We narrowed to 772 results for: tev cleavage
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Plasmid#45580DepositorUseAAV; Adeno associated viral vectorTagsExpressionMutationLinker replaced with a TEV protease cleavage sitePromoterEF1aAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only
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pGEX2T-TEV CHMP1B 4-178
Plasmid#108284PurposeExpresses residues 4-178 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-279DepositorInsertCHMP1B (CHMP1B Human)
UseTagsGSTExpressionBacterialMutationdeleted amino acids 1-3 and 179-199PromoterAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T-TEV CHMP1B 1-143
Plasmid#108286PurposeExpresses residues 1-143 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-278DepositorInsertCHMP1B (CHMP1B Human)
UseTagsGSTExpressionBacterialMutationamino acids 1-143PromoterAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-TEV-KRAS
Plasmid#111849PurposeExpresses wild type human KRAS (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS (KRAS Human)
UseTags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationPromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet-6xHis-TEV-Nsp8(76-198)
Plasmid#201024PurposeBacterial expression of codon optimized SARS-CoV-2 nsp8 residues 76-198, with an N-terminus His tag followed by a TEV cleavage site.DepositorInsertnon-structural protein 8 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
UseTags6xHis-TEVExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
6xHis-TEV-ATG3 (Human autophagy E2-like enzyme)
Plasmid#169079PurposeHuman ATG3DepositorInsertATG3 (ATG3 Human)
UseTags6X Histidine Tag, TEV cleavage siteExpressionBacterialMutationPromoterT7 lac promoterAvailable sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP TEV HDAC8_374
Plasmid#122174PurposeExpresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tagDepositorInsertHis6 MBP TEV HDAC8_374 (HDAC8 Human)
UseTagshexahistidine tag and tobacco etch virus protease…ExpressionBacterialMutationResidues Met1–Pro7 and H375-V377 have been delete…PromoterT7Available sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mIRFP670nuc-TEV-Xph20-ETWV
Plasmid#133044PurposeExpresses Xph20-ETWV in mammalian cells with nuclear miRFP670 as reporterDepositorInsertXph20-ETWV
UseTagsNLS, TEV protease + TEV cleavage site, and miRFP6…ExpressionMammalianMutationS63KPromoterCAGAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T-TEV CHMP1B 186-199
Plasmid#108285PurposeExpresses residues 186-199 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-283DepositorInsertCHMP1B (CHMP1B Human)
UseTagsGSTExpressionBacterialMutationamino acids 186-199PromoterAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T-TEV CHMP1B 144-178
Plasmid#108287PurposeExpresses residues 144-178 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-281DepositorInsertCHMP1B (CHMP1B Human)
UseTagsGSTExpressionBacterialMutationamino acids 144-178PromoterAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-TEV-KRAS G12V
Plasmid#111850PurposeExpresses activated human KRAS G12V (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS G12V (KRAS Human)
UseTags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationChanged Glycine 12 to ValinePromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-TEV-KRAS G13D
Plasmid#111851PurposeExpresses activated human KRAS G13D (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS G13D (KRAS Human)
UseTags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationChanged Glycine 13 to Aspartic AcidPromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T-TEV CHMP1B 4-199 L188A/L192A
Plasmid#108289PurposeExpresses residues 4-199 with L to A mutations at residues 188 and 192 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-284DepositorInsertCHMP1B (CHMP1B Human)
UseTagsGSTExpressionBacterialMutationchanged L 188 to A, changed L 192 to APromoterAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T-TEV CHMP1B 4-199 L192A/L195A
Plasmid#108291PurposeExpresses residues 4-199 with L to A mutations at residues 192 and 195 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-285DepositorInsertCHMP1B (CHMP1B Human)
UseTagsGSTExpressionBacterialMutationchanged L 192 to A, changed L 195 to APromoterAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T-TEV CHMP1B 186-199 L188A/L192A
Plasmid#108288PurposeExpresses residues 186-199 with L to A mutations at residues 188 and 192 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-286DepositorInsertCHMP1B (CHMP1B Human)
UseTagsGSTExpressionBacterialMutationchanged L 188 to A, changed L 192 to APromoterAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T-TEV CHMP1B 186-199 L192A/L195A
Plasmid#108290PurposeExpresses residues 186-199 with L to A mutations at residues 192 and 195 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-287DepositorInsertCHMP1B (CHMP1B Human)
UseTagsGSTExpressionBacterialMutationchanged L 188 to A, changed L 192 to APromoterAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-FLAG-TEV-KRAS G12C
Plasmid#111848PurposeExpresses activated human KRAS G12C (amino acids 1 - 169) in E. coli with amino terminal 6xHIS and FLAG tags that can be removed with TEV protease.DepositorInsertKRAS G12C (KRAS Human)
UseTags6xHis-tag, FLAG-tag, and TEV protease cleavage se…ExpressionBacterialMutationChanged Glycine 12 to CysteinePromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_His-TEV-EiCsm6
Plasmid#172487PurposeBacterial expression of codon-optimized His-EiCsm6 with a TEV protease cleavage site.DepositorInsertEnterococcus italicus Csm6 (codon optimized)
UseTagsHis6ExpressionBacterialMutationPromoterT7 promoter and lac operatorAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET_His6-SUMO-TEV-TtCsm6
Plasmid#172486PurposeBacterial expression of codon-optimized His-SUMO-TtCsm6 with a TEV protease cleavage site.DepositorInsertT. thermophilus Csm6 (codon optimized) (csm6 Synthetic)
UseTagsHis-SUMO-TEVExpressionBacterialMutationPromoterT7 promoter and lac operatorAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis-TEV-mCherry-LC3B-Gly (Microtubule-associated proteins 1A/1B light chain 3B)
Plasmid#169168PurposeConjugatable form of LC3B lacking 5 C-terminal amino acids (MKLSV), with C-terminal Glycine120 exposed for lipidation reaction.DepositorInsertMAP1LC3B (MAP1LC3B Human)
UseTags6X Histidine Tag, TEV cleavage site, mCherryExpressionBacterialMutationPromoterT7 lac promoterAvailable sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
His-ZZ-TEV-SNAPf DHC1_IC2C_LIC2_Tctex1_Robl1_LC8
Plasmid#111903PurposeFull length human cytoplasmic dynein 1 complex, optimised for Sf9 expression. 8xHis-ZZ tag followed by TEV cleavage site and SNAPf tag on the N-terminus of dynein heavy chain 1.DepositorInsertsUseTags8xHis, SNAPf-tag, TEV site, and ZZ-tagExpressionInsectMutationOptimised for expression in Sf9 cells.PromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
CPH3566_GST-TEV-Spt6p-tSH2-1223-1451
Plasmid#227085PurposeBacterial expression of GST-tagged Spt6 tSH2 domain (1223-1451)DepositorInsertspt6-tsh2 (SPT6 Budding Yeast)
UseTagsGST-TEV_cleavage_siteExpressionBacterialMutationPromoterT7Available sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
CPH3737_GST-TEV-Spt6p-tSH2-1223-1453
Plasmid#227087PurposeBacterial expression of K1355A/K1435A double-mutant GST-tagged Spt6 tSH2 domain (1223-1453)DepositorInsertspt6-tsh2 (SPT6 Budding Yeast)
UseTagsGST-TEV_cleavage_siteExpressionBacterialMutationK1355A, K1435APromoterT7Available sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
CPH3747_GST-TEV-Spt6p-tSH2-1223-1452
Plasmid#227086PurposeBacterial expression of R1282H single-mutant GST-tagged Spt6 tSH2 domain (1223-1452)DepositorInsertspt6-tsh2 (SPT6 Budding Yeast)
UseTagsGST-TEV_cleavage_siteExpressionBacterialMutationR1282HPromoterT7Available sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
CPH3736_GST-TEV-Spt6p-tSH2-1223-1454
Plasmid#227088PurposeBacterial expression of R1282H/K1355A/K1435A triple-mutant GST-tagged Spt6 tSH2 domain (1223-1454)DepositorInsertspt6-tsh2 (SPT6 Budding Yeast)
UseTagsGST-TEV_cleavage_siteExpressionBacterialMutationR1282H, K1355A, K1435APromoterT7Available sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
CPH3949_HIS-MBP-TEV-Tom1p-HECT-2850-3268
Plasmid#227089PurposeBacterial expression of HIS-MBP-TEV tagged Tom1p HECT domain (2850-3268)DepositorInserttom1p-HECT (TOM1 Budding Yeast)
UseTagsHIS-MBP-TEV_cleavage_siteExpressionBacterialMutationPromoterT7Available sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-BN-rNLRP1(S969A)-FLAG
Plasmid#217324PurposePurification of autocleavage-deficient Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cellsDepositorInsertNLRP1
UseTagsFLAG and His-TEVExpressionInsectMutationS969APromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-avi-TEV-LIC_DCAF12:1-453
Plasmid#210940PurposeInsect expression of full-length human DCAF12. N-terminal 6X His tag, biotin acceptor peptide tag and TEV cleavage site.DepositorInsertDCAF12:M1-S453 (DCAF12 Human)
UseTagsExpressionInsectMutationR131Q natural variant (VAR_035322)PromoterPolyhedrinAvailable sinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBac HTB His-TEV-LEW-rNLRP1(S969A)-FLAG
Plasmid#217321PurposePurification of autocleavage-deficient Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cellsDepositorInsertNLRP1
UseTagsFLAG and His-TEVExpressionInsectMutationS969APromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-MKH8SUMO
Plasmid#79526PurposeBacterial expression plasmid used for T7 promoter driven expression of recombinant proteins with N-terminal 8X His, thrombin cleavage site, SUMO, and TEV cleavage site.DepositorTypeEmpty backboneUseTags8xHis-Thrombin-SUMO-TEV and TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceJune 16, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
371-B
Plasmid#97027PurposeHis6-tev-yORFDepositorTypeEmpty backboneUseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
371-GFP
Plasmid#97030PurposeHis6-GFP-tev-yORFDepositorTypeEmpty backboneUseTagsGFP fusion, His6 tag, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
371-Bc
Plasmid#97028PurposeyORF-tev-His6DepositorTypeEmpty backboneUseTagsHis6 tag and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
371-T
Plasmid#97035PurposeHis6-Thioredoxin-tev-yORFDepositorTypeEmpty backboneUseTagsHis6 tag, TEV cleavage site, and Thioredoxin funs…ExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
371-R
Plasmid#97033PurposeStrepII-tev-yORFDepositorTypeEmpty backboneUseTagsStrepII tag and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
371-S
Plasmid#97034PurposeHis6-Sumo-tev-yORFDepositorTypeEmpty backboneUseTagsHis6 tag, SUMO domain, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
371-O
Plasmid#97032PurposeHis6-Mocr-tev-yORFDepositorTypeEmpty backboneUseTagsHis6 tag, TEV cleavage site, and mOCR fusionExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
371-L
Plasmid#97031PurposeFLAG-tev-yORFDepositorTypeEmpty backboneUseTagsFLAG tag and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
371-C
Plasmid#97029PurposeHis6-MBP-N10-tev-yORFDepositorTypeEmpty backboneUseTagsHis6 tag, MBP domain, N10 linker, and TEV cleavag…ExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1_OCT4_S235A
Plasmid#40639DepositorInsertPOU5F1 (POU5F1 Human)
UseTagsGST, TEV Cleavage, TEV cleavage, Thrombin Cleavag…ExpressionBacterialMutationS235APromotertacAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only