We narrowed to 7,122 results for: ef1a

1. pLV Nup88-V5

   Plasmid

   #175117

   Purpose

   Lentiviral expression of mouse Nup88-V5

   Depositor

   Larry Gerace

   Insert

   Nup88 (Nup88 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLV Rbmxl1-V5

   Plasmid

   #175124

   Purpose

   Lentiviral expression of mouse Rbmxl1-V5

   Depositor

   Larry Gerace

   Insert

   Rbmxl1 (Rbmxl1 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. lenti-dCas9-VP64-BFP

   Plasmid

   #196711

   Purpose

   CRISPR-activation. dCas9-VP64 with TagBFP for sorting

   Depositor

   Bernhard Schmierer

   Insert

   dCas9-VP64-T2A-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   D10A, N863A

   Promoter

   EF1a

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. lenti-UCOE-dCas9-BFP-VP64

   Plasmid

   #196717

   Purpose

   CRISPR-activation. dCas9-VP64 with TagBFP for sorting.

   Depositor

   Bernhard Schmierer

   Insert

   dCas9-TagBFP-VP64
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   D10A, N863A

   Promoter

   EF1a

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLV-Puro_hCOL4A4-3FLAG

   Plasmid

   #198101

   Purpose

   lentiviral expression of hCOL4A4 in mammalian cells

   Depositor

   Jeffrey Miner

   Insert

   COL4A4 (COL4A4 Human)
   

   Use

   Lentiviral

   Tags

   3FLAG

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLV-Hygro_hCOL4A3

   Plasmid

   #198098

   Purpose

   lentiviral expression of hCOL4A3 in mammalian cells

   Depositor

   Jeffrey Miner

   Insert

   COL4A3 (COL4A3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ELL3 Streptag

   Plasmid

   #127282

   Purpose

   Expresses mouse ELL3 with streptactin tag; does it degrade Myc?

   Depositor

   Christine Milcarek

   Insert

   ELL3 (NM_145973) (ELL3 Mouse)
   

   Use

   Tags

   Twin strep-tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSBbi-Puro_LPAR_AKAR-WT

   Plasmid

   #125206

   Purpose

   SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based PKA activity reporter

   Depositor

   Eric Davis

   Insert

   LPAR_AKAR-WT
   

   Use

   Transposon

   Tags

   EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLV V5-Senp2

   Plasmid

   #175118

   Purpose

   Lentiviral expression of V5-tagged mouse Senp2

   Depositor

   Larry Gerace

   Insert

   Senp2 (Senp2 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. gGBEv6.3-SpCas9

    Plasmid

    #202629

    Purpose

    vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpCas9 nickase driven by EF1a promoter, guide RNA compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Natl Sci Rev. 2023 May 16;10(8):nwad143.

    Insert

    nSpCas9(D10A)-MPGv6.3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    MPGv6.3 = G163R, N169G, D175R, C178N, S198A, K202…

    Promoter

    hU6, EF1a, CBh

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLV-puro-N-3HA-SREBP1-C-Flag

    Plasmid

    #134286

    Purpose

    Lentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP1

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    SREBP1 (Srebf1 Mouse)
    

    Use

    Lentiviral

    Tags

    3x HA and Flag

    Expression

    Mammalian

    Mutation

    mouse SREBP1 isoform a precursor

    Promoter

    EF1a

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. gGBEv6.3-SpG

    Plasmid

    #202630

    Purpose

    vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpG nickase driven by EF1a promoter, guide RNA compatible with SpG driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Natl Sci Rev. 2023 May 16;10(8):nwad143.

    Insert

    nSpG(D10A)-MPGv6.3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SpG = D1135L, S1136W, G1218K, E1219Q, R1335Q, T13…

    Promoter

    hU6, EF1a, CBh

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. gCBEv2

    Plasmid

    #220617

    Purpose

    vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv2) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

    Insert

    hUNG2-nSpCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)

    Promoter

    hU6, EF1a

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. gTBEv3

    Plasmid

    #220619

    Purpose

    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv3) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

    Insert

    hUNG2-nSpCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

    Promoter

    hU6, EF1a

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-nEF Con/Foff hChR2(H134R)-EYFP

    Plasmid

    #55647

    Purpose

    Cre-on/Flp-off ChR2-EYFP under the short Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV; Cre on/flp off chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Short Ef1a

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LentiU6-LacZ-GFP-Puro (BB)

    Plasmid

    #170459

    Purpose

    For cloning gRNA block

    Depositor

    Yonglun Luo

    Article

    Xiang et al Nat Commun. 2021 May 28;12(1):3238. doi:

    Insert

    hU6-BB-LacZ and EF1A-EGFP-2A-Puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 for gRNA and EF1A for EGFP-2A-Puro

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-Tet-Off-FLEX-Lifeact-mScarlet-HA-WPRE-pA (JDW 721)

    Plasmid

    #229826

    Purpose

    Tet-off, AAV vector for cre dependent expression of Lifeact mScarlet tagged with HA with EFS (minimal EF1a promoter or EF1a short/ EFS) driving destablized tTA (d2tTA)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mScarlet-I
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS191

    Plasmid

    #197140

    Purpose

    scCloneSelect C-to-T reporter encoding BC-C1 barcode (EF1a promoter version)

    Depositor

    Nozomu Yachie

    Article

    Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    EGFP(M1V)

    Promoter

    EF1a

    Available since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV-Hyg-Snrnp40-skywarp-CRISPR-resistant

    Plasmid

    #134252

    Purpose

    Lentivector encoding CRISPR-resistant skywarp form of Snrnp40 (missing exon 5)

    Depositor

    Bruce Beutler

    Article

    Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

    Insert

    Snrp40 (Snrnp40 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    deleted amino acids 179-219, and mutated coding s…

    Promoter

    EF1a

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TK4-NLS-EGFP

    Plasmid

    #239046

    Purpose

    PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation

    Depositor

    Marius Wernig

    Inserts

    EGFP
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Tags

    T2A-mycNLS-mTagBFP2 and c-myc-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and EF1a

    Available since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. dCas9-miniTurbo

    Plasmid

    #219822

    Purpose

    EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)

    Depositor

    Philipp Maass

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    miniTurbo

    Expression

    Mammalian

    Mutation

    D10A/H841A

    Promoter

    EF1a

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPB-US-ECasE

    Plasmid

    #83961

    Purpose

    Piggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.

    Depositor

    Eleanor Chen

    Insert

    ERT2-spCas9-ERT2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    please see depositor comments below

    Promoter

    U6 (gRNA), EF1a (ERT2-Cas9-ERT2)

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLV-Blast_hCOL4A5

    Plasmid

    #198100

    Purpose

    lentiviral expression of hCOL4A5 in mammalian cells

    Depositor

    Jeffrey Miner

    Insert

    COL4A5 (COL4A5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CMV-AausFP1-Z1

    Plasmid

    #135048

    Purpose

    AausFP1 - bright green fluorescent protein expression from A. australis under CMV promoter in minimal backbone plasmid.

    Depositor

    Jordan Green

    Insert

    AausFP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLV-Blast_hCOL4A5-HA

    Plasmid

    #198103

    Purpose

    lentiviral expression of hCOL4A5 in mammalian cells

    Depositor

    Jeffrey Miner

    Insert

    COL4A5 (COL4A5 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Cofilin1_pLVX-EF1-Cry2-mCherry

    Plasmid

    #64370

    Purpose

    lentiviral expression of Cofilin1 and Cry2-mCherry

    Depositor

    Matthieu Piel

    Insert

    Cofilin1 (CFL1 Human)
    

    Use

    Lentiviral

    Tags

    Cry2-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pLV-Blast_hCOL4A5-HA_C1567R

    Plasmid

    #198105

    Purpose

    lentiviral expression of hCOL4A5_C1567R in mammalian cells

    Depositor

    Jeffrey Miner

    Insert

    COL4A5 (COL4A5 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    C1567R

    Promoter

    EF1a

    Available since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLV-Blast_hCOL4A5-HA_G869R

    Plasmid

    #198104

    Purpose

    lentiviral expression of hCOL4A5_G869R in mammalian cells

    Depositor

    Jeffrey Miner

    Insert

    COL4A5 (COL4A5 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    G869R

    Promoter

    EF1a

    Available since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSBbi-Pur-LPAR

    Plasmid

    #125205

    Purpose

    SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based kinase activity reporter with EV spacer, with AarI cassette for cloning of a kinase-target peptide

    Depositor

    Eric Davis

    Insert

    Lyn-Phospho-Activity-Reporter, Empty
    

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. lenti-dCas9-ZIM3-KRAB-BFP

    Plasmid

    #196712

    Purpose

    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3

    Depositor

    Bernhard Schmierer

    Insert

    dCas9-KRAB(ZIM3)-T2A-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pfwB

    Plasmid

    #37329

    Depositor

    John Schiller

    Article

    Buck et al J Virol. 2005 Mar;79(5):2839-46.

