeTet on shRNA for conditional Yap1 knockdownDepositorInsertYap1 (Yap1 Mouse)
UseRetroviralAvailable SinceSept. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-moxGFP-TUBA1B
Plasmid#207767PurposeDonor template for Puro-2A-moxGFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763DepositorInsertTUBA1B Homology Arms flanking a Puro-moxGFP Cassette (TUBA1B Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits