We narrowed to 13,276 results for: cDNA

1. pDEST40-2XFL-Src-KD

   Plasmid

   #140313

   Purpose

   Expresses Flag-tagged human Src-KD in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src mutant (SRC Human)
   

   Use

   Tags

   2X Flag

   Expression

   Mammalian

   Mutation

   Contains only the KD region

   Promoter

   CMV

   Available Since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST47-ARL8B

   Plasmid

   #67467

   Purpose

   Mammalian expression of hArl8b with stop codon (native expression)

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL8B (ARL8B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

3. Beta2-AR-TYY

   Plasmid

   #32694

   Depositor

   Robert Lefkowitz

   Article

   Shenoy et al J Biol Chem. 2006 Jan 13;281(2):1261-73.

   Insert

   β2AR (ADRB2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   T68F, Y132G Y219A

   Promoter

   CMV

   Available Since

   Nov. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pDEST40-2XFL-Src-Y419F

   Plasmid

   #140317

   Purpose

   Expresses Flag-tagged human Src-Y419F in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src mutant (SRC Human)
   

   Use

   Tags

   2X Flag

   Expression

   Mammalian

   Mutation

   Y419F

   Promoter

   CMV

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. NSP14-Flag

   Plasmid

   #210346

   Purpose

   Expresses SARS-CoV-2 NSP14 fused with Flag in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-CoV-2 NSP14 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST47-ARL6-GFP

   Plasmid

   #67401

   Purpose

   Mammalian expression of hArl6 with C-term GFP tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL6 (ARL6 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pTE4254

   Plasmid

   #89050

   Purpose

   Expresses St chimeric gRNA and human codon-optimized StCas9

   Depositor

   Ervin Welker

   Article

   Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

   Inserts

   St chimeric gRNA
   hStCas9
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and human U6

   Available Since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTE3646

   Plasmid

   #137714

   Purpose

   Expresses LbCas12a RVRR variant in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

   Insert

   LbCas12a-RVRR
   

   Use

   Tags

   3xHA, NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   G532R, K538V, Y542R, K595R

   Promoter

   CMV

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SpyCas9-AIDx

   Plasmid

   #135343

   Purpose

   Expression of wildtype nSpyCas9 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSpyCas9-hAIDx
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. B*58:02:01

    Plasmid

    #203262

    Purpose

    Overexpress HLA allele

    Depositor

    Catherine Wu, Derin Keskin

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    B*58:02:01 (HLA-B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Opto-GPR3

    Plasmid

    #106000

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR3; Opto-GPR3; A2)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR3 (GPR3 Human, Bovine)
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. bArr1-ALFA

    Plasmid

    #201496

    Purpose

    Expression of the beta-arrestin1 containing a C-terminal ALFA tag.

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    bArr1-ALFA (ARRB1 Human)
    

    Use

    Tags

    ALFA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. nLuc_AP10_PPVs_P9mS

    Plasmid

    #119302

    Purpose

    Expresses N-terminus of split luciferase fused to antiparallel coiled-coil AP10 connected with autoinhibitory coil P9mS and PPVs cleavage site in antiparallel harpin linker/for SPOC logic

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split nLuc fused to antiparallel Coiled-coil AP10 connected with P9mS via PPV cleavable linker
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mH2A2-CT-MYC

    Plasmid

    #45170

    Depositor

    Brian Chadwick, Hunt Willard

    Article

    Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

    Insert

    H2Afy2 (MACROH2A2, H2AFY2 Human)
    

    Use

    Tags

    HIS and MYC

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

15. LRRK2-K1906M

    Plasmid

    #35946

    Depositor

    Zhaoyang Feng

    Article

    Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

16. FN-Ets2

    Plasmid

    #44603

    Depositor

    Robert Oshima

    Article

    Galang et al J Biol Chem. 1996 Apr 5;271(14):7992-8.

    Insert

    Ets2 (Ets2 Mouse)
    

    Use

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Erk2

    Plasmid

    #40777

    Purpose

    Expression of rat Erk2 in mammalian cells

    Depositor

    Dustin Maly

    Article

    Hari et al Chem Biol. 2014 May 22;21(5):628-35. doi

    Insert

    Erk2 (Mapk1 Rattus norvegicus)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Starting Met removed

    Promoter

    CMV

    Available Since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pDEST40-ARF6-V5-His6

    Plasmid

    #67436

    Purpose

    Mammalian expression of hArf6 with C-term V5 and His6 tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF6 (ARF6 Human)
    

    Use

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

19. CMV:: HA-hM4Dnrxn

    Plasmid

    #52525

    Purpose

    Expresses N-terminal HA tagged hM4Dnrxn

    Depositor

    Scott Sternson

    Article

    Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

    Insert

    HA-hM4Dnrxn (CHRM4 Human, Synthetic)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. BCL9-BirA*

    Plasmid

    #109147

    Purpose

    To generate stable cell lines expressing BCL9-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cells

    Depositor

    Mariann Bienz

    Article

    van Tienen et al Elife. 2017 Mar 15;6. doi: 10.7554/eLife

    Insert

    BCL9 (BCL9 Human)
    

    Use

    Tags

    BirA(R118G)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO2

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. LIC-Z

    Plasmid

    #153543

    Purpose

    perform light induced optogenetic clustering of TCR ζ-Chain as described in the associated publication

    Depositor

    Katharina Gaus

    Article

    Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

    Insert

    human TCR ζ-Chain (CD247 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    CMV

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKK-HA-TEV

    Plasmid

    #105769

    Purpose

    Expression of your protein of interest in fusion with HA at the N-terminus. The tag is cleavable by TEV protease.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    HA-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ABEMax nSaCas9

    Plasmid

    #135364

    Purpose

    Expresion of SaCas9 nickase (D10A) with ABEMax at the N terminus

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    ABEMax nSaCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pKK-TEV-HA

    Plasmid

    #105785

    Purpose

    Expression of your protein of interest in fusion with HA at the C-terminus. The tag is cleavable by TEV protease.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. MCP-mCherry-SEC61B

    Plasmid

    #207671

    Purpose

    To express fluorescently labeled MS2 coat binding protein (MCP) fused with ER resident protein SEC61B

    Depositor

    Christine Mayr

    Article

    Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

    Insert

    SEC61B (SEC61B Human)
    

    Use

    Tags

    MCP-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. F-MED28

    Plasmid

    #15368

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al Mol Cell. 2004 Jun 4. 14(5):685-91.

    Insert

    MED28 (MED28 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

27. pDEST40-2XFL-Src-Y530EEI

    Plasmid

    #140323

    Purpose

    Expresses Flag-tagged human Src-Y530EEI in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    Y530EEI

    Promoter

    CMV

    Available Since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSBbiRP Hu Full Length CD142 (Tissue Factor; F3)

    Plasmid

    #183254

    Purpose

    Expression of full length human tissue factor (CD142; F3) in a Sleeping Beauty transposon vector

    Depositor

    Alexander McLellan

    Article

    Muhsin-Sharafaldine et al Oncotarget. 2016 Aug 30;7(35):56279-5629

    Insert

    Tissue factor (F3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pmyc-GFP-TNRC6A-(935-1026)-NES-mut

    Plasmid

    #42021

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    contain aa935-1026, F951A, F955A, I958A, F960A

    Promoter

    CMV

    Available Since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Kif1A(1-489)-DHFR-myc

    Plasmid

    #117833

    Purpose

    Constitutively active Kinesin-3 motor tagged with DHFR and myc

    Depositor

    Thomas Schwarz

    Article

    Gutnick et al Nat Cell Biol. 2019 Jun;21(6):768-777. d

    Insert

    Kif1A (1-489) (Kif1a Mouse)
    

    Use

    Tags

    DHFR and myc

    Expression

    Mutation

    only aa 1-489 (motor domain)

    Promoter

    CMV

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge