We narrowed to 55,043 results for: tra

1. pL6puro-JeT-pmRevErbA-Electra2-NLS-Pest

   Plasmid

   #240111

   Purpose

   A lentiviral (blue) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.

   Depositor

   Achim Kramer

   Article

   Gabriel et al bioRxiv 2025.06.30.661926

   Insert

   murine Nr1d1 promoter+Electra2-NLS-Pest-Reporter (Nr1d1 Mouse)
   

   Use

   Lentiviral

   Mutation

   added JeT Promoter

   Available Since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)

   Plasmid

   #47868

   Purpose

   This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.

   Depositor

   Erik Sontheimer, James Thomson

   Article

   Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

   Inserts

   NmCas9
   U6pr-tracrRNA
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   EF1a and U6

   Available Since

   Sept. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIG-641 JE-TRAC-BCMA(BB)28z-P2A

   Plasmid

   #186127

   Purpose

   BCMA-CAR DNA production

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   BCMA-CAR (TNFRSF17 Human)
   

   Use

   CRISPR

   Mutation

   WT

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. delta PE OCT4-GFP-2A-PURO transgenic reporter

   Plasmid

   #52380

   Purpose

   BAC recombineering generated construct, used as a reporter for OCT4 expression

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Inserts

   GFP-2A-puro
   OCT4 (POU5F1 Human)
   

   Expression

   Mammalian

   Mutation

   deleted -1620 - -1011 upstream from OCT4 ATG

   Available Since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. untargeted Aurora B FRET sensor Kif2 substrate

   Plasmid

   #45215

   Depositor

   Michael Lampson

   Article

   Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

   Insert

   untargeted Aurora B FRET sensor
   

   Tags

   CyPET, YPET

   Expression

   Mammalian

   Promoter

   t7

   Available Since

   May 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. Anti-Pan-Neurofascin (extracellular) [A12/18R]

   Plasmid

   #177438

   Purpose

   Mammalian Expression Plasmid of anti-Pan-Neurofascin (extracellular) (Rat). Derived from hybridoma A12/18.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-Pan-Neurofascin (extracellular) (Rattus norvegicus) recombinant mouse monoclonal antibody (Nfasc Mouse)
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. delta DE OCT4-GFP-2A-PURO transgenic reporter

   Plasmid

   #52381

   Purpose

   BAC recombineering generated construct, used as a reporter for OCT4 expression

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Inserts

   GFP-2A-puro
   OCT4 (POU5F1 Human)
   

   Expression

   Mammalian

   Mutation

   deleted -3194 - -1768 upstream from OCT4 ATG

   Promoter

   OCT4

   Available Since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. mouse deltaPE Oct4-GFP transgenic reporter

   Plasmid

   #52382

   Purpose

   BAC recombineering generated construct, used as a reporter for mouse Oct4 expression

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Inserts

   GFP pA
   Oct4 (Pou5f1 Mouse)
   PGK-gb2-Neo
   

   Expression

   Mammalian

   Mutation

   deleted -300 - -1300bp fragment upstream from Oct…

   Promoter

   Oct4 and PGK+gb2

   Available Since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEJS-HZ100 AAV-SpABE8e-C_terminus-tracrRNA

   Plasmid

   #211818

   Purpose

   AAV vector expressing C-terminus of SpCas9-ABE8e with tracrRNA

   Depositor

   Erik Sontheimer

   Article

   Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

   Insert

   C-terminus of SpCas9-ABE8e and tracrRNA
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   chicken β-actin promoter and U6 promoter

   Available Since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. mouse Oct4-GFP GOF18 transgenic reporter

    Plasmid

    #60527

    Purpose

    BAC recombineering generated construct, used as a reporter for Oct4 expression (containes both DE and PE elements)

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Insert

    GFPpA
    

    Expression

    Mammalian

    Promoter

    Oct4

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL4.23-IL2RA CaRE4 scramble (plus strand)

    Plasmid

    #91852

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0347)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE4 scramble (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Sequence scrambled from ch10:6094588-6094934

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLU-CMV-PL3 Flag-humanTRAF2-WT (1108)

    Plasmid

    #66924

    Purpose

    3rd generation lentiviral Expression of TRAF2

    Depositor

    Michael Karin

    Insert

    TRAF2 (TRAF2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXD017-39 TRAC PDCD1 AAV double KO AAV

    Plasmid

    #192153

    Purpose

    TRAC PDCD1 AAV double KO AAV

    Depositor

    Sidi Chen

    Article

    Dai et al Nat Methods. 2019 Mar;16(3):247-254. doi

    Insert

    sgRNA
    

    Use

    AAV

    Mutation

    NA

    Available Since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. untargeted Plk1 FRET sensor Myt1 substrate

    Plasmid

    #45217

    Depositor

    Michael Lampson

    Article

    Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

    Insert

    untargeted Plk1 FRET sensor Myt1 substrate
    

    Expression

    Mammalian

    Promoter

    t7

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAd-Track Flag GCN5 Y621A/P622A

    Plasmid

    #14425

    Depositor

    Pere Puigserver

    Article

    Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

    Insert

    GCN5 Y621A/P622A (KAT2A Human)
    

    Use

    Adenoviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Y621A, P622A. Catalytically inactive acetyltransf…

    Available Since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1-Tras heavy chain-GSG-SpyTag003

    Plasmid

    #216292

    Purpose

    Expresses anti-HER2 Tras Heavy chain-GSG-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    Tras heavy chain-GSG-SpyTag003
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6 tag, Signal peptide, and SpyTag003

    Expression

    Mammalian

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSIN-U6-tracer_C9_1_-EF1a-Thy1.1-P2A-Neo

    Plasmid

    #191397

    Purpose

    sgRNA targeting alpha-satellites on human chromosome 9

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    sgRNA targeting alpha satellites on human chromosome 9
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTransgenesis p3 sv40 minimal promoter:: Katushka RFP

    Plasmid

    #131731

    Purpose

    Transposon based transgenesis

    Depositor

    Rene Maehr

    Article

    Stanney W et al Dev Biol. 2020 Mar 15;459(2):161-180. do

    Insert

    SV40MinPromoter
    

    Use

    Xenopus expression

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLU-CMV-PL3 Flag-humanTRAF3-WT (1114)

    Plasmid

    #66922

    Purpose

    Lentiviral Expression of TRAF3

    Depositor

    Michael Karin

    Insert

    TRAF3 (TRAF3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract

    Plasmid

    #138525

    Purpose

    Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.

    Depositor

    Richard Jenner

    Article

    Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

    Insert

    Control non-PRC2 binding A-tract repeat RNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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