176,063 results
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Plasmid#169815PurposeExpresses codon optimised full length His tagged PARP1 in bacterial cellsDepositorInsertPoly[ADP-ribose] polymerase 1 (PARP1 Human, Codon optimised, Synthetic)
TagsMKHHHHHHMKQExpressionBacterialMutationValine 762 mutated to an alaninePromoterT7 promoterAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-GCaMP6s
Plasmid#171156PurposeEncodes GCaMP6sDepositorInsertGCaMP6s
ExpressionMammalianAvailable sinceAug. 13, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FLX1.8NFATGFPpd2HS4
Plasmid#169096PurposeLentiviral vector encoding a short-lived GFP under the transcriptional control of a mutlimerized NFAT gene enhancer to measure T cell activationDepositorInsertsNFAT enhancer (from IL-4 promoter)
Destabilized green fluorescent protein
UseLentiviralPromoterNFAT enhancer + synthetic TATA boxAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS2M-PH-gagpol-D64V
Plasmid#166031PurposeExpression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at N terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.DepositorInsertMS2-Gag-Pol
UseLentiviralMutationD64V mutation within the integrase within PolAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LifeAct-mGreenLantern
Plasmid#164459PurposeLocalization of mGreenLantern fluorescent protein to actinDepositorInsertmGreenLantern
TagsLifeActExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-Sfp
Plasmid#159617PurposeExpression plasmid for Sfp phosphopantetheinyl transferaseDepositorInsertSfp
Tags6HISExpressionBacterialAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFJ2474 - pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
Plasmid#159816PurposeOptimized enzyme for genome editing in C. elegansDepositorInsertpEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
ExpressionWormMutationNot applicableAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE
Plasmid#162382PurposeAAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expressionDepositorHas serviceAAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8f
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationQ315LPromoterCAGAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-mem-TurboID
Plasmid#149409PurposeMammalian expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in mammalian expression plasmid. Amp/Neo selectionDepositorInsertmem-TurboID
TagsHA and V5ExpressionMammalianPromoterCMVAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-PE2-SpRY
Plasmid#159979PurposePrime editing in mammalian cellsDepositorInsertPE2-SpRY
TagsBpSV40 NLSExpressionMammalianPromoterCMVAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV-Tol2 transposase
Plasmid#158774PurposeExpression of Tol2 transposaseDepositorInsertTol2 tranposase
ExpressionMammalianMutationTol2 codon usage frequency well suitable for muri…Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-tdTomato
Plasmid#159275PurposeHuman AAVS1 targeting vector for knockin of a CAGGS promoter-driven tdTomato cassette (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.DepositorInserttdTomato
UseAAV; S1 knockin donor vectorExpressionMammalianPromoterCAGGSAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV-loxp-DsRed-loxp-eGFP
Plasmid#141148Purposelentiviral expression vector to generate a hypoxia fate-mapping systemDepositorInsertloxp-DsRed-loxp-eGFP
UseLentiviralAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOPINO_NbH11-H4
Plasmid#155364PurposeExpresses nanobody H11-H4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2DepositorInsertH11-H4
TagsHis6ExpressionBacterialPromoterT7Available sinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
MLRV2: NF-kb-SMAD-DBE-P53-AP1
Plasmid#124536PurposeMultiplex luciferase reporter vector, with luciferase reporters for the pathways NFKB, SMAD, FOXO, P53 and AP1DepositorInserts5 copies of the NF-kb DNA binding motif
7xSMAD_RE::FLuc
3xDBE_BE::Renilla (FOXO)
2xP53_RE::NLuc
6xAP-1_RE::GrRenilla
UseLuciferase and Synthetic BiologyExpressionMammalianAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
tet_pLKO.1_puro_shNRF2 #1
Plasmid#136584PurposeExpresses an inducible short hairpin targeting human NRF2 sequenceDepositorAvailable sinceJune 13, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV FLEX Synaptophysin GFP
Plasmid#137188PurposeCre-dependent expression of synaptophysin-GFPDepositorInsertSynaptophysin GFP (Syp Mouse)
UseAAVAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCEP4-myc-HLA-A2
Plasmid#135504PurposeMammalian expression of myc-tagged HLA-A*02:01DepositorInsertHLA-A*02:01 (HLA-A Human)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GRAB_ACh3.0
Plasmid#121923Purposeexpress the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0 in a cre-dependent mannerDepositorInsertGPCR activation based ACh sensor GRAB-ACh3.0
UseAAVPromoterhSynAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
NW50
Plasmid#133445PurposeCovalently circularized nanodisc NW50 for studying membrane proteins and viral entry.DepositorInsertNW50
ExpressionBacterialAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only