163,606 results

1. pMOD_A0000

   Plasmid

   #90997

   Purpose

   Module A, Promoter: none, Gene: none, Terminator: none

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Type

   Empty backbone

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. mouse Clock delta 19

   Plasmid

   #177312

   Purpose

   expression of mutant mouse clock

   Depositor

   Joseph Takahashi

   Article

   King et al Cell. 1997 May 16;89(4):641-53. doi: 10.

   Insert

   mouse clock delta 19
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deletion from original mClock gene (514-564aa)

   Promoter

   CMV

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKAR5

   Plasmid

   #149461

   Purpose

   Broad-Host range expression vector, RK2 and pUC origin, AraC activator/repressor, PBAD promoter, mRFP, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   AraC-mRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV Myc rat SAPAP2

   Plasmid

   #40216

   Depositor

   Yoshimi Takai, Yutaka Hata

   Article

   Takeuchi et al J Biol Chem. 1997 May 2;272(18):11943-51

   Insert

   SAPAP2 (Dlgap2 Rat)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   H137Q and K806R

   Promoter

   CMV

   Available Since

   Nov. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

5. TFORF2950

   Plasmid

   #144426

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   MAFB (MAFB Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2+-HypocratesCS

   Plasmid

   #161960

   Purpose

   Mammalian expression of non-targeted inactivated control version of genetically encoded biosensor for (pseudo)hypohalous acids and their derivatives

   Depositor

   Dmitry Bilan

   Article

   Kostyuk et al Nat Commun. 2022 Jan 10;13(1):171. doi:

   Insert

   Hypocrates
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Cysteine 355 to Serine

   Promoter

   CMV, SP6

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIC247

   Plasmid

   #69741

   Purpose

   Retroviral expression of Borealin

   Depositor

   Iain Cheeseman

   Insert

   Borealin (CDCA8 Human)
   

   Use

   Retroviral

   Tags

   eYFP-TEV-S

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pMXs-Rex1

   Plasmid

   #13368

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

   Insert

   Zinc finger protein 42 (Zfp42 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available Since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pMJA167

   Plasmid

   #128536

   Purpose

   Expresses DsRed

   Depositor

   Marcos Alcocer

   Article

   Wang et al Mol Biotechnol. 2014 Feb;56(2):136-46. d

   Insert

   NFAT-DsRed reporter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40-NFAT

   Available Since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-CMV-PtenC124S-Puro

    Plasmid

    #135677

    Purpose

    Constitutive expression (cMV promoter) of C124S mutant Pten cDNA, Puromycin selection

    Depositor

    Florian Karreth

    Insert

    Pten (Pten Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    C124S

    Promoter

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP KNL1 wt hardened and RVSF->AAAA

    Plasmid

    #45225

    Depositor

    Iain Cheeseman, Michael Lampson

    Article

    Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

    Insert

    GFP KNL1 with RVSF-AAAA mutation, resistant to KNL1 siRNA (KNL1 Human)
    

    Use

    Tags

    Expression

    Mutation

    RVSF mutated to AAAA

    Promoter

    Available Since

    May 31, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pSH-EFIRES-P-Seipin-miniIAA7-mCherry

    Plasmid

    #129720

    Purpose

    AAVS1 safe harbor targeting vector expressing miniIAA7-mCherry tagged Seipin

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    Seipin (BSCL2 Human)
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    miniIAA7-mCherry

    Expression

    Mammalian

    Mutation

    silent mutations

    Promoter

    EF1a

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. EIF2AK2 gRNA (BRDN0001148577)

    Plasmid

    #75638

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EIF2AK2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EIF2AK2 (EIF2AK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. P445-FBP_17

    Plasmid

    #139708

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is flower specific, circadian controlled

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pPhODO1:NnLuz:tAtuOcs
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    various

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX458_Ruby

    Plasmid

    #110164

    Purpose

    Cas9 from S. pyogenes with Ruby2, and cloning backbone for sgRNA

    Depositor

    Philip Hublitz

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and Ruby2

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. EIF2AK2 gRNA (BRDN0001146342)

    Plasmid

    #75637

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EIF2AK2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EIF2AK2 (EIF2AK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMOD_C'0000a

    Plasmid

    #127226

    Purpose

    Empty Backbone for golden gate cloning compatible with Cermak., et al Toolkit.

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMOD_D0000a

    Plasmid

    #127230

    Purpose

    Empty Backbone for golden gate cloning compatible with Cermak., et al Toolkit.

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pegRNA-HEK3_del1-5-NGG

    Plasmid

    #185478

    Purpose

    pegRNA plasmid in order to make a 5 basepair deletion at the HEK3 locus, uses an NGG-PAM site.

    Depositor

    Sabine Fuchs

    Article

    Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

    Insert

    HEK3_del1-5-NGG pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TFORF0701

    Plasmid

    #143065

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PAXBP1 (PAXBP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge