We narrowed to 5,620 results for: pcas

1. pCAG-MCS-EGFP

   Plasmid

   #164092

   Purpose

   expression of EGFP-tagged proteins under CAG promoter

   Depositor

   Maik Hintze

   Insert

   EGFP
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-mScarlet-Giantin

   Plasmid

   #196872

   Purpose

   Expresion of Giantin-fused to mScarlet. Used as Golgi marker

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mScarlet-Giantin (GOLGB1 Human, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Quimeric CAG

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pNS20-SpCas9-SNAP

   Plasmid

   #113717

   Purpose

   Bacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9

   Depositor

   Gerald Schwank

   Article

   Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   HA-2xNLS-SNAP-NLS and His6-MBP-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAGGS-6His-mISG15

   Plasmid

   #12445

   Depositor

   Dong-Er Zhang

   Article

   Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

   Insert

   ISG15
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pBW842_pCAG-pExpr-FP-DECODER

   Plasmid

   #87551

   Purpose

   Expresses BFP, GFP, iRFP, and mRuby in response to combinations of Cre and Flp recombinases

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   mtagBFP, EGFP, iRFP720, mRuby2
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG-SRGAP1-Myc

   Plasmid

   #168503

   Purpose

   Expression of full length SRGAP1 with a C-terminal Myc fusion

   Depositor

   Franck Polleux

   Article

   Coutinho-Budd et al J Cell Sci. 2012 Jul 15;125(Pt 14):3390-

   Insert

   SRGAP1 (SRGAP1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   aa 1-501

   Promoter

   CAG

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAT-YFP-TetR

   Plasmid

   #59018

   Purpose

   Expresses a fusion of yellow fluorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondii

   Depositor

   David Sibley

   Article

   Etheridge et al Cell Host Microbe. 2014 May 14;15(5):537

   Insert

   YFP-TetR
   

   Use

   Toxoplasma expression

   Tags

   Expression

   Mutation

   Promoter

   alpha-tubulin

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAX-mScarlet-FBP17

   Plasmid

   #178366

   Purpose

   Expresses FBP17 c-terminal labeled with mScarelt in mammalian cells

   Depositor

   Erik Dent

   Article

   Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

   Insert

   Formin Binding Protein 17 (FNBP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   400 Glutamic Acid changed to Aspartic acid (403 A…

   Promoter

   Chicken Beta Actin

   Available since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAS9-BFP empty

   Plasmid

   #80976

   Purpose

   Cas9, TagBFP, and sgRNA expression

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-Flp-DOG

    Plasmid

    #80084

    Purpose

    Flp recombinase dependent on GFP (Flp-DOG) for expression in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

    Insert

    Flp-DOG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG_Xph18-eGFP-CCR5TC

    Plasmid

    #135529

    Purpose

    Encodes a specific PSD-95 binder (Xph18) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph18
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAG-mCherry-ATG14

    Plasmid

    #203567

    Purpose

    Mammalian expression of mCherry tagged ATG14

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    ATG14 (ATG14 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBT224_(pCA-tTA2)

    Plasmid

    #36430

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Insert

    tetracycline transactivator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer)

    Available since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAIP-hXRCC1pd-eGFP

    Plasmid

    #206033

    Purpose

    Mammalian expression of PAR-deficient hXRCC1pd coupled to eGFP under the control of a CAG promoter

    Depositor

    Primo Schaer

    Article

    Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

    Insert

    hXRCC1
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S103A,R186A,S184A,S193A,S219A,S220A,S236A,S268A

    Promoter

    CAG

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-Gal4DBD-GBP2

    Plasmid

    #49439

    Purpose

    Expression of a GFP-dependent transcription factor component in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GBP2-Gal4 DNA binding domain fusion protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. G1397 DddAtox-N–dSpCas9

    Plasmid

    #157835

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    bpNLS–G1397 DddAtox-N–dSpCas9–UGI–UGI–bpNLS
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAX APP delta CT

    Plasmid

    #30143

    Depositor

    Dennis Selkoe, Tracy Young-Pearse

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein (APP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deletion of C-terminal intracellular domain (afte…

    Promoter

    Available since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

18. SpCas9 TadCBEa-V106W

    Plasmid

    #193838

    Purpose

    Express TadCBEa-V106W (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDa V106W-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX330-Flag-deSpCas9

    Plasmid

    #92114

    Purpose

    Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive eSpCas9 with FLAG tag
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A, K848A, K1003A, R1060A

    Promoter

    Cbh

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. eSpCas9(1.1)_No_FLAG

    Plasmid

    #79877

    Purpose

    FLAGless construct expressing high specificity eSpCas9(1.1). Px330-like plasmid.

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Untagged eSpCas9(1.1)

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCAGGS-3xFLAG-Rbfox2

    Plasmid

    #213726

    Purpose

    Mammalian cell expression vector for overexpression of Rbfox2 in motor neurons.

    Depositor

    Chaolin Zhang

    Article

    Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

    Insert

    Rbfox2 (Rbfox2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCAG-Bmpr2Δ751-813aa

    Plasmid

    #163603

    Purpose

    Expresses BMPR-2 lacking the LIMK-binding region in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The LIMK-binding region (751-813 aa) is deleted

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAG-GST-ATG14

    Plasmid

    #203566

    Purpose

    Mammalian expression of GST tagged ATG14

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    ATG14 (ATG14 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCAGGS-CD4-myc

    Plasmid

    #58537

    Purpose

    Expresses human CD4 with a C-terminal myc tag in mammalian cells

    Depositor

    Jacob Yount

    Article

    Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

    Insert

    CD4 (CD4 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pCAG vGlut1 GCaMP5G

    Plasmid

    #105008

    Purpose

    Presynaptic calcium sensor

    Depositor

    Franck Polleux

    Article

    Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

    Insert

    SLC17A7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCasper_Pdf-LexA::VP16

    Plasmid

    #33259

    Depositor

    Michael Rosbash

    Article

    Shang et al Proc Natl Acad Sci U S A. 2008 Dec 16;10

    Insert

    LexA::VP16-SV40 3'UTR (lexA E. coli)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Pdf

    Available since

    Dec. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

27. pCAG-Ace2_D92N_E199V-mNeon

    Plasmid

    #129256

    Purpose

    Express Ace2_D92N_E199V-mNeon in mammalian cells

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    Ace2_D92N_E199V-mNeon
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D92N, E199V

    Promoter

    CAG

    Available since

    Oct. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAG_Xph15-eGFP-CCR5TC

    Plasmid

    #135528

    Purpose

    Encodes a specific PSD-95 binder (Xph15) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph15
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLenti-nSpCas9-TadA8e

    Plasmid

    #209044

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8e (ABE8e) is fused to the N-terminus of SpCas9(D10A)-6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    TadA8e-nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAX APP C60

    Plasmid

    #30152

    Depositor

    Dennis Selkoe, Tracy Young-Pearse

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein C60
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    fragment

    Promoter

    Available since

    Aug. 15, 2011

    Availability

    Academic Institutions and Nonprofits only