    Insert

    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pF2-DTA

    Plasmid

    #42521

    Depositor

    Terry Magnuson

    Article

    Jang et al PLoS One. 2013;8(2):e57075. doi: 10.1371

    Insert

    EF1a-DTA
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Aiolos in Artichoke

    Plasmid

    #74452

    Purpose

    Monitor post-translational degradation of Aiolos (Lentiviral, SFFV.Aiolos-17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

    Depositor

    Benjamin Ebert

    Insert

    Aiolos (IKZF3) (IKZF3 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    SFFV and EF1a

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. lenti-UCOE-dCas9-BFP-Zim3-KRAB

    Plasmid

    #196716

    Purpose

    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3

    Depositor

    Bernhard Schmierer

    Insert

    dCas9-TagBFP-KRAB(ZIM3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Limk1_pLVX-EF1-Cry2-mCherry

    Plasmid

    #64368

    Purpose

    lentiviral expression of Link1 and Cry2-mCherry

    Depositor

    Matthieu Piel

    Insert

    Limk1 (LIMK1 Human)
    

    Use

    Lentiviral

    Tags

    Cry2-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

36. miniTurbo-dCasRx

    Plasmid

    #219821

    Purpose

    EF1α-driven expression of miniTurbo fused to dCasRx with GFP (Adapted from plasmid #153209)

    Depositor

    Philipp Maass

    Insert

    dCasRx
    

    Use

    CRISPR

    Tags

    miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and hU6

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLV V5-lamin B2

    Plasmid

    #175109

    Purpose

    Lentiviral expression of V5-tagged mouse Lmnb2

    Depositor

    Larry Gerace

    Insert

    Lmnb2 (Lmnb2 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLV Erh-V5

    Plasmid

    #175123

    Purpose

    Lentiviral expression of mouse Erh-V5

    Depositor

    Larry Gerace

    Insert

    Erh (Erh Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLV_Hygro_mini-mAgrin-Myc-His

    Plasmid

    #198130

    Purpose

    Express mini-Agrin in mammalian cells

    Depositor

    Jeffrey Miner

    Insert

    mini-Agrin (Agrn Mouse)
    

    Use

    Lentiviral

    Tags

    TEV-Myc-His

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    June 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSBbi-Pur-Lyn-AktAR2-EV

    Plasmid

    #125199

    Purpose

    SB-transposon plasmid for stable expression of lipid raft-targeted Lyn-AktAR2-EV variant of FRET-based AKT activity reporter

    Depositor

    Eric Davis

    Insert

    Lyn-AktAR2-EV
    

    Use

    Transposon

    Tags

    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. ELL3 Streptag CtoA

    Plasmid

    #127283

    Purpose

    Expresses mouse ELL3 with streptactin tag; To interrogate if the mutation decreases putative Myc activity

    Depositor

    Christine Milcarek

    Insert

    ELL3 (NM_145973) (ELL3 Mouse)
    

    Use

    Tags

    Twin strep-tag

    Expression

    Mammalian

    Mutation

    CEYLHNK to AEYLHQK

    Promoter

    EF1a

    Available since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. ELL1 Streptag

    Plasmid

    #127278

    Purpose

    Expresses mouse ELL1 with streptactin tag in mammalian cells

    Depositor

    Christine Milcarek

    Insert

    ELL1 (NM_007924) (ELL Mouse)
    

    Use

    Tags

    Twin strep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. gTBEv5

    Plasmid

    #220621

    Purpose

    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv5) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

    Insert

    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

    Promoter

    hU6, EF1a

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. gCBEv3

    Plasmid

    #220618

    Purpose

    vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv3) driven by EF1a promoter, guide RNAs compatible with SpG driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

    Insert

    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)

    Promoter

    hU6, EF1a

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. gTBEv4

    Plasmid

    #220620

    Purpose

    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv4) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

    Insert

    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

    Promoter

    hU6, EF1a

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pT3-EFa1-HA-Jag1

    Plasmid

    #46051

    Depositor

    Xin Chen

    Article

    Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

    Insert

    Jag1 (Jag1 Rat)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pLV V5-lamin B1

    Plasmid

    #175108

    Purpose

    Lentiviral expression of V5-tagged mouse Lmnb1

    Depositor

    Larry Gerace

    Insert

    Lmnb1 (Lmnb1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pEF-GalNAcT2-moxGFP2

    Plasmid

    #218966

    Purpose

    Expresses full length GalNAcT2-moxGFP2 mammalian cells

    Depositor

    Benjamin Glick

    Insert

    GalNAcT2-moxGFP2 (GALNT2 Human)
    

    Use

    Tags

    moxGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pAAV hSyn-Flex RG-cerulean

    Plasmid

    #98221

    Purpose

    Cre dependent Cerulean-E2A-SADB19 rabies glycoprotein expression, under hSyn promoter, for producing AAV virus

    Depositor

    Troy Margrie

    Insert

    Cerulean-E2A-RG
    

    Use

    AAV

    Tags

    cerulean

    Expression

    Mutation

    Promoter

    human Synapsin

    Available since

    Aug. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. pLV Agpat2-V5

    Plasmid

    #175159

    Purpose

    Lentiviral expression of mouse Agpat2-V5

    Depositor

    Larry Gerace

    Insert

    Agpat2 (Agpat2 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge