We narrowed to 7,122 results for: ef1a

1. pLV Cnep1r1-V5

   Plasmid

   #175133

   Purpose

   Lentiviral expression of mouse Cnep1r1-V5

   Depositor

   Larry Gerace

   Insert

   Cnep1r1 (Cnep1r1 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLV H2afy-V5

   Plasmid

   #175122

   Purpose

   Lentiviral expression of mouse Macroh2a1-V5

   Depositor

   Larry Gerace

   Insert

   Macroh2a1 (Macroh2a1 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSBbi-Puro_LPAR_AKAR-mut

   Plasmid

   #125207

   Purpose

   SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted FRET-based PKA activity reporter

   Depositor

   Eric Davis

   Insert

   LPAR_AKAR-mut
   

   Use

   Transposon

   Tags

   EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…

   Expression

   Mammalian

   Mutation

   FoxO1 Thr-24 changed to Val rendering this inacti…

   Promoter

   EF1a/RPBSA

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEF-GalNAcT2-mGold

   Plasmid

   #218964

   Purpose

   Expresses full length GalNAcT2-mGold in mammalian cells

   Depositor

   Benjamin Glick

   Insert

   GalNAcT2-mGold (GALNT2 Human)
   

   Use

   Tags

   mGold

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEF-GalNAcT2-msYFP2

   Plasmid

   #218963

   Purpose

   Expresses full length GalNAcT2-msYFP2 in mammalian cells

   Depositor

   Benjamin Glick

   Insert

   GalNAcT2-msYFP2 (GALNT2 Human)
   

   Use

   Tags

   msYFP2

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEF-GalNAcT2-msGFP2

   Plasmid

   #218965

   Purpose

   Expresses full length GalNAcT2-msGFP2 mammalian cells

   Depositor

   Benjamin Glick

   Insert

   GalNAcT2-msGFP2 (GALNT2 Human)
   

   Use

   Tags

   msGFP2

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. lenti-Cas9-Exo-Blast-BFP

   Plasmid

   #196721

   Purpose

   Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.

   Depositor

   Bernhard Schmierer

   Insert

   Cas9-Exo1-T2A-BSD-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLV V5-Eva1b

   Plasmid

   #175158

   Purpose

   Lentiviral expression of V5-tagged mouse Eva1b

   Depositor

   Larry Gerace

   Insert

   Eva1b (Eva1b Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLV Cenpv-V5

   Plasmid

   #175125

   Purpose

   Lentiviral expression of mouse Cenpv-V5

   Depositor

   Larry Gerace

   Insert

   Cenpv (Cenpv Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSBbi-Pur-AktAR2-D

    Plasmid

    #125196

    Purpose

    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of AktAR2 variant of FRET-based AKT activity reporter

    Depositor

    Eric Davis

    Insert

    AktAR2-D
    

    Use

    Transposon

    Tags

    6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…

    Expression

    Mammalian

    Mutation

    FoxO1 Thr-24 changed to Val rendering this inacti…

    Promoter

    EF1a/RPBSA

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p11L2w

    Plasmid

    #37331

    Depositor

    John Schiller

    Article

    Kieback et al Virology. 2006 Feb 5;345(1):199-208. Epu

    Insert

    HPV11 L2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. iF53 PB-Zim3-dCas9-mScarlet-Hygro

    Plasmid

    #204726

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Aiolos AA130-189 in Artichoke

    Plasmid

    #74451

    Purpose

    Monitor post-translational degradation of Aiolos AA130-189 (Lentiviral, SFFV.AiolosAA130-189-17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

    Depositor

    Benjamin Ebert

    Insert

    Aiolos (IKZF3) AA130-189 (IKZF3 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    SFFV and EF1a

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. iF58 PB-Zim3-dCas9-mScarlet-Puro

    Plasmid

    #204728

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKAM-E-Flag-GFP

    Plasmid

    #101866

    Purpose

    Lentiviral Evi-1-Flag with GFP marker

    Depositor

    Archibald Perkins

    Insert

    Evi-1 (Mecom Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLV Cbx1-V5

    Plasmid

    #175106

    Purpose

    Lentiviral expression of mouse Cbx1-V5

    Depositor

    Larry Gerace

    Insert

    Cbx1 (Cbx1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. iD20 LoxStopLox-Zim3-dCas9-mApple-hygro

    Plasmid

    #204720

    Purpose

    Cre-inducible CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV Lnpk-V5

    Plasmid

    #175121

    Purpose

    Lentiviral expression of mouse Lnpk-V5

    Depositor

    Larry Gerace

    Insert

    Lnpk (Lnpk Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ELL1 CtoA

    Plasmid

    #127279

    Purpose

    Testing if ELL1 C to A mutation knock out the action on Myc as it does in human

    Depositor

    Christine Milcarek

    Insert

    ELL1 (NM_007924 plus CEYLHNK to AEYLHNK) (ELL Mouse)
    

    Use

    Tags

    Twin strep-tag

    Expression

    Mammalian

    Mutation

    CEYLHNK to AEYLHNK by Q5 mutagenesis

    Promoter

    EF1a

    Available since

    Nov. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV-hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110)-IRES-Blast

    Plasmid

    #193139

    Purpose

    Expresses bicistronic P2A construct of C-CRL4Cdt2 reporter(hCdt1(1-100)ΔCy-mCherry-) and APC/C reporter (hGeminin(1-110)) with blasticidin resistance

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110) (CDT1, GMNN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…

    Promoter

    EF1a

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pKAM-E-Flag-BFP

    Plasmid

    #101867

    Purpose

    Lentiviral Evi-1-Flag with dual BFP and puro markers

    Depositor

    Archibald Perkins

    Insert

    Evi-1 (Mecom Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKAM-ME-Flag-BFP

    Plasmid

    #101868

    Purpose

    Lentiviral Mds1-Evi-1-Flag with dual BFP and puro markers

    Depositor

    Archibald Perkins

    Insert

    Mds1-Evi-1 (Mecom Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. MLCK_pLVX-EF1-Cry2-mCherry

    Plasmid

    #64369

    Purpose

    lentiviral expression of Myosin Light Chain Kinase and Cry2-mCherry

    Depositor

    Matthieu Piel

    Insert

    Myosin Light Chain Kinase (MYLK Human)
    

    Use

    Lentiviral

    Tags

    Cry2-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

24. PB-TO-MYOD1-shOct4

    Plasmid

    #182309

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into skeletal muscle cells via MYOD1 and shOCT4 expression

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Inserts

    MYOD1-shOct4 (MYOD1 Human)
    puro-T2A-mycNLS-mTagBFP2
    

    Use

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    shOct4

    Promoter

    EF1a and TRE3G

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJW1511

    Plasmid

    #62365

    Purpose

    pEF1a-puro-T2A-ATHmmuRPL10A

    Depositor

    Jonathan Weissman

    Article

    Jan et al Science. 2014 Nov 7;346(6210):1257521. d

    Insert

    Puro-T2A-AVITEVHAmmuRPL10A
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. iE61 PB-Zim3-XTEN-dCas9-mScarlet-puro-BFP

    Plasmid

    #204722

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AAV-hACE2-cMYC-Flag

    Plasmid

    #158957

    Purpose

    Used for expression of human ACE2 with AAV packaging

    Depositor

    Akiko Iwasaki

    Insert

    Human ACE2 (ACE2 Human)
    

    Use

    AAV

    Tags

    Flag and MYC

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. lentiGuide-Puro PRMT5-CRISPRi

    Plasmid

    #164637

    Purpose

    lentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2

    Depositor

    Günter Schneider

    Insert

    target guide sequence PRMT5 inhibition (PRMT5 Human)
    

    Use

    CRISPR and Lentiviral; Interference

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pKER-mCerulean

    Plasmid

    #86622

    Purpose

    Expresses mCerulean from the EF1a promoter in mammalian cells

    Depositor

    Michele Calos

    Article

    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

    Insert

    mCerulean
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEF1-puro/hpb1

    Plasmid

    #59976

    Purpose

    integrin b1 short headpiece expression (beta-I, hybrid, PSI and I-EFG1 domains) expression

