We narrowed to 34,400 results for: CaS;

1. pCasKP-hph

   Plasmid

   #117232

   Purpose

   Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae

   Depositor

   Quanjiang Ji

   Article

   Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

   Insert

   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFGC-pcoCas9

   Plasmid

   #52256

   Purpose

   A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA

   Depositor

   Jen Sheen

   Insert

   35SPPDK:pcoCas9:NOS cassette
   

   Use

   CRISPR; Plant expression

   Tags

   Expression

   Mutation

   Promoter

   constitutive 35SPPDK promoter

   Available since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pdCas9-GFP

   Plasmid

   #181906

   Purpose

   Fluorescently tagged dead Cas9

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. SaCas9 TadCBEd

   Plasmid

   #193845

   Purpose

   Express TadCBEd (with SaCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadCBEd with SaCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Tet1-dCas9

   Plasmid

   #136650

   Purpose

   Tet1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s

   Insert

   Tet1 (Tet1 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLag-NLS-Tet1-dCas9-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cas9/pTREX-n

   Plasmid

   #68708

   Purpose

   Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.

   Depositor

   Roberto Docampo

   Article

   Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

   Insert

   Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
   

   Use

   CRISPR

   Tags

   Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …

   Expression

   Mutation

   C4239T mutation that eliminates a BamHI restricti…

   Promoter

   Available since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDN-Sth1Cas9

   Plasmid

   #221386

   Purpose

   Cas9 under control of AHT inducible TetR on integrating plasmid (KanR) using L5 integrase and attP for site-specific integration into attB

   Depositor

   Deborah Hung

   Article

   Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

   Insert

   Sth1 Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET47b-T7-enAsCas12f

   Plasmid

   #204642

   Purpose

   Expression of enAsCas12f in E. coli

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

   Insert

   enAsCas12f
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   D196K, N199K, G276R, N328G, D364R

   Promoter

   T7

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSET-dCas9

   Plasmid

   #110183

   Purpose

   Expression of the dCas9 gene in Streptomyces

   Depositor

   Yinhua Lu

   Article

   Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

   Insert

   dCas9
   

   Use

   CRISPR; I

   Tags

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   ermE*p

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cas9-GFP_sg_mTfeb

    Plasmid

    #79006

    Purpose

    Expresses WT Cas9 and GFP, along with a sgRNA to mouse Tfeb.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    sgTfeb (Tfeb Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. dSaCas9-VPR

    Plasmid

    #205251

    Purpose

    In vitro transcription plasmid for production of dSaCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #68495

    Depositor

    Rasmus Bak

    Article

    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

    Insert

    dSaCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9n(BB) (PX460)

    Plasmid

    #48873

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Has Service

    Cloning Grade DNA

    Insert

    hSpCas9n
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Cbh

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pColdI-DDX3_helicase_core

    Plasmid

    #233618

    Purpose

    Expression of His-tagged helicase core region of DDX3X in E.coli

    Depositor

    Shintaro Iwasaki

    Article

    Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

    Insert

    DDX3X helicase core WT (DDX3X Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. HiFi Cas9

    Plasmid

    #188490

    Purpose

    Expresses FLAG tagged HiFi Cas9

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    R691A

    Promoter

    EF1a

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. dCas9-CRY2Clust

    Plasmid

    #200969

    Purpose

    induction of phase-separated condensate by CRY2Clust

    Depositor

    Jian Xu

    Article

    Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

    Insert

    CRY2Clust
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAct-Cas9

    Plasmid

    #62209

    Purpose

    Expresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster.

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Insert

    cas9
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    act5C

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. SpCas9-NG-SsAPOBEC3B

    Plasmid

    #198536

    Purpose

    Expresses human codon-optimized SpCas9-NG-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NG-SsAPOBEC3B-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-SsAPOBEC3B
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSCAR_Cas9-hygro_GFP

    Plasmid

    #162075

    Purpose

    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and hygro resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Inserts

    Cas9-2a-hygromycin B phosphotransferase
    EGFP
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in M. musculus

    Promoter

    EF1a and sv40

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21b-Caspase-3

    Plasmid

    #90087

    Purpose

    Expresses wild type caspase 3 in bacterial cells

    Depositor

    Clay Clark

    Article

    Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

    Insert

    Caspase 3 (CASP3 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Wild type

    Promoter

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. SpRY Cas9

    Plasmid

    #188491

    Purpose

    Expresses FLAG tagged SpRY Cas9

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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