We narrowed to 13,644 results for: sequence
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Plasmid#243798PurposeEncodes for mammalian expression of TVMV, TEV and HRV-3C protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.DepositorInsertTVMV, TEV and HRV-3C proteases
ExpressionMammalianAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
betaTrCP mVenus mutant reporter
Plasmid#192459PurposeFluorescent reporter for beta-TrCP with a non-degradable mutation. Used as a control.DepositorInsertCdC25B
UseLentiviralTagsSV40 NLS and mVenusExpressionMammalianMutationaa198-338 of CDC25B. betaTrCP degron sequence (aa…PromoterEF1alphaAvailable SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
RAMA-bio-His
Plasmid#50737PurposeExpresses enzymatically monobiotinylated full length RAMA ectodomainDepositorInsertRAMA
TagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable SinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Cys-ELP(40nm)-LPETGG
Plasmid#90475PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGGDepositorInsertCys-ELP(40nm)-LPETGG
TagsCysteine and Sortase-TagExpressionBacterialPromoterT7Available SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDS275
Plasmid#34972PurposeExpresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(T406A, T407A, I532A mutations).DepositorInsertMid2(SS/TM)-GFP-LOVpep
ExpressionYeastMutationT406A, T407A, I532AAvailable SinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pY2ShHELIX_sgRNA_entry (CJT30)
Plasmid#181781PurposeExpresses Y2-ShHELIX containing a nicking Y2 I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertY2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
ExpressionBacterialMutationY2-nAniI = F13Y, S111Y, K227M, F80K, L232KPromoterLac and J23119Available SinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRvTUB-mEGFP-NLS
Plasmid#205011PurposeThe vector contains 1kbp of the upstream and downstream regions of the tubulin gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the tubulin gene from R. varieornatus
UseTardigrade expressionAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-FLAG-hEcto/Tif1g
Plasmid#20902DepositorInsertEctodermin (TRIM33 Human)
TagsFLAGExpressionMammalianMutationN-terminal deletion of 37 aminoacids; Ectodermin …Available SinceApril 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pENTR-p38KTRmCerulean3
Plasmid#59149PurposeORF encoding p38 KTR mCerulean3 flanked by Gateway sequencesDepositorInsertp38 Kinase Translocation Reporter (MAPK14 Mouse, Human)
Available SinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pB2-neo-TET
Plasmid#51287Purposeexpresses the rodent SINE, B2, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassetteDepositorInsertsine B2
TagsneoTET cassette with a tetrahymena self-splicing …ExpressionMammalianPromoterenhancer from 7SL geneAvailable SinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYL322d1/N-eGFP
Plasmid#183151PurposePlant transient expression vector containing eGFP tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of eGFP.DepositorTypeEmpty backboneExpressionPlantAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
tet_pLKO.1_puro_shLuc
Plasmid#136587PurposeExpresses an inducible short hairpin targeting firefly luciferase sequenceDepositorInsertshLuc
UseLentiviralExpressionMammalianAvailable SinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-LifeAct-FRB-Venus-173-N
Plasmid#87616PurposeFRB-tagged N-terminal Venus (YFP) fragment for BiFC; localizes to actin cytoskeleton.DepositorInsertLifeAct-FRB-Venus173-N
Tags17-aa sequence from Abp140 (aka "Lifeact&quo…ExpressionMammalianPromoterCMVAvailable SinceFeb. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28b-His-hCE
Plasmid#112710PurposeExpresses N-terminally His-tagged human CE/RNGTT in bacterial cellsDepositorInsertRNGTT (RNGTT Human)
Tags6xHis and Stop codons placed after RNGTT sequence…ExpressionBacterialMutationR594HPromoterT7Available SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFH2.30_Gag-MCP-dpol-T2A-eGFP
Plasmid#205538PurposeExpress HIV Gag-MCP-dpol-T2A-eGFPDepositorInsertHIV Gag-MCP-dpol
ExpressionMammalianMutationpol frameshift sequence recodedPromoterCMVAvailable SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCD/NL-BH∆1
Plasmid#41791PurposePackaging construct contains Gag and Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding RevDepositorInsertHIV-1 Gag/Pol and RRE sequences
ExpressionMammalianAvailable SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-GGG-ELP(40nm)-Cys
Plasmid#91571PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal CysteineDepositorInsertGGG-ELP(40nm)-Cys
TagsCysteine and Sortase-TagExpressionBacterialPromoterT7Available SinceFeb. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRelA'
Plasmid#175595PurposeTet inducible, KAN Resistant, non labelled synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.DepositorInsertCatalytic domain of E.coli relA gene.
ExpressionBacterialMutationOnly the catalytic domain of the enzyme is used (…Available SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH_SBP-EGFP-Ecadherin
Plasmid#65290PurposeEcadherin reporter only (no hook) (RUSH system)DepositorInsertEcadherin
UseLentiviralTagsEGFP and IL-2 Signal Sequence and Streptavidin Bi…PromoterCMVAvailable SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sapphireCKAR(T/A)
Plasmid#118469PurposeNegative-control mutant for sapphireCKAR biosensor.DepositorInsertsapphireCKAR(T/A)
Tags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable SinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only