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pCbs FlagOmomyc

Plasmid

#113168

Purpose

Expression of FLAG tagged Omomyc in mammalian cells

Depositor

Sergio Nasi

Publication

Savino et al PLoS One. 2011;6(7):e22284. doi: 10.1371

Insert

Omomyc (MYC Human)

Tags

FLAG

Expression

Mammalian

Mutation

E57T, E64I, R70Q, R71N

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET24a-VHH-std

Plasmid

#109417

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody. VHH-std also contains a T7, HA, BAP and His6 epitope

Depositor

Martin Spiess

Publication

Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

anti-GFP nanobody fused to a T7, HA, BAP and His6 epitope

Tags

BAP, HA, His6, and T7

Expression

Bacterial

Promoter

T7

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDisplay-R-iGluSnFR1

Plasmid

#107335

Purpose

Red fluorescent glutamate sensor based on cpmApple. Membrane-displayed

Depositor

Robert Campbell

Publication

Wu et al ACS Chem Biol. 2018 Jan 22. doi: 10.1021

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

R-iGluSnFR1

Expression

Mammalian

Promoter

CMV

Available since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pKA-142

Plasmid

#79835

Purpose

Expresses PpsR2-mVenus-CAAX under CMVd1 promoter

Depositor

Vladislav Verkhusha

Publication

Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PpsR2-mVenus-CAAX

Expression

Mammalian

Mutation

RpPpsR2 cysteine 439 changed to serine

Promoter

CMVd1

Available since

Aug. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Sumo3 PolQM1

Plasmid

#78462

Purpose

Inducible expression in E. coli

Depositor

Sylvie Doublie, Susan Wallace

Publication

Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

Insert

Polymerase theta (POLQ Human)

Tags

His and SUMO3

Expression

Bacterial

Mutation

delta 1-1791; Q2513R (please see depositor commen…

Promoter

T7

Available since

July 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
M78 pIRESpuro-CBP-2xTEV-StrepTag

Plasmid

#17132

Depositor

Randall Moon

Publication

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Type

Empty backbone

Tags

2xTEV, CBP, and Streptavidin

Expression

Mammalian

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pOpen-phi29pol

Plasmid

#165573

Purpose

DNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

phi29 DNA Polymerase

Use

Synthetic Biology

Available since

Aug. 24, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CXCR3-Tango

Plasmid

#66261

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Publication

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CXCR3 (CXCR3 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pST39

Plasmid

#64009

Purpose

original polycistronic cloning vector for plasmid suite (generation I)

Depositor

Song Tan

Publication

Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pRPN265NB-LaG16-MNase

Plasmid

#170978

Purpose

Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

Depositor

Marc Timmers

Publication

Koidl et al Curr Protoc. 2021 Oct;1(10):e266. doi: 1

Insert

Glutathione-S-Transferase/LaG16/MNase

Tags

GST

Expression

Bacterial

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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hROSA26 CRISPR-pX330

Plasmid

#105927

Purpose

hROSA26 targeting CRISPR plasmid

Depositor

Masato Kanemaki

Publication

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hROSA26 gRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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FlagETR3 (CELF2)

Plasmid

#96900

Purpose

expression of Flag-tagged human CELF2 (aka CUGBP2)

Depositor

Thomas Cooper

Publication

Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

Insert

human CELF2 cDNA (CELF2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pNCS YFP

Plasmid

#91759

Purpose

Bacterial expression of a fluorescent protein, Yellow Fluorescent Protein (YFP)

Depositor

Erik Rodriguez, Roger Tsien

Publication

Cubitt et al Trends Biochem Sci. 1995 Nov;20(11):448-

Insert

Venus YFP (A206K)

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Promoter

T7

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-PCAF

Plasmid

#63705

Purpose

retroviral expression of human PCAF in mammalian cells

Depositor

Kai Ge

Publication

Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

Insert

PCAF (KAT2B Human)

Use

Retroviral

Tags

None

Expression

Mammalian

Mutation

E452G

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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THBS1-bio-His

Plasmid

#53417

Purpose

Expresses full-length Thrombospondin-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Publication

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

THBS1 (THBS1 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p201B Cas9

Plasmid

#59177

Purpose

Cas9 driven by double 35S, BAR for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Publication

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
BAR

Use

CRISPR

Promoter

2x35S

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-mCAMK-RFP

Plasmid

#22908

Depositor

Edward Callaway

Publication

Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

Insert

mouse alpha-calcium/calmodulin-dependent protein kinase II promoter (Camk2a Mouse)

Use

AAV

Expression

Mammalian

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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EYFP-Clathrin

Plasmid

#20921

Depositor

Xiaowei Zhuang

Publication

Rust et al Nat Struct Mol Biol. 2004 Jun . 11(6):56

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Clathrin, light polypeptide (Lca) (Clta Mouse)

Tags

EYFP

Expression

Mammalian

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pMD.RVG.CVS24-N2c

Plasmid

#19712

Depositor

Manfred Schubert

Publication

Mentis et al J Neurosci Methods. 2006 Oct 30. 157(2):

Insert

Rabies virus CVS24-N2c glycoprotein

Expression

Mammalian

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3 Flag FKHR H215R

Plasmid

#13509

Depositor

Kunliang Guan

Publication

Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FKHR H215R (FOXO1 Human)

Tags

Flag

Expression

Mammalian

Mutation

H215R

Available since

Dec. 11, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article