    Depositor

    Timothy Springer

    Insert

    human integrin b1 headpiece (ITGB1 Human)
    

    Use

    Tags

    Base coiled-coil and His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a promoter

    Available since

    Sept. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

31. pEF1-puro/hpb1(short)

    Plasmid

    #59973

    Purpose

    integrin b1 short headpiece expression (beta-I, hybrid, PSI domains) expression

    Depositor

    Timothy Springer

    Insert

    human integrin b1 (short) (ITGB1 Human)
    

    Use

    Tags

    Base coiled-coil and His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a promoter

    Available since

    Sept. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

32. DOX: FSHB-t2a-GFP

    Plasmid

    #205595

    Purpose

    an overexpression vector of the killifish FSHB under doxycycline control

    Depositor

    Itamar Harel

    Article

    Moses et al eLife. 2023 Oct 24;12:e85960. doi: 10.75

    Insert

    FSHB
    

    Use

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. iF67 PB-Zim3-XTEN-dCas9-mScarlet-Bsd-Neo-mAmetrine

    Plasmid

    #204732

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. iF60 PB-LoxStopLox-Zim3-dCas9-mScarlet-puro-BFP

    Plasmid

    #204729

    Purpose

    Cre-inducible CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. iF57 PB-FrtStopFrt-Zim3-dCas9-mScarlet-puro-BFP

    Plasmid

    #204727

    Purpose

    Frt-inducible CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. iE65 PB-Zim3-XTEN-dCas9-mApple-puro-BFP

    Plasmid

    #204724

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. iF51 PB-Zim3-dCas9-mScarlet-Hygro-Neo-SnapTag

    Plasmid

    #204725

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSBbi-Pur-Lyn-AktAR2-EV-D

    Plasmid

    #125200

    Purpose

    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted Lyn-AktAR2-EV variant of FRET-based AKT activity reporter

    Depositor

    Eric Davis

    Insert

    Lyn-AktAR2-EV-D
    

    Use

    Transposon

    Tags

    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…

    Expression

    Mammalian

    Mutation

    FoxO1 Thr-24 changed to Val rendering this inacti…

    Promoter

    EF1a/RPBSA

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-EF1α1.1-Archon1-KGC-GFP-ER2

    Plasmid

    #115890

    Purpose

    AAV-mediated expression of Archon1-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version). Using SV40 signal.

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon1-KGC-GFP-ER2
    

    Use

    AAV

    Tags

    ER2, GFP, and KGC

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a（1.1kb short version)

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSBbi-Pur

    Plasmid

    #60523

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pSBbi-Bla

    Plasmid

    #60526

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: blasticidin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. PB-TO-NFIA.1-SOX9

    Plasmid

    #182310

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Inserts

    NFIA-T2A-SOX9 (NFIA, SOX9 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Piggybac

    Tags

    None and T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and TRE3G

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. CROPseq-3P5P

    Plasmid

    #219680

    Purpose

    CROPseq vector enabling gRNA detection via mRNA-based capture (CROPseq), 3'-based direct capture using 10x capture sequence 1 or 5'-based enrichment using a custom reverse transcription primer

    Depositor

    Christoph Bock

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    EF1a-Puro-P2A-mCherry2-WPRE-hU6-gRNA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. CROPseq-puro-v2

    Plasmid

    #127458

    Purpose

    Entry plasmid for guide cloning

    Depositor

    Paul Blainey

    Article

    Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pSBbi-GP

    Plasmid

    #60511

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pSBbi-RP

    Plasmid

    #60513

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. LentiCRISPRv2R

    Plasmid

    #167932

    Purpose

    lentiCRISPRv2 variant with mScarlet-I fluorescence marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. pSBbi-RH

    Plasmid

    #60516

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and hygromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pSBbi-BP

    Plasmid

    #60512

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pSBbi-Neo

    Plasmid

    #60525

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: neomycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge