19,660 results

1. AAV-hSyn-RiboL1-jGCaMP8m

   Plasmid

   #169248

   Purpose

   AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8m, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8m protein.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Insert

   RiboL1-jGCaMP8m
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV-Sp1

   Plasmid

   #12097

   Depositor

   Robert Tjian

   Insert

   Sp1 (SP1 Human)
   

   Expression

   Mammalian

   Mutation

   aa1-87 deleted

   Available Since

   Aug. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. 7TFP

   Plasmid

   #24308

   Depositor

   Roel Nusse

   Article

   Fuerer et al PLoS One. 2010 . 5(2):e9370.

   Insert

   7xTcf-FFluc // SV40-PuroR
   

   Use

   Lentiviral

   Available Since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQE-60NA-msGFP2

   Plasmid

   #135301

   Purpose

   Encodes a photostable monomeric superfolder GFP

   Depositor

   Benjamin Glick

   Article

   Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

   Insert

   msGFP2
   

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1_nMagHigh1-EGFP-CAAX

   Plasmid

   #67300

   Purpose

   Blue light dependent photoswitching system

   Depositor

   Moritoshi Sato

   Article

   Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

   Insert

   nMagHigh1-EGFP-CAAX
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. GAL4-DrBphP

   Plasmid

   #158986

   Purpose

   Expresses DrBphP fused with an N-terminal GAL4 DNA binding domain in mammalian cells

   Depositor

   Liangcai Gu

   Article

   Huang et al ACS Synth Biol. 2020 Nov 12. doi: 10.102

   Insert

   GAL4-DrBphP
   

   Use

   Lentiviral

   Tags

   Myc tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIS1-Eef25UTR-TOPmut-renilla

   Plasmid

   #38236

   Depositor

   David Sabatini

   Article

   Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

   Insert

   Eef2_TOPm_5UTR/Renilla (Eef2 Mouse)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   The first nucleotides of the 5' UTR were cha…

   Promoter

   Endogenous Eef2

   Available Since

   Nov. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

8. μ2-HA-WT

   Plasmid

   #32752

   Depositor

   Alexander Sorkin

   Article

   Nesterov et al EMBO J. 1999 May 4;18(9):2489-99.

   Insert

   μ2 subunit of clathrin adaptor complex AP-2 (Ap2m1 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDonor14-N7HELIX-KanR

   Plasmid

   #190663

   Purpose

   Kanamycin resistance donor for N7HELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   KanR, R6K origin of replication
   

   Expression

   Bacterial

   Promoter

   NA

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-Puro-TDP-43-A315T

    Plasmid

    #133755

    Purpose

    Lentiviral expression of human TDP-43 A315T disease mutant in mammalian cells.

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Changed alanine 315 to threonine

    Promoter

    CMV

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. SOX2se-CPG-TV

    Plasmid

    #70155

    Purpose

    a targeting vector to target the endogenous Sox2 SE CPG island with Snrp-Tomato-polyA

    Depositor

    Rudolf Jaenisch

    Article

    Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

    Insert

    5'hSOX2se-Snrp-Tomato-polyA-Pgk-PURO-3'hSOX2se
    

    Use

    Mouse Targeting and Synthetic Biology

    Available Since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. eIF2a 2

    Plasmid

    #21808

    Depositor

    David Ron

    Insert

    eIF2a (EIF2S1 Human)
    

    Expression

    Mammalian

    Mutation

    S52A

    Available Since

    Sept. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRsetB-His7tag-Peredox-mCherry

    Plasmid

    #32382

    Depositor

    Gary Yellen

    Article

    Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

    Insert

    His7-Peredox-mCherry
    

    Tags

    His7tag

    Expression

    Bacterial

    Available Since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMCP-RecT

    Plasmid

    #183827

    Purpose

    pLenti-EF1A-MCP-EXTEN-RecT-NLS

    Depositor

    Le Cong

    Article

    Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

    Insert

    MCP-RecT_NLS
    

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3 FlipGFP(Mpro) T2A mCherry

    Plasmid

    #163078

    Purpose

    Expresses FlipGFP-based activity reporter of SARS-CoV-2 main protease Mpro and also mCherry

    Depositor

    Xiaokun Shu

    Article

    Li et al PLoS Pathog. 2021 Sep 3;17(9):e1009898.

    Insert

    FlipGFP-based Mpro activity reporter and mCherry
    

    Expression

    Mammalian

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. LeGO-EBFP2

    Plasmid

    #85213

    Purpose

    Lentiviral vector expressing EBFP2 fluorescent protein driven by SFFV promoter

    Depositor

    Boris Fehse

    Article

    Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

    Insert

    EBFP2
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-RhoA Biosensor

    Plasmid

    #68026

    Purpose

    C-terminus of Anillin in mammalian expression vector pEGFP-C1

    Depositor

    Michael Glotzer

    Article

    Piekny et al Curr Biol. 2008 Jan 8;18(1):30-6. Epub 2

    Insert

    Anillin (AHD+PH) (ANLN Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221_SLC7A5

    Plasmid

    #132197

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC7A5 (SLC7A5 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 28, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pUltra-hot

    Plasmid

    #24130

    Purpose

    3rd generation Lentiviral vector for bi-cistronic expression of mCherry and the gene of interest

    Depositor

    Malcolm Moore

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Feb. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLH-sgRNA1

    Plasmid

    #75388

    Purpose

    sgRNA1

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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