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Showing: 121 - 160 of 3512 results

  2. pLCHKO_TK1_HPRT1_Lb_3

    Plasmid
    #155061
    Purpose
    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155061-plasmid-map-sequence-id-308580

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  3. pAAV CAG Barcode2

    Plasmid
    #229063
    Purpose
    Expression mapping
    Depositor
    Scott Sternson
    Insert
    CAG Barcode2
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    229063-plasmid-map-sequence-id-455086

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  4. pUDP123

    Plasmid
    #107269
    Purpose
    pUDP123 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes OpADE2 and OpNIAD and Spcas9D147Y P411T in O. parapolymorpha (HH-gRNAOpADE2-HDV-linker-HH-gRNAOpNIAD-HDV)
    Depositor
    Jean-Marc Daran
    Article
    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4
    Insert
    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting OpADE2 and NIAD in O. parapolymorpha
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    107269-plasmid-map-sequence-id-208617

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  10. pAW218

    Plasmid
    #113240
    Purpose
    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader IHF binding site flip
    Depositor
    Jennifer Doudna
    Article
    Wright et al Science. 2017 Sep 15;357(6356):1113-1118
    Inserts
    Cas1
    Cas2
    Leader-repeat
    Use
    Tags
    Expression
    Bacterial
    Mutation
    AAAAAATCATTAATTAATAATAGGTTATG->CATAACCTATTATTA…
    Promoter
    Available since
    Aug. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    113240-plasmid-map-sequence-id-217755

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  13. pSC2

    Plasmid
    #91180
    Purpose
    T-DNA vector for combinatorial deletion of NCR genes in medicago truncatula (Csy4 array of 6 gRNAs under the control of CmYLCV promoter)
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    gRNAs targeting NCR genes
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91180-plasmid-map-sequence-id-182720

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  14. pATZ-BFP-HA-GFP11

    Plasmid
    #177721
    Purpose
    ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to blue fluorescence protein (BFP) tag protein, an HA tag and the eleventh β-strand of GFP
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    alpha-1-antitrypsin (SERPINA1 Human)
    Use
    Tags
    GFP11, HA, and TagBFP
    Expression
    Bacterial and Mammalian
    Mutation
    E342K (ATZ mutant)
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177721-plasmid-map-sequence-id-352621

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  15. pJ137

    Plasmid
    #29487
    Depositor
    Jonathan Tait
    Article
    Jin et al J Biol Chem 2004; 279:40351-57.
    Insert
    annexin V-128 (ANXA5 Human)
    Use
    Tags
    (Met)-Ala-Gly-Gly-Cys-Gly-His
    Expression
    Bacterial
    Mutation
    Deletion of the initiator Met at position 1 of wi…
    Promoter
    Available since
    May 17, 2011
    Availability
    Academic Institutions and Nonprofits only

  16. pJ145

    Plasmid
    #29491
    Depositor
    Jonathan Tait
    Article
    Jin et al J Biol Chem 2004; 279:40351-57.
    Insert
    annexin V-128 (ANXA5 Human)
    Use
    Tags
    (Met)-Ala-Gly-Gly-Cys-Gly-His
    Expression
    Bacterial
    Mutation
    Deletion of the initiator Met at position 1 of wi…
    Promoter
    Available since
    June 7, 2011
    Availability
    Academic Institutions and Nonprofits only

  17. pQCXIP-SHMY-A1Pi

    Plasmid
    #182648
    Purpose
    Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), one tyrosine sulfation (TS) motif (Y), myc (M), His6 (H) and A1Pi sequence
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    A1Pi (SERPINA1 Human)
    Use
    Retroviral
    Tags
    His6-tag, N-terminal ER targeting sequence of hem…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182648-plasmid-map-sequence-id-358630

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  18. pJ139

    Plasmid
    #29489
    Depositor
    Jonathan Tait
    Article
    Jin et al J Biol Chem 2004; 279:40351-57.
    Insert
    annexin V-128 (ANXA5 Human)
    Use
    Tags
    (Met)-Ala-Gly-Gly-Cys-Gly-His
    Expression
    Bacterial
    Mutation
    Deletion of the initiator Met at position 1 of wi…
    Promoter
    Available since
    June 6, 2011
    Availability
    Academic Institutions and Nonprofits only

  19. pJ143

    Plasmid
    #29490
    Depositor
    Jonathan Tait
    Article
    Jin et al J Biol Chem 2004; 279:40351-57.
    Insert
    annexin V-128 (ANXA5 Human)
    Use
    Tags
    (Met)-Ala-Gly-Gly-Cys-Gly-His
    Expression
    Bacterial
    Mutation
    Deletion of the initiator Met at position 1 of wi…
    Promoter
    Available since
    June 6, 2011
    Availability
    Academic Institutions and Nonprofits only

  20. pAAV syn Barcode1

    Plasmid
    #229062
    Purpose
    Expression mapping
    Depositor
    Scott Sternson
    Insert
    Syn Barcode1
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    229062-plasmid-map-sequence-id-454825

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  21. pAAV Syn Barcode19

    Plasmid
    #226196
    Purpose
    Expression mapping
    Depositor
    Scott Sternson
    Insert
    Syn Barcode19
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226196-plasmid-map-sequence-id-447886

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  22. pAAV Syn Barcode22

    Plasmid
    #226193
    Purpose
    Expression mapping
    Depositor
    Scott Sternson
    Insert
    Syn Barcode22
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226193-plasmid-map-sequence-id-447883

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  25. pWT029h

    Plasmid
    #96861
    Purpose
    (d)guanine-agRNA (GFP activation,18 nt blocker with bulges)
    Depositor
    David Liu
    Article
    Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10
    Insert
    (d)guanine-agRNA (GFP activation,18 nt blocker with bulges)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    96861-plasmid-map-sequence-id-189275

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  26. pWT049e

    Plasmid
    #96863
    Purpose
    (d)guanine-agRNA (19 nt blocker with bulges, RFP activation)
    Depositor
    David Liu
    Article
    Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10
    Insert
    (d)guanine-agRNA (19 nt blocker with bulges, RFP activation)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    96863-plasmid-map-sequence-id-189252

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  31. pUDP010

    Plasmid
    #101166
    Purpose
    E. coli/S. cerevisiae amdS shuttle vector expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 in S. pastorianus (HH-gRNASeILV6-HDV)
    Depositor
    Jean-Marc Daran
    Article
    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.
    Insert
    HH-gRNA-HDV targetting SeILV6 in S. pastorianus
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101166-plasmid-map-sequence-id-199274

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  33. pUDP012

    Plasmid
    #101167
    Purpose
    E. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y P411T and expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV)
    Depositor
    Jean-Marc Daran
    Article
    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.
    Insert
    HH-gRNA-HDV targetting SeILV6 in S. pastorianus
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101167-plasmid-map-sequence-id-199268

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  39. pLCHKO_MDM4_exon_deletion_1

    Plasmid
    #155073
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155073-plasmid-map-sequence-id-308586

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  40. pUDP025

    Plasmid
    #103874
    Purpose
    Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces lactis
    Depositor
    Jean-Marc Daran
    Article
    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4
    Insert
    HH-gRNA-HDV targetting ADE2 in Kluyveromyces lactis
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    103874-plasmid-map-sequence-id-199263

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Showing: 121 - 160 of 3512 results