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We narrowed to 7,566 results for: ef1a

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Showing: 1901 - 1950 of 7566 results

  1. KZ801: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; EF1a promoter

    Plasmid
    #121814
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Gateway Cloning and Synthetic Biology
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121814-plasmid-map-sequence-id-237361

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  2. pCVL TLR 3.1 (Sce target) Ef1a iRFP Puro

    Plasmid
    #50417
    Purpose
    Fluorescent reporter for DNA cleavage/repair in mammalian cells expressing iRFP with selectable puromycin marker.
    Depositor
    Andrew Scharenberg
    Inserts
    eGFP with I-Sce I TS
    +3 mCherry
    Use
    Lentiviral
    Tags
    T2A dislinker and iRFP
    Expression
    Mammalian
    Mutation
    embedded I-Sce I TS from 163-185
    Promoter
    SFFV
    Available Since
    Jan. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    50417-plasmid-map-sequence-id-75349

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  3. KB601: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; EF1a promoter

    Plasmid
    #121819
    Purpose
    pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Gateway Cloning and Synthetic Biology
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121819-plasmid-map-sequence-id-237368

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  6. pAAV-EF1a-DIO-CoChR-Kv2.1-GP2A-NLS-eGFP

    Plasmid
    #253381
    Purpose
    Cre-dependent, soma-targeted CoChR with nuclear GFP expression
    Depositor
    Ofer Yizhar
    Insert
    st-CoChR-GP2A-NLS-eGFP
    Use
    AAV
    Expression
    Mammalian
    Available Since
    May 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    253381-plasmid-map-sequence-id-510497

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  7. (pRZ75) AAV: U6-gRNA(cs1)-EF1a-Cre-P2A-mCherry

    Plasmid
    #254581
    Purpose
    AAV backbone expressing Cre recombinase and mCherry from the EF1a promoter and one U6-driven sgRNA with SapI spacer and capture sequence in scaffold
    Depositor
    Jesse Engreitz
    Insert
    Cre
    Use
    AAV
    Available Since
    May 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    254581-plasmid-map-sequence-id-510214

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  19. pAK-DR30(SapI)-hfCas13d-Puro-pA/EF1A-mCherry-WPRE-pA

    Plasmid
    #233046
    Purpose
    To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site.
    Depositor
    Jonathan Ploski
    Article
    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3
    Insert
    RfxCas13d-compatible gRNA expression cassette and hfCas13d and mCherry
    Tags
    mCherry
    Expression
    Mammalian
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    233046-plasmid-map-sequence-id-467426

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  20. XZ363 EF1a-VEGFR1sp-3xFlag-VEGFR1(ecto+TM)-TS-ADAR2dd(QA)-export

    Plasmid
    #233382
    Purpose
    Optimized VEGF-sensing eLIDAR with VEGFR1 ecto+TM domains fused with ADAR2dd, with Golgi traffic sequence and ER export sequence
    Depositor
    Xiaojing Gao
    Article
    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038
    Insert
    VEGFR1(ecto+TM)-ADAR2dd
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    E488Q, T501A on ADAR2dd
    Promoter
    EF1a
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    233382-plasmid-map-sequence-id-466380

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  25. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide2

    Plasmid
    #118159
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA2 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a core and U6
    Available Since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118159-plasmid-map-sequence-id-240446

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  26. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide3

    Plasmid
    #118160
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA3 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a core and U6
    Available Since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118160-plasmid-map-sequence-id-240351

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  30. pAAV-Ef1a-Coff/Fon-taCasp3-T2A-TEVp-WPRE3-BGHpA

    Plasmid
    #242456
    Purpose
    AAV plasmid for expressing taCaspase-3 and T2A linked TEVp for caspase mediated cell ablation, and an intersectional strategy for Cre-off and Flp-on expression. See comments for more detail.
    Depositor
    Andrew Steele
    Insert
    Caspase 3 (CASP3 Synthetic, Human)
    Expression
    Mammalian
    Promoter
    Ef1a
    Available Since
    Dec. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    242456-plasmid-map-sequence-id-492090

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  33. Lenti-N1303K-tevopreQ1-epegRNA+13C>G_EF1a-puroR (PBS 14 - RTT 18)

    Plasmid
    #207356
    Purpose
    Lentiviral transfer plasmid encoding hU6-driven expression of a N1303K-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.
    Depositor
    Marianne Carlon
    Article
    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d
    Insert
    N1303K-CFTR + 13 C>G tevopreq1-prime editing guide RNA (epegRNA)
    Use
    Lentiviral
    Available Since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    207356-plasmid-map-sequence-id-408417

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  34. pGIPZ EF1a spIgE-3xFLAG-Neo2/15 IRES mNeonGreen-P2A-Puro

    Plasmid
    #248034
    Purpose
    Constitutively expresses 3xFLAG tagged Neoleukin 2/15 with an IgE signal peptide, mNeonGreen and a puromycin resistance gene in human cells
    Depositor
    Joshua Leonard
    Article
    Deol et al GEN Biotechnol. 2023 Jun 1;2(3):228-246.
    Insert
    3xFLAG-tagged Neoleukin-2/15
    Use
    Lentiviral
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    human EF1a
    Available Since
    Jan. 16, 2026
    Availability
    Academic Institutions and Nonprofits only
    248034-plasmid-map-sequence-id-497728

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  35. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA

    Plasmid
    #20297
    Purpose
    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation
    Depositor
    Karl Deisseroth
    Has Service
    AAV Retrograde, AAV1, AAV5, AAV8, and AAV9
    Insert
    channelrhodopsin-2
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    humanized ChR2 gene with histidine 134 changed to…
    Promoter
    EF1alpha
    Available Since
    June 23, 2009
    Availability
    Academic Institutions and Nonprofits only
    20297-plasmid-map-sequence-id-354478

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  38. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA

    Plasmid
    #20298
    Purpose
    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation
    Depositor
    Karl Deisseroth
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9
    Insert
    channelrhodopsin-2
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    humanized ChR2 gene with histidine 134 changed to…
    Promoter
    EF1alpha
    Available Since
    May 13, 2009
    Availability
    Academic Institutions and Nonprofits only
    20298-plasmid-map-sequence-id-191904

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  41. pJ204-loxP-EF1a-mCherry-NeoR-lox2272-BGHpA-siteA-HDR

    Plasmid
    #154826
    Purpose
    Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site A)
    Depositor
    Lise Marie Grav
    Article
    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102
    Inserts
    mCherry
    NeoR
    ZsGreen1-DR
    Use
    CRISPR and Cre/Lox
    Tags
    PEST
    Expression
    Mammalian
    Promoter
    CMV, EF1a, and SV40 early promoter
    Available Since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154826-plasmid-map-sequence-id-306204

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  43. pLVX-EF1alpha-SARS-CoV-2-nsp5-C145A-2xStrep-IRES-Puro

    Plasmid
    #141371
    Purpose
    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
    Depositor
    Nevan Krogan
    Article
    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:
    Insert
    SARS-CoV-2-nsp5-C145A (ORF1ab SARS-CoV-2)
    Use
    Lentiviral
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    C145A, human codon optimized
    Promoter
    EF1alpha
    Available Since
    April 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    141371-plasmid-map-sequence-id-288079

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  46. pAAV-EF1a>WAChR_1s-G-mScarlet-Soma-E; WPRE3-BGH polyA; AmpR

    Plasmid
    #247725
    Purpose
    Constitutive expression of WAChR-1s opsin fusion protein: {WAChR-1s}-{Kir2.1 Golgi tag}-{mScarlet}-{Kv2.1 soma tag}-{Kir2.1 ER tag}
    Depositor
    Alexander Naka
    Article
    Tose et al bioRxiv 2025.09.12.675947; doi: https://
    Insert
    WAChR_1s-G-mScarlet-Soma-E
    Use
    AAV
    Tags
    mScarlet
    Expression
    Mammalian
    Mutation
    F211W/F240A
    Promoter
    EF1a
    Available Since
    Dec. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    247725-plasmid-map-sequence-id-495874

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  47. pAAV-EF1a>WAChR_1f-G-mScarlet3-Soma-E; WPRE3-BGH polyA; AmpR

    Plasmid
    #247723
    Purpose
    Constitutive expression of WAChR-1f opsin fusion protein: {WAChR-1f}-{Kir2.1 Golgi tag}-{mScarlet3}-{Kv2.1 soma tag}-{Kir2.1 ER tag}
    Depositor
    Alexander Naka
    Article
    Tose et al bioRxiv 2025.09.12.675947; doi: https://
    Insert
    WAChR_1f-G-mScarlet3-Soma-E
    Use
    AAV
    Tags
    mScarlet3
    Expression
    Mammalian
    Mutation
    L130F/F240A
    Promoter
    EF1a
    Available Since
    Dec. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    247723-plasmid-map-sequence-id-495872

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  48. pAAV-EF1a>WAChR_1m-G-mScarlet-Soma-E; WPRE3-BGH polyA; AmpR

    Plasmid
    #247724
    Purpose
    Constitutive expression of WAChR-1m opsin fusion protein: {WAChR-1m}-{Kir2.1 Golgi tag}-{mScarlet}-{Kv2.1 soma tag}-{Kir2.1 ER tag}
    Depositor
    Alexander Naka
    Article
    Tose et al bioRxiv 2025.09.12.675947; doi: https://
    Insert
    WAChR_1m-G-mScarlet-Soma-E
    Use
    AAV
    Tags
    mScarlet
    Expression
    Mammalian
    Mutation
    S122L/F240A
    Promoter
    EF1a
    Available Since
    Dec. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    247724-plasmid-map-sequence-id-495858

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  50. PB-TRE-dCas9-VPR-pA-3xsgRNA-EF1a-Tet.O-T2A-PuroR-polyA

    Plasmid
    #166693
    Purpose
    Express dCas9-VPR in a doxycyclin-inducable system and 3 sgRNAs targeting the murine Cnga1 promoter. Can be stably integrated into the genome via the PiggyBac Transposon system.
    Depositor
    Elvir Becirovic
    Article
    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi
    Inserts
    dCas9-VPR
    3x Cnga1 promoter-targeting sgRNAs
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    TRE and U6
    Available Since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    166693-plasmid-map-sequence-id-331043

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Showing: 1901 - 1950 of 7566 results