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Showing: 1951 - 2000 of 6924 results

  2. SARS-CoV-2 Spike-d18

    Plasmid
    #164583
    Purpose
    A mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.
    Depositor
    Jason Sheltzer
    Insert
    SARS-CoV-2 Spike (S SARS-CoV-2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deleted amino acids 1256-1273
    Promoter
    EF1a
    Available since
    Jan. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    164583-plasmid-map-sequence-id-322362

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  25. hU6-DR_BsmBI_DR-EFS-RfxCas13d-NLS-2A-Puro-WPRE CasRx pre-gRNA backbone

    Plasmid
    #219823
    Purpose
    EF1α-driven expression of RfxCas13d in mammalian cells. For cloning of pre-guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. (Adapted from plasmid #138147)
    Depositor
    Philipp Maass
    Insert
    RfxCas13d
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core promoter
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219823-plasmid-map-sequence-id-441127

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  34. pSin4 UAP1 F383G

    Plasmid
    #169891
    Purpose
    Used for generation of stable mammalian cell lines expressing UAP1 with with F383G mutation
    Depositor
    Jennifer Kohler
    Article
    Capota et al Curr Protoc. 2021 Jul;1(7):e201. doi: 10
    Insert
    UDP-N-acetylhexosamine pyrophosphorylase (UAP1 Human)
    Use
    Lentiviral
    Tags
    None
    Expression
    Mammalian
    Mutation
    Changed Phenylalanine (F) at position 383 to glyc…
    Promoter
    Available since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169891-plasmid-map-sequence-id-333152

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  41. pGEMS-II-PEST

    Plasmid
    #204766
    Purpose
    GEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)
    Depositor
    Kate Meyer
    Article
    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Insert
    EGFP-PEST-m6Alinker-DHFR
    Use
    Tags
    Expression
    Mutation
    mutated all RACs in EGFP to RGC/RCC
    Promoter
    CMV
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    204766-plasmid-map-sequence-id-408653

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Showing: 1951 - 2000 of 6924 results