We narrowed to 7,333 results for: ef1a
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Plasmid#194928Purposeexpressing two fusion proteins of Ag85B-ESAT6 and Rv2031c-Rv2626c from multi-stage antigens of Mycobacterium tuberculosisDepositorInsertsUseAdenoviralTagsAg85B-ESAT6, EF1a, and Rv2031c-Rv2626cMutationa 48 bp linker (Gly3ser)4 was added between the t…PromoterCMV and EF1aAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only
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Lenti_sgRNA(MS2)_zsGreen1
Plasmid#138463Purposelenti sgRNA cloning backbone with MS2 loops and EF1a-zsGreen1DepositorInsertzsGreen1
UseCRISPR and LentiviralExpressionMammalianPromoterEF1AAvailable SinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKAd5f11p-EPG
Plasmid#122697PurposeTo generate E1/E3-deleted human adenovirus 5 (HAdV-5) bearing a chimeric fiber and an EF1a promoter-controlled transgene.DepositorInsertGFP
UseAdenoviralPromoterEF1aAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Puro-24xMS2
Plasmid#212629PurposeMS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particlesDepositorInsertEF1a-PuroR-24xMS2-WPRE-hU6-sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterEF1aAvailable SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
JD141
Plasmid#229487PurposeEf1a promoter driving expression of adenoviral L4-22k/33k genesDepositorInsertL4-22k/33k
UseAAV and AdenoviralExpressionMammalianPromoterEf1aAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pONSY
Plasmid#111873Purpose"Capsaspora owczarzaki" expression vector (backbone) modified from the pCR2.1. It bears the promoter and terminator regions from the endogenous Elongation Factor 1 alpha (EF1a) gene (CAOG_07807).DepositorTypeEmpty backboneUseCapsaspora owczarzakiPromoterElongation Factor 1 alpha (EF1a) from CapsasporaAvailable SinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pZNpF-wt
Plasmid#245364PurposeDual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferaseDepositorInsertZfp423-wt (Zfp423 Mouse)
UseLuciferase and Synthetic BiologyTagsNanoLuciferaseExpressionMammalianPromoterEF1aAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB-HA-hGABARAP
Plasmid#229741PurposeExpresses HA-tagged human GABARAP in mammalian cells. Stable expression with piggybac transposase.DepositorAvailable SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLUEF1 IRES GFP (backbone)
Plasmid#224466PurposeEf1a downstream of a ubiquitous chromatin opening element drives expression of GOI and IRES GFPDepositorTypeEmpty backboneUseLentiviralPromoterEf1aAvailable SinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Puro-2xMS2
Plasmid#212627PurposeMS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particlesDepositorInsertEF1a-PuroR-2xMS2-WPRE-hU6-sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterEF1aAvailable SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Puro-12xMS2
Plasmid#212628PurposeMS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particlesDepositorInsertEF1a-PuroR-12xMS2-WPRE-hU6-sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterEF1aAvailable SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Puro-4xMS2
Plasmid#212626PurposeMS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particlesDepositorInsertEF1a-PuroR-4xMS2-WPRE-hU6-sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterEF1aAvailable SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEBB-SBP-HA
Plasmid#36100DepositorTypeEmpty backboneTagsHA and SBPExpressionMammalianPromoterEF1aAvailable SinceJune 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLV-mScarlet3-CAAX-IRES-Puro
Plasmid#222639PurposeLive-cell plasma membrane markerDepositorInsertmScarlet3-CAAX
UseLentiviralTagsmScarlet3PromoterEF1aAvailable SinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1
Plasmid#138460Purposelenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRABDepositorInsertMCP-KRAB-IRES-zsGreen1
UseCRISPR and LentiviralExpressionMammalianPromoterEF1AAvailable SinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti_sgRNA(MS2)_MCP-VP64-IRES-zsGreen1
Plasmid#138461Purposelenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-VP64DepositorInsertMCP-VP64-IRES-zsGreen1
UseCRISPR and LentiviralExpressionMammalianPromoterEF1AAvailable SinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT-EF-Pdgfralpha-EGFPN
Plasmid#66787PurposeExpression of murine PDGFRalpha tagged with GFP under the control of EF1a promoterDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
phMYT1L-N106
Plasmid#60861PurposeExpresses MYT1L under the EF1a promoterDepositorAvailable SinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-NBL10
Plasmid#69971PurposeCre-conditionally expresses the anti-GFP Nanobody-Rpl10a fusionDepositorInsertNBL10
UseAAV and Cre/LoxTagsHAExpressionMammalianPromoterEF1aAvailable SinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLUEF1 Luc2-IRES-GFP
Plasmid#224467PurposeEf1a downstream of a ubiquitous chromatin opening element drives expression of Luciferase2 and IRES GFPDepositorInsertLuciferase
UseLentiviralPromoterEF1aAvailable SinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-miRFP680-NES-p2A-dTomato-2xrGBD
Plasmid#222638PurposeRho reporterDepositorInsertmiRFP680-NES-p2A-dTomato-2xrGBD
UseLentiviralPromoterEF1aAvailable SinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-RhoB Sensor-IRES-Blast
Plasmid#222633PurposeRho reporterDepositorInsertRhoB Sensor
UseLentiviralPromoterEF1aAvailable SinceSept. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Empty Anchor Control
Plasmid#127665PurposeEncodes for puromycin resistance expressed from the EF1a promoterDepositorInsertPuro
ExpressionMammalianPromoterEF1aAvailable SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pONSY-mCherry
Plasmid#111874PurposeThis plasmid expresses mCherry fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.DepositorInsertmCherry
UseCapsaspora owczarzakiTagsmCherryPromoterElongation Factor 1 alpha (EF1a) from CapsasporaAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pONSY-Venus
Plasmid#111875PurposeThis plasmid expresses Venus fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.DepositorInsertVenus
UseCapsaspora owczarzakiTagsVenusPromoterElongation Factor 1 alpha (EF1a) from CapsasporaAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNpF-∆7
Plasmid#245367PurposeDual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferaseDepositorInsertZfp423-∆7 (Zfp423 Mouse)
UseLuciferase and Synthetic BiologyTagsNanoLuciferaseExpressionMammalianMutation7-bp deletion, H96Vfs*29PromoterEF1aAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZNpF-∆8
Plasmid#245368PurposeDual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferaseDepositorInsertZfp423-∆8 (Zfp423 Mouse)
UseLuciferase and Synthetic BiologyTagsNanoLuciferaseExpressionMammalianMutationsynthetic 8-bp deletionPromoterEF1aAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZNpF-∆59
Plasmid#245365PurposeDual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferaseDepositorInsertZfp423-∆59 (Zfp423 Mouse)
UseLuciferase and Synthetic BiologyTagsNanoLuciferaseExpressionMammalianMutation59-bp deletion, D78Vfs*6PromoterEF1aAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZNpF-∆11
Plasmid#245366PurposeDual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferaseDepositorInsertZfp423-∆11 (Zfp423 Mouse)
UseLuciferase and Synthetic BiologyTagsNanoLuciferaseExpressionMammalianMutation11-bp deletion, H96Wfs*4PromoterEF1aAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLX_TRC311
Plasmid#113668PurposeGateway destination vector for lentiviral expressionDepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterEF1aAvailable SinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-hTERT-IRES-hygro
Plasmid#85140PurposeLentiviral expression of hTERT, used to create immortalized cell linesDepositorAvailable SinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-EF1α-mKate2-biPAC
Plasmid#169127PurposeAAV plasmid containing a mKate2 and a light-gated adenylate cyclase biPACDepositorInsertmKate2-biPAC
UseAAV and Cre/LoxTagsmKate2ExpressionMammalianMutationcodon optimized to mousePromoterEF1aAvailable SinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
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HIVGKO
Plasmid#112234PurposeHIVGKO contains a codon-switched eGFP under the control of the HIV-1 specific promoter, and mKO2 under the control of the constitutive promoter EF1aDepositorInsertcsGFP-EF1a-mKO2
UseLentiviralAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EWB-DIO-cyan pre-eGRASP(p32)
Plasmid#111589PurposeAn AAV vector that expresses double floxed cyan pre-eGRASP(p32) under the Ef1a promoter.DepositorInsertcyan pre-eGRASP(p32)
UseAAVPromoterEf1aAvailable SinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDIO-DSE-mCherry-PSE-MCS
Plasmid#129669PurposeBackbone that can be used to generate AAV virus where, upon cre-specific recombination, mCherry and any shRNA can be simultaneously expressedDepositorTypeEmpty backboneUseAAVTagsmCherryPromoterEF1aAvailable SinceDec. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EWB-DIO-myriRFP670V5-P2A-post-eGRASP
Plasmid#111585PurposeAn AAV vector that expresses double floxed myristoylated iRFP670 with V5 tag and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.DepositorInsertmyriRFP670V5-P2A-post-eGRASP
UseAAVPromoterEf1aAvailable SinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-Ftractin-mRuby3-p2A-mTurquoise-MLC-IRES-Blast
Plasmid#85146Purposelentiviral expression of a stoichiometric F-actin and myosin light chain reporterDepositorInsertFtractin-mRuby3-P2A-mTurquoise-MLC
UseLentiviralTagsmRuby3, mTurquoisePromoterEF1aAvailable SinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EWB-DIO-cyan pre-eGRASP(p30)
Plasmid#111583PurposeAn AAV vector that expresses double floxed cyan pre-eGRASP(p30) under the Ef1a promoter.DepositorInsertcyan pre-eGRASP(p30)
UseAAVPromoterEf1aAvailable SinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT-EF-Pdgfralpha-EGFPN D842V
Plasmid#66789PurposeExpression of constitutively active murine PDGFRalpha D842V tagged with GFP under the control of EF1a promoterDepositorInsertPDGFRalpha (Pdgfra Mouse)
TagsEGFPExpressionMammalianMutationD842V (constitutively active)PromoterEF1aAvailable SinceJuly 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
Empty Target Control
Plasmid#127667PurposeEncodes for the CRY2-HA and mCherry proteins expressed from the EF1a promoterDepositorInsertCRY2-HA-2A-mCherry
TagsHAExpressionMammalianPromoterEF1aAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-RhoB Sensor (PKN-L59Q)-IRES-Blast
Plasmid#222634PurposeRho reporter, sensor deadDepositorInsertRhoB Sensor (PKN-L59Q)
UseLentiviralMutationPKN L59QPromoterEF1aAvailable SinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZNpF-∆11cag
Plasmid#245370PurposeDual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferaseDepositorInsertZfp423-∆11cag (Zfp423 Mouse)
UseLuciferase and Synthetic BiologyTagsNanoLuciferaseExpressionMammalianMutation11-bp deletion, H96Wfs*4, plus 3 synonymous mutat…PromoterEF1aAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZNpF-∆7cg
Plasmid#245371PurposeDual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferaseDepositorInsertZfp423-∆7cg (Zfp423 Mouse)
UseLuciferase and Synthetic BiologyTagsNanoLuciferaseExpressionMammalianMutation7-bp deletion, H96Vfs*29, plus 2 synonymous mutat…PromoterEF1aAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
phDLX1-N174
Plasmid#60859Purpose2nd generation lentiviral transfer plasmid. Expresses DLX1 under the EF1a promoterDepositorAvailable SinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
lenti dCAS-VP64_Blast
Plasmid#61425Purpose3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)DepositorHas ServiceLentiviral PrepInsertdCAS9(D10A, N863A)-VP64_2A_Blast
UseCRISPR and LentiviralExpressionMammalianMutationD10A and N863A in Cas9PromoterEF1AAvailable SinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
lenti sgRNA(MS2)_zeo backbone
Plasmid#61427Purpose3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6 and EF1AAvailable SinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
lenti sgRNA(MS2)_puro backbone
Plasmid#73795Purpose3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6 and EF1AAvailable SinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only