We narrowed to 8,631 results for: sgrna

1. pCnCas12f1HS_sgRNA_MS13_empty

   Plasmid

   #204999

   Purpose

   CnCas12f1 with MS13 sgRNA site for Human cell genome editing

   Depositor

   Quanjiang Ji

   Article

   Su et al Nat Chem Biol. 2024 Feb;20(2):180-189. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PtPuc3_diaCas9_sgRNA

   Plasmid

   #109219

   Purpose

   Episome based vector with CRISPR/Cas9_sgRNA module for genome editing in Phaeodactylum tricornutum

   Depositor

   Per Winge

   Article

   Sharma et al Sci Rep. 2018 Sep 26;8(1):14401. doi: 10

   Inserts

   LHCF2 promoter
   diaCas9
   LHCF1 terminator
   U6 promoter
   sgRNA
   U6 3' region
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   LHCF1 terminator, LHCF2 promoter, U6 3' regi…

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pColE1_sgRNA_NT_AmpR

   Plasmid

   #119144

   Purpose

   Encodes non-targeting sgRNA

   Depositor

   Vincent Noireaux

   Article

   Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

   Insert

   non-targeting sgRNA
   

   Use

   Crispr

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pColE1_sgRNA_pos8_AmpR

   Plasmid

   #119151

   Purpose

   Encodes sgRNA targeting position 8 in pColE1_70a_deGFP_KanR

   Depositor

   Vincent Noireaux

   Article

   Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

   Insert

   sgRNA targeting position 8 in pColE1_70a_deGFP_KanR
   

   Use

   Crispr

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. U6>sgRNA(F+E)

   Plasmid

   #59986

   Purpose

   Empty vector for sgRNA cloning (F+E modified backbone)

   Depositor

   Lionel Christiaen

   Article

   Stolfi et al Development. 2014 Nov;141(21):4115-20. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ciinte.U6

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. SaCas9_sgRNA_expression_in_pBluescript

   Plasmid

   #122090

   Purpose

   U6 driven SaCas9 sgRNA expression vector for cloning own guides

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SaCas9 tracr (NEWENTRY S. pyogenes, Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. px458_2A_GFP_sgRNA_TIA1

   Plasmid

   #106097

   Purpose

   CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting TIA1

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

   Insert

   gRNA TIA1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. sgRNA.SFFV.tBFP

   Plasmid

   #169940

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and tBFP from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.

   Depositor

   Benjamin Ebert

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS1_sgRNA

   Plasmid

   #100554

   Purpose

   Expresses AAVS1 sgRNA. Target sequence: GGGGCCACTAGGGACAGGAT

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

   Insert

   AAVS1 sgRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLCKO_Luciferase_sgRNA

    Plasmid

    #74190

    Purpose

    lentiviral vector expressing sgRNA targeting Luciferase

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    Luciferase sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. px458_2A_GFP_sgRNA_RACK1

    Plasmid

    #127123

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    gRNA RACK1 (RACK1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. EC2_4_dCas9_VPR_sgRNA

    Plasmid

    #163709

    Purpose

    Yeast low copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Inserts

    dCas9
    VPR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Nme2sgRNA_pLKO

    Plasmid

    #119926

    Purpose

    Nme2Cas9 U6-driven sgRNA cassette

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    NmeCas9 sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti_sgRNA_MS2_neo

    Plasmid

    #118650

    Purpose

    To clone sgRNA for activation dCas9

    Depositor

    Christopher Vakoc

    Article

    Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. M-NM-sgRNA

    Plasmid

    #48673

    Purpose

    Mammalian U6-driven sgRNA (NMm1) targeting GTCCCCTCCACCCCACAGTG

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with N. meningitidis Cas9, hU6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available Since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. px458_2A_GFP_sgRNA_PELO

    Plasmid

    #127124

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    gRNA PELO (PELO Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. LCV2_mCherry_AAVS1_sgRNA_2

    Plasmid

    #155105

    Purpose

    lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    AAVS1_sgRNA_2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. EC2_3_dCas9_Mxi1_sgRNA

    Plasmid

    #163708

    Purpose

    Yeast low copy plasmid with dCas9, sgRNA expression cassette and Mxi1 transcriptional repressor

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Inserts

    dCas9
    Mxi1+SV40 NLS
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCas9_sgRNA_0

    Plasmid

    #70763

    Purpose

    expresses Ustilago maydis codon-optimized Cas9, contains U. maydis U6 promoter, is self-replicating

    Depositor

    Regine Kahmann

    Article

    Schuster et al Fungal Genet Biol. 2015 Sep 11. pii: S10

    Inserts

    U6 promoter
    cas9
    tnos terminator
    

    Use

    Self-replicating in ustilago maydis, conferring c…

    Tags

    N-terminal NLS, C-terminal HA-tag +NLS

    Expression

    Mutation

    Streptococcus pyogenes gene codon-optimized for U…

    Promoter

    U6 from Ustilago maydis and synthetic otef promot…

    Available Since

    Nov. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. SpCas9_sgRNA_expression_in_pBluescript

    Plasmid

    #122089

    Purpose

    U6 driven SpCas9 sgRNA expression vector for cloning own guides

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SpCas9 tracr (NEWENTRY S. pyogenes, Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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