We narrowed to 156,005 results for: ins
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Plasmid#37686DepositorInsertRab2 (Rab2 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ65L (constitutive active)PromoterGAL4Available SinceNov. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab2 DN
Plasmid#37685DepositorInsertRab2 (Rab2 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationS20N (dominant negative)PromoterGAL4Available SinceNov. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
L13-Arl13bGFP
Plasmid#40879Purpose3rd generation transfer vectorDepositorAvailable SinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 F-tractin-EGFP
Plasmid#58473PurposeExpresses a cytoplasmic actin filament reporter, the neuronal inositol 1,4,5-triphosphate 3-kinase A actin-binding domain known as F-tractin, on a CMV promoterDepositorAvailable SinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pProEx-IDE-wt
Plasmid#99014PurposeExpress human Insulin Degrading Enzyme (IDE) in E. coli.DepositorAvailable SinceAug. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
3XFlagNICD4
Plasmid#20186DepositorInsertNotch 4 Intracellular Domain (Notch4 Mouse)
Tags3X FlagExpressionMammalianMutationContains murine Notch 4 Intracellular Domain (Val…PromoterCMVAvailable SinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_sfCherry3C(1-10)
Plasmid#117482PurposeLentiviral vector for constitutive expression of sfCherry3C(1-10)DepositorInsertsfCherry3C(1-10)
UseLentiviralExpressionMammalianMutation5 substitutions (K45R, G52D, T106A, K182R, N194D)…PromoterSFFVAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
3XFlagNICD1
Plasmid#20183DepositorInsertNotch 1 Intracellular Domain (Notch1 Mouse)
Tags3XFLAGExpressionMammalianMutationContains murine Notch 1 Intracellular Domain (Val…PromoterCMVAvailable SinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_sfCherry3V(1-10)
Plasmid#117483PurposeLentiviral vector for constitutive expression of sfCherry3V(1-10)DepositorInsertsfCherry3V(1-10)
UseLentiviralExpressionMammalianMutation6 substitutions (K45R, G52D, L83W, T106A, K182R, …PromoterSFFVAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
CIRTS-1: ORF5-TBP6.7-Pin domain-NLS
Plasmid#132543PurposeExpresses CIRTS-1 (ORF5-TBP6.7-Pin domain-NLS) in mammalian cellsDepositorInsertCIRTS-1: ORF5-TBP6.7-Pin domain-NLS
TagsFLAG tagExpressionMammalianPromotercmv d0Available SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
3XFlagNICD2
Plasmid#20184DepositorInsertNotch 2 intracellular domain (Notch2 Mouse)
Tags3X FLAGExpressionMammalianMutationContains murine Notch 2 Intracellular Domain (Met…PromoterCMVAvailable SinceJan. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10)
Plasmid#117484PurposeExpresses tandem binder (SpyCatcher002-6aa-sfCherry2(1-10)) in mammalian cellsDepositorInsertSpyCatcher002-6aa-sfCherry2(1-10)
ExpressionMammalianPromoterCMVAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
3XFlagNICD3
Plasmid#20185DepositorInsertNotch 3 Intracellular Domain (Notch3 Mouse)
Tags3X FlagExpressionMammalianMutationContains murine Notch 3 Intracellular Domain (Met…PromoterCMVAvailable SinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pShHELIX_sgRNA_entry (CJT32)
Plasmid#181782PurposeExpresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
ExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD287 (mKate2^SEC^3xMyc
Plasmid#70685PurposemKate2^SEC^3xMyc vector with ccdB sites for cloning homology armsDepositorInsertmKate2-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xMyc and C. elegans codon-optimized mKate2ExpressionWormAvailable SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mCherry-sfGFP-kanMX (pMaM17)
Plasmid#173452PurposeContains mCherry-sfGFP tandem fluorescent protein timerDepositorInsertmCherry-sfGFP-ADH1ter
ExpressionYeastPromoterno promoterAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
mPLD1-PX (71-210)
Plasmid#119127PurposeBacterial expression of human phox homology (PX) domain, mPLD1-PX (71-210)DepositorInsertmPLD1-PX (71-210)
TagsGSTExpressionBacterialPromotertacAvailable SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAcHELIX-sgRNA_entry (CJT94)
Plasmid#181783PurposeExpresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
ExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA4-NLS(SV40) (KAC200)
Plasmid#133801PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA4 with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA4
TagsNLS(SV40)ExpressionMammalianMutationn/aPromoterCMV and T7Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA5-NLS(SV40) (KAC203)
Plasmid#133802PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA5 with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA5
TagsNLS(SV40)ExpressionMammalianMutationn/aPromoterCMV and T7Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
p40phox-PX (1-144)
Plasmid#119123PurposeBacterial expression of human phox homology (PX) domain, p40phox-PX (1-144)DepositorInsertp40phox-PX (1-144)
TagsGSTExpressionBacterialPromotertacAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-Arl13b89
Plasmid#40874DepositorAvailable SinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pY2ShHELIX_sgRNA_entry (CJT30)
Plasmid#181781PurposeExpresses Y2-ShHELIX containing a nicking Y2 I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertY2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
ExpressionBacterialMutationY2-nAniI = F13Y, S111Y, K227M, F80K, L232KPromoterLac and J23119Available SinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
CIRTS-8: bdefensin 3-TBP6.7-hADAR(299-701)E488Q
Plasmid#132545PurposeExpresses CIRTS-8 (bdefensin 3-TBP6.7-hADAR(299-701)E488Q) in mammalian cellsDepositorInsertCIRTS-8: bdefensin 3-TBP6.7-hADAR(299-701)E488Q-NLS
TagsFLAG tagExpressionMammalianPromotercmv d0Available SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUAST td tomato Rab5 WT
Plasmid#58446Purposeexpression of drosophila td tomato Rab5DepositorAvailable SinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMAL_dstβL
Plasmid#73805PurposePlasmid for selection for insertion and homodimerizationDepositorInsertsCAT Marker
ToxR
beta lactamase
spectomycin resistance
CLS TM domain
TagsPart Of the dstbl chiemra, Part of the dsTbL chim…ExpressionBacterialPromoterNaN and ToxR promotorAvailable SinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-LMO11 (GeNL_SS-ChRmine)
Plasmid#213034Purposefusion protein of mNeonGreen, SSLuc, and ChRmine for bioluminescent optogeneticsDepositorInsertmNeonGreen, ISN-IE, ChRmine
UseAAVTagsGaussia luciferase signal sequencePromoterhSynAvailable SinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pShoHELIX-sgRNA_entry (BO3)
Plasmid#181784PurposeExpresses ShoHELIX containing a nicking I-AniI fusion to ShoTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
ExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR-Arl13b2
Plasmid#40871DepositorAvailable SinceNov. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUC-STITCH
Plasmid#129535PurposeSTITCH (Synthetic Toplogical Insulator with TetO for CHromatin-engineering) with homology arms for MYC locus insertion.DepositorInsertSTITCH
UseCRISPR; Donor templateAvailable SinceDec. 3, 2019AvailabilityAcademic Institutions and Nonprofits only