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pcDNA3-myc NES- mCE(K294A)

Plasmid

#82464

Purpose

Expresses myc tag and catalytic inactive form of mRNA capping enzyme with N terminal Nuclear export signal (NES)

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

NES mRNA capping enzyme (Rngtt Mouse, HIV)

Tags

myc

Expression

Mammalian

Mutation

K294A

Promoter

CMV

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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3186 pBS Bcl-XL Exon 1

Plasmid

#8755

Depositor

Stanley Korsmeyer

Article

Chao et al J Exp Med 1995 Sep 1;182(3):821-8.

Insert

Bcl-XL (Exon 1) (Bcl2l1 Mouse)

Expression

Bacterial

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
tdEos-SSTR3-N-17

Plasmid

#57670

Purpose

Localization: Cilium, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Insert

SSTR3

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pX330-sgRNA_Drosha_3

Plasmid

#79862

Purpose

CRISPR/Cas9 DROSHA

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

sgRNA Drosha

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
mEos2-CAF1-C-10

Plasmid

#57351

Purpose

Localization: Chromatin Assembly Factor 1, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

CAF1

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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M72-IRES-tauLacZ LTNL TV

Plasmid

#15638

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):817-31.

Insert

M72-IRES-tauLacZ LTNL TV

Use

High copy number

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-bio-myc-mCE 2-210

Plasmid

#112715

Purpose

Expresses N-terminally bio-myc-tagged mouse CE/Rngtt 2-210 in mammalian cells

Depositor

Daniel Schoenberg

Article

Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

Insert

Rngtt (Rngtt Mouse)

Tags

biotinylation signal peptide (Tagwerker et al. 20…

Expression

Mammalian

Mutation

Silent mutation at Arg 132 (CGT to CGC), deleted …

Promoter

CMV

Available Since

Oct. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pMIY-mETV3

Plasmid

#34578

Depositor

Peter Murray

Article

El Kasmi et al J Immunol. 2007 Dec 1;179(11):7215-9.

Insert

Etv3 (Etv3 Mouse)

Use

Retroviral

Tags

IRES-YFP

Expression

Mammalian

Available Since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only
nSR100L4

Plasmid

#35174

Depositor

Benjamin Blencowe

Article

Calarco et al Cell. 2009 Sep 4;138(5):898-910.

Insert

MmnSR100

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
mCitrine-VASP-5

Plasmid

#56571

Purpose

Localization: Actin/Focal Adhesions, Excitation: 516, Emission: 529

Depositor

Michael Davidson

Insert

VASP

Tags

mCitrine

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pMOR103-9-ISH

Plasmid

#15850

Depositor

Peter Mombaerts

Article

Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

Insert

MOR103-9 (Olfr1348 Mouse)

Use

Cloning vector

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
pEN_TTmiRC2_p53DD

Plasmid

#136520

Purpose

Used as a donor vector containing N-term 3xFLAG to clone into pSLIK

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

p53DD (Trp53 Mouse)

Use

Entry vector for gateway cloning

Tags

3x FLAG

Mutation

This construct was created by PCR from the pBabe …

Available Since

Oct. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Six4

Plasmid

#84323

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Six4. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Six4 (Six4 Mouse)

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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Antares-SEPLuc

Plasmid

#141086

Purpose

precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

Antares-T2A-puromycin-EMCV IRES-SEPLuc

Expression

Mammalian

Mutation

Deletion of amino acids 66 to 91 on the Luciferas…

Promoter

CMV

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-puro-mMcl-1 KR

Plasmid

#32981

Depositor

Joseph Opferman

Article

Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

Insert

Mcl-1 (Mcl1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

All Lys to Arg

Promoter

LTR

Available Since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3-HA/Bmal L115E

Plasmid

#47345

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Ha

Expression

Mammalian

Mutation

L115E

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
Nrp2.1-AP-His

Plasmid

#71972

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.1 (Nrp2 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pX330-sgRNA_Dicer_1

Plasmid

#68807

Purpose

specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.

Depositor

Constance Ciaudo

Article

Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

Insert

sgRNA mouse Dicer1

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221 - myc ULK1 4SA

Plasmid

#27625

Depositor

Reuben Shaw

Article

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

mouse myc ULK1 4SA (Myc Mouse)

Use

Gateway donr vector

Tags

myc

Mutation

S467A, S555A, T574A, S637A. Initial M from ULK1 r…

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pGL3-24p3/LCN2-145-LUC

Plasmid

#46866

Purpose

murine 24p3 minimal promoter as a luciferase reporter

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

24p3/lipocalin 2 proximal promoter

Use

Luciferase

Promoter

24p3 promoter (inactive)

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only